Which fluorometric quantification method to use for quantifying input DNA for the Infinium Assay?

The input DNA for the Infinium workflow must be double-stranded. A fluorometric quantification method, such as Qubit or PicoGreen, is recommended to accurately measure the amount of double-stranded DNA (dsDNA) extracted from fresh/frozen/FFPE samples. If using Qubit, use the quantification assay kits specific for dsDNA.

DNA quantification results from UV spectrophotometric methods, such as Nanodrop, do not accurately reflect the concentration of double-stranded DNA and are not recommended. Using the quantification values from the Bioanalyzer or Tapestation is not advised.

Additional guidelines are provided in the following resources:

• Guidelines for Sample Preparation for Infinium Genotyping Assay DNA input

• DNA Input Guidelines for Infinium MethylationEPIC Arrays

• Infinium Assay Modifications for FFPE samples for Genotyping Arrays

For any feedback or questions regarding this article (Illumina Knowledge Article #8332), contact Illumina Technical Support [e-mail].