Running DRAGEN analyses with ORA files as input
DRAGEN ORA files can be used directly as input for the map/align step or they can be decompressed to FastQ files first. Here are a few scenarios which use ORA files as input and the flags that need to be included in said analyses.
Input | Map/Align | FastQ Output | Flags to be used | ||
ORA | Yes | No |
| ORA | No | Yes |
| ||
--ora-reference [path to the ORA reference] --enable-map-align true | |||||
--ora-reference [path to the ORA reference] --enable-map-align true | |||||
ORA | No | Yes |
| | ORA | Yes | Yes |
| ||
--ora-reference [path to the ORA reference] --enable-ora true --ora-decompress true --enable-map-align false | |||||
--ora-reference [path to the ORA reference] --enable-ora true --ora-decompress true --enable-map-align false | |||||
ORA | Yes | Yes |
| More information about ORA compression/decompression can be found in the user guide.
| ||
--ora-reference [path to the ORA reference] --enable-ora true --ora-decompress true --enable-map-align true | |||||
For any feedback or questions regarding this article (Illumina Knowledge Article #7955), contact Illumina Technical Support techsupport@illumina.com. |
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