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  • Instrumentation
    • General
      • FAQ
        • Are the RFID tags for the flow cell and reagent cartridge linked?
        • Differences in the percent clusters passing filter on patterned and non patterned flow cells
        • End of Life Schedule for HiSeq Platforms
        • How is PhiX alignment calculated by RTA (Real Time Analysis)?
        • How to determine the appropriate kit configuration to use based on the data needed?
        • Illumina reagent shipment packaging FAQs
        • Is it possible to remove a library pool from a sequencing reagent cartridge?
        • Procedures for cleaning flow cells for Illumina sequencers
        • Reagent Expiration Date Definition
        • Secure communication protocols on Illumina systems
        • Steps if insufficient wash solution was used in a manual wash on the NextSeq 500/550 or MiniSeq
        • What are Illumina's Qualification Service Offerings
        • Which sequencers use patterned vs. nonpatterned flow cells?
      • Reference Material
        • Adding Illumina to TeamViewer allowlist
        • Antivirus recommendations for Illumina Sequencing Instruments
        • Approximate sizes of sequencing run output folders
        • Best practices for getting the lab back up and running after an extended shutdown
        • Best practices for low diversity sequencing on the NextSeq 500/550 and MiniSeq systems
        • Best practices for maintaining and cleaning Illumina sequencing system wash cartridges
        • Best practices for maintaining the computer on Illumina sequencing systems
        • Best practices to improve data yield when using patterned flow cells on the HiSeq 3000/4000/X
        • Bluekeep and DejaBlue two vulnerabilities of the Remote Desktop Protocol within Microsoft Windows
        • Calculating Percent Passing Filter for Patterned and Nonpatterned Flow Cells
        • Chemistry and Imaging on NovaSeq 6000
        • Cluster density considerations when migrating Illumina libraries between sequencing platforms
        • Cluster density guidelines for Illumina sequencing platforms using non patterned flow cells
        • Decontaminating Illumina instruments for novel coronavirus (2019 nCoV)
        • Diagnosing Suboptimal Clustering in Nonpatterned Flow Cells Video
        • Diagnosing Suboptimal Clustering in Patterned Flow Cells Video
        • Does my sequencing run look good?
        • End to end sequencing and microarray training at Illumina Solutions Centers in the Americas
        • Features of Illumina Advantage (IA former TG) product
        • Focus images on the MiniSeq and NextSeq 500/550
        • Group Policy Object (GPO) reports
        • Heat output specifications for Illumina sequencers
        • HiSeq 3000/4000 Introduction Support Webinar Video
        • How Do I Prepare for My Illumina Instrument Delivery? Video
        • How many cycles of SBS chemistry are in my kit?
        • How much PhiX spike in is recommended when sequencing low diversity libraries on Illumina platforms?
        • How to Clean Illumina Sequencer Wash Cartridges Video
        • How to Disable Cloud Login Feature on the MiSeq i100 and NovaSeq X Series
        • How to Requeue an Analysis on BaseSpace Sequence Hub When No Changes to the Sample Sheet are Needed
        • How to achieve more consistent cluster density on Illumina sequencing platforms
        • How to check .NET version on Windows instruments
        • How to connect benchtop instruments and Local Run Manager to a network location
        • How to disable and add exceptions to the Software Restriction Policies for TeamViewer in Windows 10
        • How to identify the IP and MAC address of a sequencing instrument
        • How to identify the UNC path of a network server or location.
        • How to import a pre existing index kits into Local Run Manager v3 and v4
        • How to interpret clusters passing filter in run metrics
        • How to make sure Universal Copy Service is configured to Log On as a standard (non admin) account
        • How to perform System Check on the NovaSeq X Series
        • How to requeue and import run data for reanalysis with Local Run Manager
        • How to restart RTA on HiSeq 2500 and MiSeq systems
        • How to restart a sequencer after a power outage?
        • How to set up a PhiX validation run with Local Run Manager
        • How to thaw and store sequencing reagents for optimal performance
        • How to update Windows 10 account credentials on Benchtop Instruments
        • IT Requirements for Implementing Illumina Proactive Video
        • Illumina 测序平台的最大读长
        • Illumina测序平台上每个测序步骤的运行时间预测
        • Illumina测序平台测试Run中PhiX的上机浓度
        • Illumina测序系统废液中甲酰胺的终浓度是多少?
        • Illumina非Pattern Flow Cell测序平台簇密度推荐指南
        • Impact of ammonium based cleaning products on sequencing run performance
        • Index color balancing for the NextSeq 500/550 and MiniSeq systems
        • Instrument Service Contract Plans
        • Instrument maintenance and shutdown procedures for extended site closures
        • Introducing TeamViewer for Remote Desktop Share with Illumina Support Teams
        • Introduction to Illumina Sequencing By Synthesis Support Webinar Video
        • Investigation of Log4j Vulnerability with Illumina ProActive Portal
        • Investigation of Log4j Vulnerability with Illumina instruments
        • Maximum read length for Illumina sequencing platforms
        • Network Output Path Requirements in Local Run Manager and Universal Copy Service
        • NextSeq500/550和Miniseq平台进行低多样性文库测序的最佳操作
        • NextSeq和MiniSeq测序系统上index混合的指导方针
        • PhiX loading concentrations for validation runs on Illumina sequencing platforms
        • Plotting %Occupied by %Pass Filter to optimize loading for the NovaSeq 6000 and iSeq 100 Video
        • Quality Scores for Next Generation Sequencing
        • Run time estimates for each sequencing step on Illumina sequencing platforms
        • Security updates to address Windows Print Spooler Remote Code Execution Vulnerability
        • Sequencing Analysis Viewer (SAV) A Beginner's Guide Support Webinar Video
        • Sequencing Considerations for Low Diversity Samples Support Webinar Video
        • Serial Number locations on Illumina sequencers
        • Sharing instrument performance data with Illumina Support
        • Shelf life for Illumina reagent products
        • System checks for Illumina sequencing platforms
        • Technical resources for the HiSeq 3000 and 4000 systems
        • Troubleshooting "Cannot initiate communication with Universal Copy Service" error
        • Troubleshooting "Could not find a part of the path" errors on the NextSeq500/550 and MiniSeq
        • Troubleshooting Bit Error on the NextSeq 500/550 and MiniSeq
        • Troubleshooting a blank BaseSpace screen or The request timestamp is in the future during run setup
        • Uninterruptible Power Supply (UPS) Models by Instrument
        • Using the MyIllumina dashboard to view instrument yield, Reads PF, and Q30 data as trends over time
        • What is nucleotide diversity and why is it important?
        • What is the final formamide concentration in the waste solution of Illumina sequencing systems?
        • Why sequencing 26 or more cycles in Read 1 is recommended
        • 为什么Read1测序读长推荐至少需要26个循环?
        • 什么是核苷酸多样性,它为什么十分重要?
        • 在Illumina平台进行低多样性文库测序时,应该掺入多少PhiX?
        • 场地长期关闭或仪器长期不用时illumina仪器的维护与关机程序
        • 如何在HiSeq 2500和MiSeq系统上重启RTA
        • 如何在Illumina测序平台上获得更稳定的簇密度
        • 如何在搭载Windows 7 Professional系统的Illumina 仪器上安装相应的扩展安全更新(ESU)程序?
        • 如何更新UCS和LRM在Windows10系统中的凭据 (针对装有NCS v4.0.1+, MNCS v2+, MCS v4+的平台)
        • 我所购买的SBS试剂盒最多能够满足多少个循环的测序?
        • 测序运行输出文件夹的近似大小
        • 通过% Occupied和% Pass Filter散点图优化NovaSeq 6000和iSeq 100上样浓度
      • Troubleshooting
        • AWS deprecation of TLS below v1.2 & impact to instruments connecting to Illumina Connected Services
        • Failed to load BaseSpace Cloud API URL from configuration error on the NextSeq 500/550 and MiniSeq
        • How to Troubleshoot a "no base calls are available for its lane(s)" error in Local Run Manager
        • How to change sbsuser credential from sbsadmin account on Windows instruments
        • How to requeue analysis using only an index 2 (i5) read
        • How to safely remove or eject external hard drives on Illumina platforms with Windows 10
        • How to troubleshoot a Database Backup Secret has not been set warning in Local Run Manager
        • How to troubleshoot unable to find patterned fiducials errors on the HiSeq 3000/4000 platforms
        • Investigating Network Data Copy Performance from Illumina Products on Linux OS
        • Mitigation of the 'Follina' vulnerability in Microsoft Support Diagnostic Tool (MSDT)
        • Optimizing Cluster Density Support Webinar Video
        • Plotting %Occupied by %PF to optimize loading for the NovaSeq X Series, MiSeq i100 Series, NovaSeq 6000, and iSeq 100
        • Supported versions of Server Message Block (SMB) on Illumina Platforms
        • Troubleshooting "Failed to communicate with RTA after 4 attempts" on the MiniSeq or NextSeq 500/550
        • Troubleshooting 'Recipe file does not exist at' errors when setting up 1.5B runs on the NovaSeq X Plus
        • Troubleshooting BaseSpace connectivity issues on sequencing instruments
        • Troubleshooting Blue Screen errors related to the CrowdStrike update
        • Troubleshooting Control Software stuck "Waiting for Safe State" on MiniSeq and NextSeq 500/550
        • Troubleshooting HTTP 503 Error when trying to access LRM after a Windows Credential Update
        • Troubleshooting Issues during Instrument Shutdown on the NovaSeq X Series
        • Troubleshooting Runs not Uploading to Cloud on the NovaSeq X and MiSeq i100 Series
        • Troubleshooting Windows OS Crashes or Blue Screen Errors on Illumina Instruments
        • Troubleshooting low disk space issues in the /var/log/ volume on NextSeq 1000/2000 and NovaSeq X/X Plus
        • Troubleshooting unable to login to Windows based sequencing system
    • iSeq 100
      • FAQ
        • Are custom primers loaded as a pool or individually on the iSeq 100?
        • Are custom primers recoverable after loading into the iSeq 100 reagent cartridge?
        • Are washes required on the iSeq 100?
        • Can Local Run Manager be used to plan iSeq 100 runs with custom sequencing primers?
        • Can PhiX be sequenced on the iSeq 100 when using custom primers?
        • Can custom primers be spiked into the Illumina primers within the iSeq 100 reagent cartridge?
        • Can custom sequencing primers be used on the iSeq 100?
        • Can iSeq 100 runs with custom primers be analyzed in Local Run Manager or BaseSpace Sequence Hub?
        • Does Illumina offer Preventative Maintenance (PM) for iSeq 100 system?
        • Does the iSeq 100 have any special moving considerations?
        • How many runs can be stored on the iSeq 100?
        • How much liquid waste is inside an iSeq 100 cartridge?
        • How to determine whether custom primers were selected on the iSeq 100 once the run is completed?
        • How to dilute custom primers for use on the iSeq 100?
        • How to dispose of iSeq 100 reagents?
        • How to minimize the iSeq 100 Control Software?
        • How to turn audio on or off on the iSeq 100?
        • Lifespan and maximum usage of the iSeq 100 System test kit
        • What are the available run setup modes for iSeq 100?
        • What does the iSeq 100 status bar color mean?
        • When do %>Q30 and %Pass Filter metrics become available for iSeq 100 runs?
      • Reference Material
        • Air Filter replacement on the iSeq 100
        • Chemistry and Imaging on iSeq 100
        • Consumable Catalog Numbers for the iSeq 100
        • Custom primer use workflow for iSeq 100 control software version 3.0
        • File Paths on the iSeq 100
        • How To Enable/Disable Illumina Proactive on iSeq 100
        • How to clear hard drive space on the iSeq 100
        • How to enable Wi Fi on the iSeq 100
        • Installing and using Local Run Manager Modules on the iSeq 100
        • Instructions for installing the iSeq Control Software
        • Library denaturation and compatibility for the iSeq 100 system
        • Library loading concentration considerations for the iSeq 100
        • Power cycling the iSeq 100
        • Reagent stability and thawing for the iSeq 100
        • Resources for iSeq 100 Control Software v3.0
        • Running a System Check on an iSeq 100
        • Software Restriction Policy (SRP) on the iSeq 100
        • Software update best practices on the iSeq 100
        • Specifications for the iSeq 100
        • Step by step instructions for sequencing library QC with the iSeq 100 System
        • Technical resources for the iSeq 100
        • Troubleshooting initialization failed because the API key is blank error the iSeq 100
        • Troubleshooting running low diversity samples with low PhiX spike in on the iSeq 100
        • User accounts for the iSeq 100
        • iSeq 100 Introduction Support Webinar Video
        • iSeq 100 Run Quality and Best Practices Support Webinar Video
        • iSeq 100 Sequencing System: Support Resources Video
        • iSeq 100 Sequencing System: Troubleshooting and Support Video
      • Troubleshooting
        • Blank BaseSpace login screen during run setup in control software on the iSeq 100
        • Clustering failures on the iSeq 100
        • Flow cell heater is out of specification error on an iSeq 100
        • How to identify when an air filter was changed on the iSeq 100.
        • Restore to Factory Settings Option on iSeq 100
        • System error detected please power cycle and perform system checks error on the iSeq 100
        • Troubleshooting "GenerateFASTQ 500 Internal Server Error" in Local Run Manager hosted on the iSeq 100
        • Troubleshooting "Initialization failed due to a software connectivity problem." on the iSeq 100
        • Troubleshooting Black Chromium Screen on the iSeq 100
        • Troubleshooting iSeq 100 pre run check “Insufficient disk space/Not enough space on D:\” error
        • Troubleshooting unable to locate D:\ drive or cartridge failed to unload error on iSeq 100
    • MiniSeq
      • FAQ
        • Can Flow Cell Registration be Disabled for Pre Run Checks on the MiniSeq?
        • Can Sequencing Analysis Viewer be used with the MiniSeq System to view sequencing metrics?
        • Can a previous MiniSeq run in Local Run Manager v1 be used to create a new run?
        • How long does a MiniSeq system check take to complete?
        • How many extra cycles are included in MiniSeq reagent cartridges?
        • Run setup modes for the MiniSeq
        • What does MiniSeq status bar color mean?
        • What is the maximum read length supported in one direction on a MiniSeq run?
        • Which controls should be used when sequencing low diversity libraries on a MiniSeq?
      • Reference Material
        • Consumable catalog numbers for the MiniSeq and total SBS cycles available
        • Custom primers on the MiniSeq
        • Enabling automatic reagent purge on the MiniSeq
        • Enabling saving thumbnail images on the MiniSeq
        • Estimated time for sequencing steps on the MiniSeq
        • File Paths for the MiniSeq Control Software v1.2
        • File Paths for the MiniSeq Control Software v2.0
        • Hard drives and computer of the MiniSeq
        • How to Perform a System Check on the MiniSeq
        • How to Power Cycle the MiniSeq
        • How to enable/disable Illumina Proactive on the MiniSeq
        • How to install custom recipes on MiniSeq Control Software v2.x
        • Information on Run Copy Service (RCS) on the MiniSeq
        • Introducing the MiniSeq Rapid Reagent Kit
        • Local Run Manager v1.3 for MiniSeq How to use a custom library prep kit
        • Low diversity sequencing on the MiniSeq
        • MiniSeq Control Software v2.0 “Invalid Role” error with Local Run Manager v2.4.1
        • MiniSeq Introduction Support Webinar Video
        • MiniSeq Reagent Kit Component Dimensions
        • MiniSeq system components overview
        • Pre run checks on the MiniSeq
        • Saved thumbnail options and how thumbnail images are named on MiniSeq
        • Specifications for the MiniSeq
        • Thawing and Stability Guidelines for MiniSeq Reagent Kits
        • Upgrading to MiniSeq Control Software v2
        • User supplied consumables for the MiniSeq
        • Washes on the MiniSeq
      • Troubleshooting
        • Blank BaseSpace login screen during run setup on MiniSeq Control Software v2
        • Chemistry and Imaging on MiniSeq
        • Import run option is not available following Local Run Manager Upgrade to 1.3.1 on MiniSeq
        • MiniSeq Control Software v2.0 not able to display BaseSpace page on the MiniSeq
        • MiniSeq run display anomaly in Sequencing Analysis Viewer with Mid Output Flow Cells
        • Operational Qualifications Results upload prompt at end of run on the MiniSeq
        • Pre Run Check Troubleshooting on the MiniSeq
        • Pre run check stalls on flow cell registration after installation of MiniSeq Control Software v2.0
        • Single Read option not available with Resequencing Analysis for the TruSeq HT kit on the MiniSeq
        • The optimal cluster density and PhiX loading concentration for MiniSeq runs
        • Troubleshooting "Ebox0 Fan is not operating as expected" warnings on the MiniSeq
        • Troubleshooting "Error in Fluidics, LanePump" error on the MiniSeq
        • Troubleshooting "RunCopyService executable does not exist" error in MiniSeq Control Software 1.3.1
        • Troubleshooting "Unable to create Output folder. MiniSeq does not have access to…" on MiniSeq
        • Troubleshooting "Y Stage Initialization Error Operation home failed..." errors on the MiniSeq
        • Troubleshooting Computer reboot hangs with Illumina logo splash screen on MiniSeq
        • Troubleshooting Duplicate analysis folders created for runs with successful LRM analyses on MiniSeq
        • Troubleshooting MiniSeq Z stages are not ready initialization error
        • Troubleshooting MiniSeq fails optical and stage tests "While measuring LED power"/"Failed XY Stage"
        • Troubleshooting RFID block can not be written (due to lock status) Error on the MiniSeq
        • Troubleshooting Timeout waiting 60000 ms within event EventToBlockingConverter error on the MiniSeq
        • Troubleshooting Windows 10 needs to be activated error on the MiniSeq.
        • Troubleshooting a "Syringe Overload" error on the MiniSeq
        • Troubleshooting camera, cluster, and focus errors at cycle 1 on the MiniSeq
        • Troubleshooting socket errors on the MiniSeq
        • Troubleshooting validation errors displaying when opening "module create run" page on the MiniSeq
        • Unable to pass pre run checks with Imaging Limits test on the MiniSeq
        • Wash Error "Binary Part number revision .. byte1 is invalid" on the MiniSeq
    • MiSeq
      • FAQ
        • Can MiSeq Reagents be stored at 80°C rather than 20°C?
        • Can Sequencing Analysis Viewer (SAV) be used with the MiSeq System to view primary analysis results?
        • Can a MiSeq post run wash be performed every week instead of entering standby mode?
        • Can a MiSeq v2 flow cell be used for sequencing with a MiSeq v3 reagent cartridge?
        • Can an entire index be N padded in Miseq Reporter?
        • Can an expired reagent cartridge be used for a MiSeq run?
        • Can secondary analysis run on MiSeq while a run is in progress?
        • Does the MiSeq verify the number of cycles in a planned run with the cycles in the reagent kit?
        • How Long Does the Flow Rate Check Take During the MiSeq Pre Run Checks?
        • How many cycles of sequencing can be performed with the MiSeq reagent kits?
        • How many index cycles can be sequenced on MiSeq?
        • How much template does the MiSeq System use?
        • How to access MiSeq Reporter
        • How to enable email notifications on the MiSeq
        • How to stop a MiSeq run
        • Is it possible to perform a primer rehybridization on a MiSeq?
        • MiSeq Flow cell compatibility for v2 and v3 reagent kits
        • Run setup modes for the MiSeq
        • What are the MiSeq dimensions and placement requirements?
        • What are the lab temperature requirements for the MiSeq?
        • What are the power specifications for the MiSeq?
        • What color of cap on the MiSeq flow cell container corresponds to which type of flow cell?
        • What does the MiSeq status bar color mean?
        • What if I did not name the sample sheet with the MiSeq reagent cartridge barcode number?
        • What is the maximum read lengths and total number of cycles for MiSeq kits?
        • What is the minimum PhiX amount that can be spiked into a run?
        • When do quality scores appear during a sequencing run on the MiSeq?
        • When is the demultiplexing summary generated for analyzed MiSeq runs?
        • Which Illumina library preparation kits are compatible with the MiSeq?
        • Which controls should be used when sequencing low diversity libraries on a MiSeq?
      • Reference Material
        • Best practices for maintaining the computer on a MiSeq
        • Chemistry and imaging on MiSeq
        • Configuring MiSeq Control Software v4 and MiSeq Reporter to write to Samba Shares on a Linux server
        • Consumable Part Numbers for the MiSeq and Total SBS cycles available
        • Custom sequencing primers on the MiSeq
        • Enabling run storage and analysis in BaseSpace for MiSeq runs
        • File paths for the MiSeq
        • Frequently asked questions about upgrading to MiSeq Control Software v4.0
        • How To Power Cycle the MiSeq Video
        • How to Create a Sample Sheet in Local Run Manager v2 and v3
        • How to Denature and Dilute Libraries and Spike in PhiX on the MiSeq Video
        • How to Enable/Disable Illumina Proactive on the MiSeq
        • How to Perform Washes on the MiSeq Video
        • How to Power Cycle the MiSeq
        • How to Prepare a MiSeq Sequencing Reagent Cartridge Video
        • How to Start a Run on the MiSeq Video
        • How to check network settings on the MiSeq
        • How to clean the MiSeq flow cell holder
        • How to configure MiSeq Reporter to use a network location as the default output folder
        • How to find sample sheet templates for MiSeq Control Software (MCS) v4.0 Local Run Manager
        • How to generate RFID bypass codes for the MiSeq
        • How to generate an RFID bypass code in MyIllumina Video
        • How to make sure FASTQ generation occurs in MiSeq Reporter when using BaseSpace for analysis
        • How to navigate the MiSeq Self Service Resources tool
        • How to perform a System Check on the MiSeq
        • How to prepare low yield (< 2 nM) libraries for sequencing on a MiSeq
        • How to properly format and use a custom reference genome in MiSeq Reporter
        • How to set up a PhiX validation run on MiSeq (using software v2.6 and earlier)
        • How to set up the sample sheet for a PhiX validation run on the MiSeq using IEM
        • How to stop and restart RTA on the MiSeq
        • MiSeq RFID Bypass Code生成方法
        • MiSeq Run Quality and Best Practices Support Webinar Video
        • MiSeq Sequencing Reagents Preparation: Flow cell Video
        • MiSeq flow cell tiles by flow cell type
        • MiSeq output and analysis folder quick reference guide
        • MiSeq上开始一个测序反应的时候,遇到Flow Rate检测失败,该如何处理?
        • MiSeq上机测序时如何准备<2nM 的文库
        • Reagent Stability and Thawing Guidelines for MiSeq Sequencing Kits
        • Recommendations for optimal cluster density and PhiX loading concentrations on the MiSeq System
        • Reference images for the MiSeq
        • Run folder sizes and Disk Space on the MiSeq
        • Sample sheet creation for MiSeq Control Software v2.6 and Earlier
        • Specifications for the MiSeq
        • Technical resources for the MiSeq
        • Thumbnail Image naming conventions for the MiSeq
        • Upgrading to MiSeq Control Software v4.0
        • User supplied consumables for sequencing on the MiSeq
        • Wash Notifications on the MiSeq
        • Wash best practices for the MiSeq
        • 如何排查Miseq平台上的第一个循环报错的原因?
      • Troubleshooting
        • Best practices to avoid low cluster density on the MiSeq
        • How to Check if a MiSeq Cartridge was pierced during a run.
        • How to access Windows on the MiSeq if there is black screen at boot up
        • How to recover Local Run Manager v3 Account when locked out
        • How to troubleshoot MiSeq runs taking longer than usual or expected
        • How to troubleshoot The process cannot access the file error on MiSeq
        • Troubleshooting "@@@ Failed to get temperature" error on the MiSeq
        • Troubleshooting "Compensator Move and/or Sensor Failed" errors on the MiSeq
        • Troubleshooting "Conflict" errors at end of MiSeq runs
        • Troubleshooting "Initialize Cameras before capturing images" error on the MiSeq
        • Troubleshooting "Primary Analysis Not Ready" errors during Pre run Checks on MiSeq
        • Troubleshooting "TEC2SERVO unrecognize char" error during MiSeq Control Software initialization
        • Troubleshooting "Unable to communicate with BaseSpace" error when setting up MiSeq run on Windows 10
        • Troubleshooting "Unsuccessfully attempted to launch module type" error with LRMv3 on MiSeq
        • Troubleshooting "Y home sensor never tripped" error on MiSeq
        • Troubleshooting 'Index outside the bounds of the array' error on the MiSeq
        • Troubleshooting Camera exception, error= 360: Failed to perform sBCa on the MiSeq
        • Troubleshooting Cannot get Value value (type Boolean) errors on the MiSeq
        • Troubleshooting Cavro Pump Error on MiSeq
        • Troubleshooting FASTQ Generation failing to start automatically in MiSeq using MiSeq Reporter
        • Troubleshooting FASTQ generation not occurring automatically after a MiSeq run has completed successfully and successfully uploaded to BaseSpace
        • Troubleshooting FPGA RFID ERROR STATUS 2 errors on the MiSeq
        • Troubleshooting FPGA Timeout Errors on the MiSeq
        • Troubleshooting Focus Mirror Error on the MiSeq
        • Troubleshooting Free Disk Space errors during Pre run Checks on MiSeq
        • Troubleshooting Green LED Failed polling LED status error on MiSeq
        • Troubleshooting Invalid Role Errors after Post Run Washes on the MiSeq
        • Troubleshooting LRM error "Valid index kit must be provided for the selected library prep kit"
        • Troubleshooting Lane Pump Errors on the MiSeq
        • Troubleshooting MiSeq "Z Motor The Z stage amp Op amp might not be wired correctly" Error
        • Troubleshooting MiSeq "a valid index kit must be provided for the selected library prep kit" error
        • Troubleshooting MiSeq Control Software (MCS) v4 stalling during initialization
        • Troubleshooting MiSeq Read 2 low intensity and quality scores
        • Troubleshooting MiSeq washes taking extended time to complete
        • Troubleshooting MiSeqOverride.cfg Errors on the MiSeq
        • Troubleshooting Network Connection Active errors during Pre run Checks on MiSeq
        • Troubleshooting PR2 Bottle Not Detected Error on the MiSeq
        • Troubleshooting RFID Not Recognized Error on the MiSeq
        • Troubleshooting RFID cannot be written error on the MiSeq
        • Troubleshooting Reagent Valve errors on the MiSeq
        • Troubleshooting SIPDOWN Errors on the MiSeq
        • Troubleshooting Sample Sheet will not Load into MiSeq Control Software
        • Troubleshooting Sipdown Errors on the MiSeq video
        • Troubleshooting Stalled Flow Rate Check on the MiSeq
        • Troubleshooting Waste Bottle is Full notification on the MiSeq
        • Troubleshooting Y Motor Errors on the MiSeq
        • Troubleshooting Z Motor Positive soft limit too nonlinear at innermost test point on the MiSeq
        • Troubleshooting Z motor errors occurring mid run on the MiSeq
        • Troubleshooting a MiSeq "Consecutive Camera Failure Limit has been Exceeded for this Run" error
        • Troubleshooting a bubble in MiSeq flow cell
        • Troubleshooting access to the path Global\Local Run Manager is denied on MiSeq
        • Troubleshooting an Object Reference Error on MiSeq
        • Troubleshooting best focus errors occurring mid run on the MiSeq
        • Troubleshooting cycle 1 errors on the MiSeq
        • Troubleshooting failed MiSeq system checks
        • Troubleshooting file is not properly formatted warning on the MiSeq
        • Troubleshooting frozen MiSeq Control Software
        • Troubleshooting high cluster density on MiSeq
        • Troubleshooting low cluster density on the MiSeq
        • Troubleshooting mid run shutdowns or reboots on the MiSeq
        • Troubleshooting no intensity for index read on MiSeq
        • Troubleshooting scratched or damaged flow cells on MiSeq
        • Troubleshooting unable to measure flow rate errors on the MiSeq
    • MiSeq i100 Series
      • FAQ
        • When will Live %Demux information be available for a MiSeq i100 Series run?
        • Where to find the MAC Address for the MiSeq i100 Series
      • Reference Material
        • Activating TeamViewer on the MiSeq i100 Series Instruments
        • Considerations for Index color balancing on the MiSeq i100 Series
        • Consumable catalog numbers for the MiSeq i100 Series
        • Estimating Disk Space Utilization on the MiSeq i100 Series
        • File paths on the MiSeq i100 Series
        • Handling and disposal information for used reagents on the MiSeq i100 Series
        • How to Access the Linux Terminal on the MiSeq i100 Series Instruments
        • How to Configure Network Settings for the MiSeq i100 after First Time Setup
        • How to Export Logs on the MiSeq i100 Series
        • How to Power Cycle the MiSeq i100 Series
        • How to Restart File Transfer on the MiSeq i100 Series
        • How to Setup a Remote Connection to Illumina Run Manager on the MiSeq i100 Series Instruments
        • How to Setup the System Health Checks on the MiSeq i100 Series Instruments
        • How to Spike in PhiX for the MiSeq i100 Series
        • How to change passwords for the Control Software accounts on the MiSeq i100 Series Instrument
        • How to retrieve the DRAGEN (FPGA) Serial Number for MiSeq i100 Series Systems
        • How to set up a PhiX validation run on MiSeq i100 Series
        • Instrument reference images for the MiSeq i100 Series
        • Loading Concentration Optimization Recommendations for the MiSeq i100 Series
        • Offline DRAGEN License Installation on the MiSeq i100 Series
        • Planning a Manual Mode Run on MiSeq i100 Series
        • RTA4 Q Score Bin Ranges on the MiSeq i100 Series
        • Reagent Stability Guidelines for the MiSeq i100 Series Consumables
        • Recommended Network Security Allow List for the MiSeq i100 Series
        • Run Mode options for the MiSeq i100 Series
        • Sequencing Illumina Stranded Total RNA and mRNA Libraries on the MiSeq i100 Series
        • Specifications for the MiSeq i100 Series Instruments
        • Spiking custom primers into the Illumina sequencing primers for MiSeq i100 Series
        • Tested Product Guidance for USB External Storage Devices on the MiSeq i100 Series
        • Two Channel Chemistry and Imaging on the MiSeq i100 Series
        • Waste Volumes for MiSeq i100 Series Reagents
      • Troubleshooting
        • Cloud Analysis Errors using Sample Sheet Workflow on the MiSeq i100 Series
        • How to Troubleshoot Clustering Failures on the MiSeq i100 Series
        • Mitigating Instrument Platform Services Errors on the MiSeq i100 Series
        • Troubleshooting "Additional instrument configuration is required" Issues on the MiSeq i100 Series
        • Troubleshooting 'An unexpected error occurred during sequencing' Errors on the MiSeq i100 Series
        • Troubleshooting 'EdgeOS You are offline' errors on the MiSeq i100 Series
        • Troubleshooting 'Failed to start First Time Setup' Errors on the MiSeq i100 Series Instruments
        • Troubleshooting 'Status code 523' Errors in Cloud Settings on the MiSeq i100 Series
        • Troubleshooting APIException: Bad Request Errors when setting Default Output Folder on the MiSeq i100 Series
        • Troubleshooting CIM Homing Errors during Initialization on the MiSeq i100 Series
        • Troubleshooting Cloud Connectivity Pre Run Check Errors on the MiSeq i100 Series
        • Troubleshooting Flow Cell Thermal Errors during Pre Run Checks on the MiSeq i100 Series
        • Troubleshooting HttpClient.Timeout Errors during Pre Run Checks on the MiSeq i100 Series
        • Troubleshooting Inaccurate Estimated Completion Time on the MiSeq i100 Series
        • Troubleshooting Index Dropouts or No Reads Identified for Single Sample on the MiSeq i100 Series
        • Troubleshooting Insufficient Space on External Storage on the MiSeq i100 Series Instruments.
        • Troubleshooting Internal Server Error in Cloud Settings on the MiSeq i100 Series
        • Troubleshooting Issues with Waste Level Sensor System Check on the MiSeq i100 Series
        • Troubleshooting Leak Detection Errors on the MiSeq i100 Series
        • Troubleshooting Memory Metal Alloy (MMA) Motion Stalls on the MiSeq i100 Series
        • Troubleshooting Pressure Charging Failures on the MiSeq i100 Series
        • Troubleshooting Stalled Cloud Login Screen on the MiSeq i100 Series
        • Troubleshooting Stalled Secondary Analyses Onboard the MiSeq i100 Series.
        • Troubleshooting System Configuration errors during Pre Run Checks on the MiSeq i100 Series
        • Troubleshooting Touch Screen Not Responding on the MiSeq i100 Series
        • Troubleshooting Unable to connect to external storage Errors on the MiSeq i100 Series
    • NextSeq 500/550
      • FAQ
        • At which cycle of NextSeq 500/550 are run metrics calculated?
        • Can the NextSeq 500/550 v2.5 flow cell be used with the v2 Reagent cartridge and buffer cartridge?
        • Can the output folder for a NextSeq 500/550 run be saved to the local computer?
        • Common Questions Related to NextSeq 500/550 v2.5 Kit
        • Common questions regarding custom recipes for the NextSeq 500/550
        • Do NextSeq 500/550 v2.5 runs have different specifications than v2.0 reagent runs?
        • How long does a NextSeq 500/550 system check take to complete?
        • How long does it take to purge all reagents in a NextSeq 500/550 run?
        • How many extra cycles are included in NextSeq 500/550 reagent cartridges?
        • How to set up a NextSeq 500/550 to upload to a BaseSpace Enterprise account using NCS v.4.0.1
        • Run setup modes for the NextSeq 500/550
        • What do the NextSeq 500/550 status bar colors mean?
        • What is the difference between NextSeq PhiX Control Kit and PhiX Control v3?
        • What is the maximum read length supported in one direction on a NextSeq 500/550 run?
        • Will the NextSeq 500/550 Control Software prevent run setup with read lengths fewer than 25 cycles?
      • Reference Material
        • Best Practices for maintaining the computer on a NextSeq 500/550
        • Chemistry and imaging on NextSeq 500/550
        • Consumable part numbers for the NextSeq 500/550
        • File paths for the NextSeq 500/550
        • Frequently asked questions about upgrading to NextSeq 500/550 Control Software version 4.0
        • Hardware overview for the NextSeq 500/550
        • How to Denature and Dilute Libraries on the NextSeq 500/550 Video
        • How to Find the Control Software Version on the NextSeq 500/550
        • How to Perform a NextSeq 500/550 Power Cycle
        • How to Prepare Reagents Pre Run on the NextSeq 500/550 Video
        • How to Replace a NextSeq 500/550 Air Filter
        • How to change a run folder name on the NextSeq 500/550
        • How to create Local Run Manager credentials to perform a NextSeq 500/550 system check in NCS 4.0.X
        • How to denature and dilute libraries on the NextSeq 500/550
        • How to enable/disable Illumina Proactive on NextSeq 500/550
        • How to perform washes on the NextSeq 500/550
        • How to select custom recipes on the NextSeq 500/550 in NextSeq Control Software (NCS) 4.0.X
        • How to set up a PhiX validation run on the NextSeq 500/550 in manual mode
        • How to set up a Wash on the NextSeq 500/550 Video
        • How to set up a run on the NextSeq 500/550 Video
        • How to skip the pre run check confirmation on the NextSeq 500/550
        • How to turn on thumbnails for troubleshooting on the NextSeq 500/550
        • How to use a 10X Multiome ATAC Library custom recipe for NextSeq 500/550
        • Indexing considerations for NextSeq 500/550 sample sheets
        • Instructions for thawing and storing NextSeq 500/550 reagents
        • Layout for NextSeq 500/550 flow cells
        • Maximum read lengths and number of cycles for different NextSeq 500/550 kits
        • NextSeq 500/550 Control Software v4.0.1 "Invalid Role" error with Local Run Manager v2.4.0
        • NextSeq 500/550 Run Quality and Best Practices Support Webinar Video
        • Optical System on NextSeq 500/550
        • Post Run Automatic Purge for NextSeq 500/550
        • Reagent cartridge and buffer layout for NextSeq 500/550
        • Recommendations for using custom primers on the NextSeq 500/550
        • Run folder sizes and disk space on the NextSeq 500/550
        • Software information regarding the NCS 4.0.X upgrade for NextSeq 500/550
        • Specifications for the NextSeq 500/550
        • Temperature control on NextSeq 500/550
        • Thumbnail image information on the NextSeq 500/550
        • Troubleshooting "Would you like to include the first time setup results…?" on NextSeq 500/550
        • Upgrading to NextSeq Control Software v4
      • Troubleshooting
        • Best practices for NextSeq 500/550 pre run check failures requiring a power cycle
        • How to change a NextSeq 500/550 nickname
        • How to fix inaccurate or inverted touch screen on the NextSeq 500/550
        • How to perform a NextSeq 500/550 system check
        • Optimal cluster density guidelines for the NextSeq 500/550
        • Troubleshooting "Local Run Manager Error Row was updated or deleted" error on the NextSeq 500/550
        • Troubleshooting 'Lane Pump not initialized' fluidics related error on the NextSeq 500/550
        • Troubleshooting 'Not Found' error message on NextSeq 500/550
        • Troubleshooting Access to the path 'C:\NextSeqCalibration.cfg' is denied error on NextSeq 500/550
        • Troubleshooting Cannot Update Metrics errors on the NextSeq 500/550
        • Troubleshooting Custom Sequencing Primers selected by mistake in manual mode on the NextSeq 500/550
        • Troubleshooting Cycle 1 Error, Camera Disabled, Failed to Detect Clusters on the NextSeq 500/550
        • Troubleshooting Local Run Manager originally caused by an internal server error on NextSeq 500/550
        • Troubleshooting NVRAM or Socket Errors on NextSeq 500/550
        • Troubleshooting NextSeq 500/550 (NCS 2.2 or earlier) not showing Planned Run from BaseSpace PrepTab
        • Troubleshooting NextSeq 500/550 run stopping with "Local Run Manager Error: Bad Request" error
        • Troubleshooting Object Reference error on NextSeq 500/550
        • Troubleshooting Possible Stale Image Data Detected on the NextSeq 500/550
        • Troubleshooting RFID errors on NextSeq 500/550
        • Troubleshooting RheodyneValve error on NextSeq 500/550
        • Troubleshooting ThermoCouple_Side TemperatureProbe not operating as expected on the NextSeq 500/550
        • Troubleshooting Unable to Unload Reagents on the NextSeq 500/550
        • Troubleshooting Universal Copy Server error on the NextSeq 500/550 (NCS 4.0+)
        • Troubleshooting Wash State Unknown in NextSeq 500/550 Control Software (NCS) v4.0.0
        • Troubleshooting a CavroSyringePump Error on the NextSeq 500/550
        • Troubleshooting a Failed Camera Board on the NextSeq 500/550
        • Troubleshooting a Failed to Get Temperature error on the NextSeq 500/550
        • Troubleshooting a Flow Cell Door error on the NextSeq 500/550
        • Troubleshooting a Flow Cell Registration Failed (Skew) warning on the NextSeq 500/550
        • Troubleshooting a NextSeq 500/550 An existing connection was forcibly closed by a remote host error
        • Troubleshooting a NextSeq 500/550 Cavro Syringe Pump error
        • Troubleshooting a RAID error on the NextSeq 500/550
        • Troubleshooting a Remote Server Returned An Error on NextSeq 500/550
        • Troubleshooting a Software Shutdown Issue Warning on the NextSeq 500/550 with NCS 4.0.X
        • Troubleshooting a Specific Camera Disabled Failed to Detect Clusters error on the NextSeq 500/550
        • Troubleshooting a buffer cartridge/tray stuck in the NextSeq 500/550
        • Troubleshooting a flow cell registration stall during pre check on first run on NextSeq 500/550
        • Troubleshooting an Async Firmware Operation Error on the NextSeq 500/550
        • Troubleshooting an Internal Temperature was Out of Range error on the NextSeq 500/550
        • Troubleshooting an empty output folder on the NextSeq 500/550 NCS 4.0
        • Troubleshooting an error related to InstrumentMaintenanceState.xml on the NextSeq 500/550
        • Troubleshooting an imaging system failed pre run check error on the NextSeq 500/550
        • Troubleshooting an initialization failure due to Proxy warning on the NextSeq 500/550
        • Troubleshooting blank BaseSpace login screen during run setup for NextSeq 500/550
        • Troubleshooting flow cell registration failed to find fiducial on the NextSeq 500/550
        • Troubleshooting fluidics automation error on the NextSeq 500/550
        • Troubleshooting issues unloading reagents on a NextSeq 500/550
        • Troubleshooting mid run Camera Disabled error on the NextSeq 500/550
        • Troubleshooting no output folder created when set in the Local drive on NextSeq 500/550 (NCS 4.0)
        • Troubleshooting pre run checks with Temperature sensor check failed: Board 0/1 on NextSeq 500/550
        • Troubleshooting presence of snr.csv Files in Temp folder on NextSeq 500/550 (NCS 4.0.2)
        • Troubleshooting spent reagent cartridge is full on the NextSeq 500/550
        • Troubleshooting spent reagent cartridge not detected on NextSeq 500/550
    • NextSeq 1000/2000
      • FAQ
        • Can NextSeq 1000/2000 custom primers be diluted in in RSB with Tween instead of HT1?
        • Can NextSeq 1000/2000 run folders be set up to be deleted automatically?
        • Can a NextSeq 1000/2000 reagent cartridge be used if the foil packaging has been pierced?
        • Can a NextSeq 1000/2000 sequencing run be set up with fewer than 26 cycles in read 1?
        • Can a run be started on the NextSeq 1000/2000 without an active DRAGEN license?
        • Can a run on the NextSeq 1000/2000 use expired reagents?
        • Can additional users be added to the Linux interface on the NextSeq 1000/2000?
        • Can the NextSeq 1000/2000 run on 120V or 240V power?
        • Common Questions Related to Sequencing Analysis Viewer (SAV) software and the NextSeq 1000/2000
        • Custom recipes for Illumina Stranded libraries on NextSeq 1000/2000
        • Does the NextSeq 1000/2000 have thumbnail images available for troubleshooting?
        • Does the NextSeq 1000/2000 require a wash after run completes?
        • How to create new user accounts on the NextSeq 1000/2000
        • How to dispose of a NextSeq 1000/2000 flow cell?
        • How to obtain a NextSeq 1000/2000 v2 sample sheet?
        • How to switch user accounts on NextSeq 1000/2000
        • Is there a maximum number of cycles for index reads on the NextSeq 1000/2000?
        • What is the difference between RSB with Tween 20, part number 20047304 and 20050639?
        • Which NextSeq 1000/2000 run modes require a v2 sample sheet?
      • Reference Material
        • Air filter replacement on the NextSeq 1000/2000
        • Autocenter process and images on the NextSeq 1000/2000
        • Best practices for custom sequencing primer use on the NextSeq 1000/2000
        • Chemistry and imaging on the NextSeq 1000/2000
        • Cleaning NextSeq 1000/2000 cartridge tray in case of reagent leak inside the instrument
        • Consumable part numbers for the NextSeq 1000/2000
        • Custom recipes on the NextSeq 1000/2000
        • Demultiplex information from DRAGEN on the NextSeq 1000/2000
        • Disk Management on the NextSeq 1000/2000
        • Editing runs from Run Planning after selection in the NextSeq 1000/2000 control software
        • End of Life Schedule for NextSeq 1000 / 2000 SBS Standard Consumables
        • File paths on the NextSeq 1000/2000
        • Flow cell overview for the NextSeq 1000/2000
        • How To Perform a System Check on the NextSeq 1000/2000 Video
        • How To Replace the NextSeq 1000/2000 Air Filter Video
        • How To Update The Software On A NextSeq 1000/2000 Video
        • How to Manually Start and Stop Universal Copy Service (UCS) on the NextSeq 1000/2000 Video
        • How to Mount a Network Drive via Network File System (NFS) on NextSeq 1000/2000 Video
        • How to Mount a Network Drive via SMB/CIFS on NextSeq 1000/2000 Video
        • How to Use the Cartridge Recycling Tool for NextSeq 1000/2000 Video
        • How to check free disk space via terminal on the NextSeq 1000/2000
        • How to install TeamViewer QuickSupport on NextSeq 1000/2000
        • How to navigate to TeamViewer on the NextSeq 1000/2000
        • How to perform Linux OS updates on the NextSeq 1000/2000
        • How to power cycle the NextSeq 1000/2000
        • How to properly delete runs on the NextSeq 1000/2000 Video
        • How to requeue a NextSeq 1000/2000 run locally (on the instrument)
        • How to setup a sequencing run for the NextSeq 1000/2000
        • Index color balancing for the NextSeq 1000/2000 system using standard SBS reagents
        • Index orientation in v2 sample sheets for the NextSeq 1000/2000
        • Index read strategy for the NextSeq 1000/2000
        • Introducing the NextSeq 1000/2000 P1 100 cycle, P1 600 cycle and P2 600 cycle reagent kits
        • Introducing the NextSeq 1000/2000 P1 Reagent kit
        • Introduction to the NextSeq 1000/2000 System Support Webinar Video
        • Library denaturation for the NextSeq 1000/2000
        • Links to resources and helpful notes for the NextSeq 1000/2000
        • Lost connection with server error on the NextSeq 1000/2000
        • Manual denaturation of libraries on the NextSeq 1000/2000
        • Moving custom recipes using the Linux terminal on the NextSeq 1000/2000
        • Networking and output locations on the NextSeq 1000/2000
        • NextSeq 1000/2000 Control Software v1.5.0 installation instructions
        • NextSeq 1000/2000 Loading Optimization for XLEAP SBS kits
        • NextSeq 1000/2000 XLEAP SBS Cartridge Preparation Video
        • NextSeq 1000/2000 loading optimization for Standard SBS kits
        • NextSeq 1000/2000 reagents disposal and cartridge recycling tool
        • Purge settings on the NextSeq 1000/2000
        • Real Time Analysis (RTA) on NextSeq 1000/2000 Overview
        • Reference images for NextSeq 1000/2000
        • Returning NextSeq 1000/2000 reagents to storage
        • Run mode options for the NextSeq 1000/2000
        • Sample sheets for the NextSeq 1000/2000
        • Setting external hard drives as the default output folder on NextSeq 1000/2000
        • Shelf life of NextSeq 1000/2000 reagents
        • Specifications for the NextSeq 1000/2000
        • Storage temperature, shipping conditions and dimensions of NextSeq 1000/2000 reagents
        • System Checks for the NextSeq 1000/2000
        • Thawing and reagent preparation guidelines for the NextSeq 1000/2000 reagents
        • Tips and tricks for navigating the Linux Environment on the NextSeq 1000/2000
        • User accounts and password management on the NextSeq 1000/2000
        • Viewing NextSeq 1000/2000 runs in Sequencing Analysis Viewer
        • What does NextSeq 1000/2000 status bar color mean?
      • Troubleshooting
        • Failure to find fiducial errors on the NextSeq 1000/2000
        • Flow cell is not detected error on the NextSeq 1000/2000
        • How To Power Cycle NextSeq 1000/2000 Video
        • How to change passwords and unlock accounts on the NextSeq 1000/2000
        • How to resolve an error occurred while populating NextSeq 1000/2000 data in SAV
        • Humidity warning during pre run check on the NextSeq 1000/2000
        • Internal network issues on the NextSeq 1000/2000
        • Manually stopping and restarting Universal Copy Service (UCS) on the NextSeq 1000/2000
        • NextSeq 1000 and 2000 Run Quality and Best Practices Support Webinar Video
        • NextSeq 1000/2000 Control Software upgrade from 1.7.0 to 1.7.1 system integration error
        • Pre Run check failed with an alignment check failure on the NextSeq 1000/2000
        • Pre run check failed at Fluidics stage on the NextSeq 1000/2000
        • Recommendations for using external hard drives on the NextSeq 1000/2000 platforms
        • Resolving issues with Process Management on NextSeq 1000/2000
        • Sample Sheet Import Errors on the NextSeq 1000/2000
        • Single tile drop outs affecting metrics on the NextSeq 1000/2000
        • Troubleshooting "An error occurred while checking requeue availability" on the NextSeq 1000/2000
        • Troubleshooting "Error performing Sequencing_ProtocolName_Safestate" error on the NextSeq 1000/2000
        • Troubleshooting "Failed to send WATCHDOG=1 notification message:" on the NextSeq 1000/2000
        • Troubleshooting 'mount' Errors when Networking NextSeq 1000/2000 Instruments
        • Troubleshooting DRAGEN version warnings and errors after the NextSeq 1000/2000 Control Software upgrade to version 1.7
        • Troubleshooting Failed to execute connectivity check input parameters on the NextSeq 1000/2000
        • Troubleshooting Filesystem type exFAT not configured in kernel message on the NextSeq 1000/2000
        • Troubleshooting NextSeq 1000/2000 Control Software failure to initialize with cartridge error
        • Troubleshooting NextSeq 1000/2000 eject cartridge prompt remains after ejecting the cartridge
        • Troubleshooting NextSeq 1000/2000 low read 1 intensity
        • Troubleshooting System File errors during pre run checks on the NextSeq 1000/2000
        • Troubleshooting a black screen after a power cycle on the NextSeq 1000/2000
        • Troubleshooting install package integrity check failed during NextSeq 1000/2000 upgrade to v1.7
        • Troubleshooting keyring prompts on the NextSeq 1000/2000
        • Troubleshooting network output directory issues after updating to NextSeq 1000/2000 CS v1.5
        • Troubleshooting no DRAGEN License after installing DRAGEN BCL Convert 4.2
        • Understanding loading and percent loading concentration on the NextSeq 1000/2000
        • Universal Copy Service failed to validate the Output Folder error on the NextSeq 1000/2000
        • Updating the system time on the NextSeq 1000/2000
        • Why is NextSeq 1000/2000 control software update not available from the Software Update menu?
    • cBot
      • Reference Material
        • How to perform a Read 1 rehybridization of a HiSeq v4 high output flow cell on the cBot
    • HiSeq 1000/2500
      • Reference Material
        • HiSeq 1000/1500/2000/2500 rehybridization quick reference chart
        • How to quick format data drives on HiSeq 1000/1500/2000/2500
    • HiSeq X
      • Reference Material
        • Technical resources for the HiSeq X system
    • NovaSeq X/X Plus
      • FAQ
        • Custom recipes for Illumina Stranded libraries on NovaSeq X Series
        • Does the NovaSeq X/X Plus use Single or Multi Node Connection Terminations?
        • Effects of library quality and indexes on demultiplexing NovaSeq X Series data
        • Handling and Disposal Recommendations for Used Reagents on the NovaSeq X Series
        • When are the run folders created after initiating a sequencing run on the NovaSeq X/X Plus?
        • Which lanes should be selected during sample sheet creation for a 1.5B run on the NovaSeq X/X Plus
      • Reference Material
        • Air filter replacement on the NovaSeq X Series
        • Chemistry and Imaging on the NovaSeq X Series Instruments
        • Consumable catalog numbers for the NovaSeq X Series kits
        • File paths on the NovaSeq X Series
        • How to Power Cycle the NovaSeq X Series Instruments
        • How to Restart File Transfer on the NovaSeq X Series
        • How to access network storage locations in Linux on the NovaSeq X Series
        • How to add custom index adapter and library prep kits to the NovaSeq X Series Instruments
        • How to add new NovaSeq X Series Control Software users on the NovaSeq X/X Plus
        • How to change passwords for the Control Software accounts on the NovaSeq X Series Instruments
        • How to change the Linux OS passwords on the NovaSeq X Series Instruments
        • How to configure External Storage locations on the NovaSeq X Series
        • How to install TeamViewer QuickSupport on the NovaSeq X Series Instruments
        • How to perform a Maintenance Wash on the NovaSeq X Series Instruments
        • How to plan a Local sequencing run on the NovaSeq X Series
        • How to use custom primers on the NovaSeq X Series
        • How to use the Log Bundler v1.3 Application on NovaSeq X Series
        • Illumina specifications for the NovaSeq X Series
        • Index color balancing for XLEAP SBS reagents on the NextSeq 1000/2000 and NovaSeq X/X Plus
        • Introducing the Illumina NovaSeq X System
        • Library Loading Concentration and Optimization Guide for NovaSeq X/X Plus Instruments
        • Library loading concentrations and considerations for the NovaSeq X/X Plus instruments
        • Optimizing Library Insert Size Representation on NovaSeq X Series Instruments
        • PhiX Spike In Requirements for Low Diversity Libraries on NovaSeq X Series Instruments
        • Planning a Manual Mode Run on NovaSeq X/X Plus
        • RTA4 Q Score Bin Ranges on the NovaSeq X/X Plus
        • Reagent stability and thawing guidelines for the NovaSeq X Series
        • Run Mode options for the NovaSeq X Series
        • Troubleshooting Insufficient Storage Space Errors during Pre Run Checks on the NovaSeq X Series
        • User Accounts and Password Management on the NovaSeq X/X Plus Instrument
        • Using expired reagents on the NovaSeq X Series
        • Using the BaseSpace Command Line Interface (CLI) Tool on the NovaSeq X Series
        • Validated USB Storage Devices for NovaSeq X Series Control Software v1.3.1
        • Wash Flow Cell Use Counter on NovaSeq X Series
      • Troubleshooting
        • How to Resolve "sma_air_a/b was not present in dictionary" Errors During Pre Run Checks on the NovaSeq X Plus
        • How to Troubleshoot No Intensity or 0% Passing Filter at Inlet and Outlet Tiles on NovaSeq X Series
        • How to requeue or perform a new analysis of existing data on the NovaSeq X Series
        • How to resolve 'An unknown error has occurred' critical system errors on the NovaSeq X Series Instruments
        • How to resolve 'Critical System Error: An unexpected error has occurred with system integration' during initialization on the NovaSeq X/X Plus
        • How to resolve Network Connection warnings during Pre Run Checks on the NovaSeq X Series
        • How to resolve issues with keyboard tray ejecting on the NovaSeq X/X Plus
        • How to troubleshoot "The instrument encountered a thermal coolant error" on the NovaSeq X Series
        • How to troubleshoot CIB Fault Initialization errors on the NovaSeq X/X Plus
        • How to troubleshoot Insufficient Memory errors during Pre Run Checks on the NovaSeq X Series Instruments
        • How to troubleshoot MaxWetS2SpotIntensity Errors during Wet Pre Run Checks on the NovaSeq X Series
        • How to troubleshoot clustering failures on the NovaSeq X Series
        • How to troubleshoot errors during the pre run Wet Checks on the NovaSeq X/X Plus
        • How to troubleshoot low percent clusters passing filter (%PF) on the NovaSeq X/X Plus
        • Troubleshooting "Unable to update index information of the Library" on the NovaSeq X/X Plus
        • Troubleshooting 'A configuration file cannot be assessed' Warnings during Pre Run Checks on the NovaSeq X Series
        • Troubleshooting 'Insufficient memory to continue the execution of the program' errors on the NovaSeq X Plus
        • Troubleshooting 'One or more unknown settings found' Errors during Run Planning on the NovaSeq X Series
        • Troubleshooting Flow Cell Vacuum Failed to Engage on the NovaSeq X/X Plus
        • Troubleshooting Issues with Allocating Disk Space during Pre Run Checks on the NovaSeq X Series
        • Troubleshooting Reagent Compartment Doors Failing to Unlock on the NovaSeq X Series
        • Troubleshooting Regions of Reduced %Passing Filter on NovaSeq X Series
        • Troubleshooting multiple entries with identical indices error during run setup on NovaSeq X Series
    • NovaSeq 6000
      • FAQ
        • Can a NovaSeq 6000 4 lane Xp kit be used for 2 lane NovaSeq 6000 flow cells?
        • Can a different tube type be used in place of the library tubes for NovaSeq 6000 runs?
        • Can a lab network be upgraded to support faster file transfer on the NovaSeq 6000?
        • Can custom primers be used on the NovaSeq 6000 System?
        • Can different libraries pools be loaded in each lane when using NovaSeq 6000 Xp workflow?
        • Can expired consumables be used for a NovaSeq 6000 run?
        • How does the NovaSeq 6000 Xp workflow differ from NovaSeq 6000 standard workflow?
        • How long does it take for Universal Copy Service copying to complete on NovaSeq 6000?
        • How long will the NovaSeq 6000 UPS support the instrument should backup power not engage?
        • How often does the NovaSeq 6000 wash flow cell need to be replaced?
        • Is a local copy of each run saved on the NovaSeq 6000?
        • What do NovaSeq 6000 status bar colors mean?
        • What effect does prolonged flow cell staging time have on clustering for the NovaSeq 6000?
        • What is the library loading concentration for NovaSeq 6000 Xp workflow?
        • What volume of ExAmp reagent is added by the NovaSeq 6000 during a standard run?
        • When can runs be staggered on NovaSeq 6000?
        • Where to find the Safety Data Sheet (SDS) for NovaSeq6000 coolants?
        • Which types of sample sheet can be used when analyzing NovaSeq 6000 run data?
      • Reference Material
        • Best practices when updating to NovaSeq 6000 Control Software v1.8
        • Computer Hardware Specifications for NovaSeq 6000
        • Consumable catalog numbers for the NovaSeq 6000 v1.5
        • How To Power Cycle NovaSeq 6000 Video
        • How to Enable/Disable Illumina Proactive on the NovaSeq 6000
        • How to Perform a System Health Check on the NovaSeq 6000 Video
        • How to Power Cycle the NovaSeq 6000 System
        • How to configure a NovaSeq 6000, with Control Software 1.8.x or greater, to work with a Proxy Server
        • How to perform system checks on the NovaSeq 6000
        • How to set the output location in the NovaSeq 6000 Control Software
        • How to set up a PhiX validation run on the NovaSeq 6000 sequencing platform
        • How to start a maintenance wash without used buffer cartridges on the NovaSeq 6000
        • How to stop and restart Universal Copy Service manually on the NovaSeq 6000
        • Index color balancing for the NovaSeq 6000 system
        • Introducing NovaSeq Control Software version 1.8
        • Introducing the NovaSeq 6000 v1.5 reagents
        • Minimum storage requirements to start a NovaSeq 6000 run
        • NovaSeq 6000 File paths
        • NovaSeq 6000 Introduction Support Webinar Video
        • NovaSeq 6000 flow cell types and their compatibility with NovaSeq Control Software versions
        • NovaSeq 6000 storage management and best practices
        • Reagent Cartridge Layout for NovaSeq 6000
        • Real Time Analysis (RTA3) on NovaSeq 6000 Overview
        • Reference images for NovaSeq 6000
        • Software Restriction Policy (SRP) on the NovaSeq 6000
        • Specifications for the NovaSeq 6000
        • Thawing, stability, and storage of NovaSeq 6000 reagents
        • Troubleshooting Focus Camera (FTM) system check failure on the NovaSeq 6000
        • Washing the NovaSeq 6000
        • What to do if the NovaSeq 6000 flow cell cannot be loaded during run setup?
        • Where are NovaSeq 6000 system check results located?
        • Xp reagents components and stability on the NovaSeq 6000
        • 测序前准备和检查NovaSeq 6000 流动槽的最佳操作
      • Troubleshooting
        • Best practices for preparing and inspecting NovaSeq 6000 flow cells for sequencing
        • Best practices for starting staggered runs on the NovaSeq 6000
        • How to troubleshoot D:\ drive full on the NovaSeq 6000
        • How to troubleshoot NovaSeq 6000 Process Management errors
        • How to troubleshoot NovaSeq 6000 light bar issues
        • How to troubleshoot NovaSeq 6000 “A component failed during sequencing” error during a Cloud Outage
        • How to troubleshoot Reagent Selector Valve Errors on the NovaSeq 6000
        • How to troubleshoot failed to reach target temperature error on the NovaSeq 6000
        • How to troubleshoot flow cell clamp or vacuum is unable to engage errors when loading flow cells on the NovaSeq 6000
        • How to troubleshoot “A component failed during sequencing” error messages on the NovaSeq 6000
        • NovaSeq 6000 Initialization failed at 93% (NVCS 1.7) or 92% (NVCS 1.5, 1.6)
        • NovaSeq 6000 Run Quality and Best Practices Support Webinar Video
        • Run listed as Uploading to Network or BaseSpace on the NovaSeq 6000
        • Troubleshooting "Component Failed" errors immediately after Pre Run Checks on the NovaSeq 6000
        • Troubleshooting "Instrument Shell has Stopped Working" on the NovaSeq 6000
        • Troubleshooting "Unsupported Cluster Cartridge" warning during Run Setup on the NovaSeq 6000
        • Troubleshooting "flow cell is not sufficiently level" error on the NovaSeq 6000
        • Troubleshooting "ix_exception_id_errno_exist: 'Already Exists' on command" on the NovaSeq 6000
        • Troubleshooting BIM SOUR 2 Handle Position Errors During NovaSeq 6000 System Checks
        • Troubleshooting Black Screen with EFI Shell message on the NovaSeq 6000
        • Troubleshooting CE or Z:\ drive not connecting on the NovaSeq 6000
        • Troubleshooting Fatal error, exiting application. Unable to find device on the NovaSeq 6000
        • Troubleshooting Network errors during run setup on the NovaSeq 6000
        • Troubleshooting NovaSeq 6000 Control Software fails to initialize with Universal Copy Service error
        • Troubleshooting NovaSeq 6000 Reagent Chiller outside acceptable range warning
        • Troubleshooting NovaSeq 6000 failing to initialize control software at 94 95%
        • Troubleshooting NovaSeq 6000 flow cell loading issues Video
        • Troubleshooting NovaSeq 6000 optical alignment errors, BIRD warnings, and Objective Contamination
        • Troubleshooting NovaSeq 6000 reagent RFID failures
        • Troubleshooting Pressure decay or Impedance test stopped with NaN results on the NovaSeq 6000
        • Troubleshooting SAV errors on the NovaSeq 6000
        • Troubleshooting Unable to Start Real Time Analysis on the NovaSeq 6000
        • Troubleshooting an Unable to Generate a Focus Model Error on the NovaSeq 6000
        • Troubleshooting decreased intensity and clusters passing filter at flow cell inlet on NovaSeq 6000
        • Troubleshooting laser coolant temperature errors on the NovaSeq 6000
        • Troubleshooting pre run check errors on the NovaSeq 6000
        • Troubleshooting the "Unable to load recipe" error on the NovaSeq 6000
        • Troubleshooting the large used reagent bottle is full error on the NovaSeq 6000
        • Troubleshooting vacuum failures and flow cell vacuum is no longer engaged message on NovaSeq 6000
        • Troubleshooting “Unable to Register Fiducial” error on the NovaSeq 6000
        • Using expired reagents on the NovaSeq 6000
    • Instrument Administration
      • Reference Material
        • How to add an Administrator account to Windows 10 Operating System on the NovaSeq 6000
        • How to cache credentials for access to Network Storage and verify Read/Write access
        • How to change the Log On As account for the Illumina Universal Copy Service
        • How to change the Windows account type from administrator to standard
        • How to check if a Windows account is a standard or administrator account
        • How to confirm firewall settings and close port 29644 on NovaSeq 6000
        • How to install Windows critical security updates
        • How to switch user accounts on Illumina Windows 10 Sequencing System
      • Troubleshooting
        • Troubleshooting 'Installation failure. Check installed software and try again.' error
        • Troubleshooting Local Run Manager Notification ‘Universal Copy Service does not use the same user’
        • Troubleshooting Windows services menu shows Log On As options greyed out
  • Library Preparation
    • General
      • FAQ
        • Can Illumina RNA Prep with Enrichment library prep and enrichment reagents be purchased separately?
        • Can PhiX and denatured PhiX be stored and used again?
        • Can RSB be used as a substitute for RSB + Tween 20 when sequencing on the NextSeq 1000/2000?
        • Can library preparation reagents be stored in a frost free freezer?
        • Can samples be sent to Illumina for sequencing; does Illumina offer sequencing as a service?
        • Can wells in index plates be used more than once?
        • Catalog/Reference numbers for IDT for Illumina DNA/RNA UD and Illumina DNA/RNA UD Indexes
        • Denature and dilution FAQ for Illumina DNA PCR Free
        • Does library loading differ between MiSeq Micro and Nano flow cells?
        • How much PhiX to spike in for well balanced, base diverse libraries?
        • How to perform library heat denaturation (heat shock) before sequencing?
        • How to prepare < 2 nM libraries for sequencing on NovaSeq X/X Plus
        • Hybridization Buffer (HT1) and Resuspension Buffer (RSB) alternatives and purchasing availability
        • IDT for Illumina TruSeq Unique Dual (UD) Index kit FAQs for kit configurations and usage
        • Indexed Sequencing workflows with 5' to 3' directionality
        • Information about the TruSeq index adapter plate fixture
        • Introducing the Illumina DNA/RNA UD Indexes
        • Link to the Sequencing Methods Explorer
        • Plate layout for the Illumina DNA/RNA UD Indexes (v3)
        • Product page and support page links for Illumina DNA Prep with Enrichment (S) Tagmentation
        • Product page and support page links for Illumina RNA Prep with Enrichment, (L) Tagmentation
        • Product page and support page links for TruSeq DNA Exome
        • Product page and support page links for TruSeq Methyl Capture EPIC
        • Product page and support page links for TruSight Cancer bundled with TruSight Rapid Capture
        • Product page and support page links for TruSight Myeloid
        • Should index reads be performed if only one library is being sequenced?
        • Should indexes be read when sequencing a single library?
        • What are the Illumina sequencing primer sequences?
        • What are the index sequences for the AmpliSeq CD Indexes Large Volume kit for Illumina?
        • What do (S), (M), and (L) mean in Illumina library prep kit names?
        • What is a Single Read (SR), Paired End (PE) reads, and read requirements for sequencing?
        • What is the concentration of Illumina adapters and primers?
        • What is the difference between a single and dual indexed library?
        • What is the minimum library size that can be sequenced?
        • Where are the sequences and plate positions of Illumina indexes?
        • Where to find the reference sequence used for phiX alignment?
        • Which TruSeq index kits come in plate or tube format?
        • Which computer operating systems are compatible with Illumina Experiment Manager (IEM)?
      • Reference Material
        • Adapter dimers的形成原因、影响及去除方法
        • Amplicon Sequencing Introduction Support Webinar Video
        • Bead handling best practices
        • Bead types in Illumina library preparation kits
        • Best practices for library quantification
        • Best practices for manually normalizing library concentrations
        • Best practices for using Sodium Hydroxide with Illumina sequencing systems
        • Bubble products in sequencing libraries: causes, identification, and workflow recommendations
        • Choosing a Library Loading Concentration for Illumina Sequencing Video
        • Compatible and recommended Illumina library types for the NovaSeq 6000 sequencing platform
        • Considerations when migrating non Illumina libraries between sequencing platforms
        • DNA/RNA isolation considerations when using Illumina library prep kits
        • Final library trace examples for the Illumina Library Prep Kits
        • Fusion Genes kits for detection and lists of targeted genes
        • How Can I Tell If I Sequenced Through the Insert? Part 1 Video
        • How Can I Tell If I Sequenced Through the Insert? Part 2 Video
        • How Do I Achieve Consistent Quantitation? Part 1 Video
        • How Do I Achieve Consistent Quantitation? Part 2 Video
        • How Do I Optimize Amplicon Sequencing Data? Part 1 Video
        • How Do I Optimize Amplicon Sequencing Data? Part 2 Video
        • How short inserts affect sequencing performance
        • How to Plan Your First Sequencing Project Support Webinar Video
        • How to Use the Illumina Library Prep Kit Selector Video
        • How to find a Safety Data Sheet
        • How to mitigate index hopping
        • How to see more content and products relevant to your field of interest
        • How to use the Illumina Sequencing Coverage Calculator Video
        • IDT for Illumina DNA/RNA Unique Dual Index Compatibility on the MiniSeq
        • Illumina library prep kits and associated index kits
        • Illumina 接头系列
        • Illumina建库试剂盒和对应的index试剂盒
        • Index content for the TruSeq DNA and TruSeq RNA Single Index kits, Sets A and B
        • Indexed Sequencing Overview for Paired End Flow Cells
        • Introduction to Illumina DesignStudio Support Webinar Video
        • Is it possible to pool different library types in the same sequencing run?
        • Library quantification and quality control quick reference guide
        • Metagenomics Part I Introduction to Library Preparation and Sequencing Support Webinar Video
        • Optimal Cluster Density Best Practices Video
        • PhiX Control v3文库是什么,它在Illumina下一代测序中起到什么作用?
        • Plate layout for TruSeq Combinatorial Dual (CD) indexes
        • Plate layout for TruSeq Unique Dual (UD) indexes
        • Plate layouts for IDT for Illumina DNA/RNA UD Index kits, Sets A, B, C, and D
        • Recommended quality control of FFPE samples for Illumina FFPE supported library preparation kits
        • Resources for getting started with Illumina Next Generation Sequencing (NGS)
        • Sequencing FFPE Samples Introduction Support Webinar Video
        • Sequencing Method Explorer:哪种二代测序(NGS)方法最适合我的研究?
        • Sequencing primer compatibility and recommended library kits for the HiSeq 1000/2000 and 1500/2500
        • Sequencing primer compatibility and recommended library kits for the NextSeq 1000/2000 platforms
        • Sequencing primer compatibility and recommended library types for the HiSeq X and HiSeq 3000/4000
        • Sequencing primer compatibility of Illumina libraries and kit types for NextSeq 500/550 and MiniSeq
        • Sequencing primer compatibility of Illumina libraries and library types for the iSeq 100
        • Sequencing primer compatibility of Illumina libraries and recommended library kit for the MiSeq
        • Spiking custom primers into the Illumina sequencing primers
        • Understanding the differences between Illumina index chemistries and anchor function
        • Understanding unique dual indexes (UDI) and associated library prep kits
        • Using Sequencing Method Explorer to identify suitable NGS methods for research
        • What is the PhiX Control v3 Library and what is its function in Illumina Next Generation Sequencing
        • What sequences do I use for adapter trimming
        • Why pooling libraries at the same concentration does not change the final pool concentration
        • 了解唯一双标签(UDI)与相关建库试剂盒
        • 将定制引物掺入到Illumina测序引物中
        • 手动文库浓度标准化最佳操作
        • 文库定量与质量控制简明指引
        • 是否可以混合不同类型的文库并同时进行测序?
        • 最小化 TruSeq Stranded Total RNA 文库中rRNA污染的最佳操作
        • 末端接头剪切(adapter trimming)时需要用到的序列
      • Troubleshooting
        • Adapter dimers causes, effects, and how to remove them
        • Library QC and Troubleshooting with the Bioanalyzer and Fragment Analyzer Support Webinar Video
        • Preventing Contamination Expert Video Tip Video
        • Troubleshooting elevated PhiX alignment in sequencing runs
    • AmpliSeq
      • FAQ
        • Frequently asked questions for AmpliSeq for Illumina On Demand panels
        • How many reactions are in the AmpliSeq Library Equalizer for Illumina?
        • What is the difference between AmpliSeq for Illumina custom DNA HotSpot and Gene Design Panels?
      • Reference Material
        • AmpliSeq Antimicrobial Resistance Research Panel product, support, and manifest page links
        • AmpliSeq Custom DNA Panel product and support pages
        • AmpliSeq Custom RNA Panel product and support pages
        • AmpliSeq Inborn Errors of Metabolism Research Panel v2 product, support, and manifest page links
        • AmpliSeq On Demand Panel Product and Support Pages
        • AmpliSeq for Illumina Autism Research Panel product page, support page, and manifest links
        • AmpliSeq for Illumina BRCA Panel product page and support page links
        • AmpliSeq for Illumina BRCA Plus, Extended Hereditary Breast and Ovarian Research Panel page links
        • AmpliSeq for Illumina Cancer Hotspot v2 Panel product page and support page links
        • AmpliSeq for Illumina Cardiovascular Research Panel product page, support page, and manifest links
        • AmpliSeq for Illumina Childhood Cancer Panel product page and support page links
        • AmpliSeq for Illumina Colon and Lung Research Panel v2 product, support, and manifest page links
        • AmpliSeq for Illumina Comprehensive Panel v3 product page and support page links
        • AmpliSeq for Illumina Deafness Research Panel v2 product page, support page, and manifest links
        • AmpliSeq for Illumina Dementia Research Gene Panel product page, support page, and manifest links
        • AmpliSeq for Illumina Dermatology Research Panel v2 product page, support page, and manifest links
        • AmpliSeq for Illumina Dysmorphia Dysplasia Research Panel v2 product, support, & manifest page links
        • AmpliSeq for Illumina Ebola Research Panel product page, support page, and manifest links
        • AmpliSeq for Illumina Focus Panel product page and support page links
        • AmpliSeq for Illumina Gastrointestinal Research Panel v2 product, support, and manifest page links
        • AmpliSeq for Illumina Hearing Loss Research Panel v1 product page, support page, and manifest links
        • AmpliSeq for Illumina Hematology Research Panel product page, support page, and manifest links
        • AmpliSeq for Illumina Immune Repertoire Panel Plus, TCR beta Panel product and support page links
        • AmpliSeq for Illumina Immune Response Panel product page and support page links
        • AmpliSeq for Illumina Inherited Cancer Research Panel product page, support page, and manifest links
        • AmpliSeq for Illumina Introduction Support Webinar Video
        • AmpliSeq for Illumina Library Prep, Indexes, and Accessories product page and support page links
        • AmpliSeq for Illumina Long Non Coding RNA Research Panel product, support, and manifest page links
        • AmpliSeq for Illumina Myeloid Panel product page and support page links
        • AmpliSeq for Illumina Neurological Research Panel product page, support page, and manifest links
        • AmpliSeq for Illumina Noonan Research Panel product page, support page, and manifest links
        • AmpliSeq for Illumina Ophthalmic Research Panel product page, support page, and manifest links
        • AmpliSeq for Illumina Primary Immune Deficiency Research Panel v2 page links
        • AmpliSeq for Illumina Pulmonary Research Panel v2 product page, support page, and manifest links
        • AmpliSeq for Illumina RNA Fusion Lung Cancer Research Panel product, support, & manifest page links
        • AmpliSeq for Illumina RNA Inflammation Response Research Panel page links
        • AmpliSeq for Illumina RNA MAPK Pathway Research Panel product page, support page, and manifest links
        • AmpliSeq for Illumina RNA Stem Cell Research Panel product page, support page, and manifest links
        • AmpliSeq for Illumina RNA WNT Signaling Pathway Research Panel page links
        • AmpliSeq for Illumina Renal Research Panel v2 product page, support page, and manifest links
        • AmpliSeq for Illumina SARS CoV 2 Research Panel product page, support page, and manifest links
        • AmpliSeq for Illumina TB Research Panel product page, support page, and manifest links
        • AmpliSeq for Illumina TCR beta SR Panel product page and support page links
        • AmpliSeq for Illumina Transcriptome Panel product page and support page links
        • Number of libraries per AmpliSeq for Illumina Library PLUS kit for custom and community panels
        • Number of reactions per AmpliSeq for Illumina custom spike in panel order
        • Number of tubes in an AmpliSeq Custom or Community Panel orders
        • Performing a spike in of custom oligos into an AmpliSeq for Illumina Panel
        • Plate layout for AmpliSeq Unique Dual (UD) indexes
        • Plate layouts for Ampliseq Combinatorial Dual (CD) Indexes Sets A, B, C and D
        • Pooling recommendations for the AmpliSeq for Illumina Comprehensive Panel v3
        • Pooling recommendations for the AmpliSeq for Illumina Focus Panel
        • Pooling recommendations for the AmpliSeq for Illumina Myeloid Panel
    • Amplicon Library Prep
      • FAQ
        • Can IDT for Illumina DNA/RNA UD Indexes be used with the Illumina 16S Metagenomics Protocol?
        • Information and protocol for the Illumina 16S Metagenomics Sequencing Library Preparation workflow
        • Information and protocol for the Illumina Fungal Metagenomics Sequencing workflow
        • Overview of the Fungal Metagenomic Sequencing Demonstrated Protocol
      • Reference Material
        • Amplicon Sequencing Assessing the Success of Your Run Support Webinar Video
        • How to use Illumina DesignStudio for Custom Sequencing Panels Video
        • Recommendations for sequencing Illumina 16S libraries with the NextSeq 1000/2000 600 cycle kits
        • What Does a Typical 16S Run Look Like on a MiSeq?
    • Custom Third party Library Prep
      • FAQ
        • Are the NextSeq 1000/2000 Standard SBS Read & Index Primer kits compatible XLEAP SBS Reagents?
        • How do stagger sequences or heterogeneity spacers introduce diversity?
    • DNA Library Prep
      • FAQ
        • Analysis FAQ for Illumina DNA PCR Free libraries
        • BLT PF handling for the Illumina DNA PCR Free workflow questions
        • BLT PF usage FAQ for Illumina DNA PCR Free
        • Bead clean up ratios used in the Illumina 16S demonstrated protocol
        • Bead size selection FAQ for Illumina DNA PCR Free
        • Can FFPE input be used with the Illumina DNA PCR Free library preparation kit?
        • Consumables used in Illumina DNA PCR Free workflow
        • Differences between Illumina DNA Prep (M) Tagmentation (Nextera DNA Flex), Nextera DNA, and Nextera XT kits
        • Does the Flex Lysis Reagent Kit require the purchase of additional Illumina reagents?
        • How does the Illumina/Nextera tagmentation enzyme make sure that both p5 and p7 adapters are added?
        • How to quantify and what is the expected yield for Illumina DNA PCR Free libraries?
        • Illumina DNA PCR Free custom sequencing primer FAQ
        • Index selection and FAQ for Illumina DNA PCR Free
        • Indexing FAQ for Illumina DNA PCR Free libraries
        • Input quantity for Illumina DNA PCR Free libraries
        • Input types FAQ for Illumina DNA PCR Free libraries
        • Instrument, flow cell, and reagent requirement FAQ for Illumina DNA PCR Free libraries
        • Is the Nextera TDE1 enzyme pre loaded with oligos?
        • Library storage FAQ for Illumina DNA PCR Free libraries
        • Product page and support page for Illumina DNA Prep, (M) Tagmentation
        • Product page and support page links for Illumina DNA PCR Free
        • Product page and support page links for Nextera XT
        • Product page and support page links for TruSeq ChIP
        • Qualification/QC FAQ for Illumina DNA PCR Free libraries
        • Saliva collection for Illumina DNA PCR Free library preparation questions
        • Saliva processing for Illumina DNA PCR Free library preparation workflow questions
        • Sample Input for Illumina DNA Prep, (M) Tagmentation Library Preparation Kit
        • Sample batching with the Illumina DNA PCR Free kit questions
        • Samples per sequencing run and coverage FAQ for Illumina DNA PCR Free
        • Sequencing run setup software considerations FAQ for Illumina DNA PCR Free libraries
        • Single or double sided bead clean ups with Illumina DNA PCR Free.
        • Size selection and clean up considerations for TruSeq DNA PCR Free library preparation
        • Standard vs Low Input Workflow selection FAQ for Illumina DNA PCR Free prep
        • Using dried blood spots as input into the Illumina DNA PCR Free library prep kit questions
        • What is the Illumina Lysis Reagent Kit, and when should it be used?
        • What loading concentration is recommended for Illumina DNA Prep on MiSeq v2 kits?
        • When to quantify libraries and method of quantification for Illumina DNA PCR Free libraries
        • Which Nextera Index Kits come in plate or tube format?
        • Whole blood input requirements for Illumina DNA PCR Free
      • Reference Material
        • Best practices for storage of bead normalized Nextera XT libraries
        • Considerations before starting the Illumina DNA PCR Free library preparation
        • Considerations for choosing an Illumina DNA PCR Free library preparation workflow
        • Converting ng/µl to nM when calculating dsDNA library concentration
        • Custom primer requirements for the Illumina DNA PCR Free Prep, Tagmentation kit
        • Double sided size selection and bead clean up
        • Illumina DNA PCR Free Tagmentation Introduction Support Webinar Video
        • Illumina DNA Prep Tagmentation Introduction Support Webinar Video
        • Introducing the Illumina Tagment DNA TDE1 Enzyme and Buffer Kits
        • Library quantification and manual normalization of Nextera XT libraries
        • Nextera XT Sample Preparation and Usage Support Webinar Video
        • Nextera XT input considerations
        • Plate layout for Nextera DNA Combinatorial Dual (CD) indexes
        • Product page and support page for TruSeq DNA Nano
        • Product page and support page for TruSeq DNA PCR Free
        • Product page and support page links for Illumina Complete Long Read Prep (ICLR), Human
        • Sequencing information for Illumina DNA Prep (M) Tagmentation libraries
        • What reagents are available for ATAC Seq library preparation?
        • i7 and i5 indexes in each Nextera XT index kit
        • 双链DNA文库浓度的计算:从质量浓度(ng/µl)转化为摩尔浓度(nM)的换算公式
      • Troubleshooting
        • Overtagmentation of Nextera XT libraries
        • Undertagmentation of Nextera XT libraries
    • Enrichment Based Library Prep
      • FAQ
        • Design and coverage of the Respiratory Virus Oligos Panel v2 (RVOP v2)
        • Frequently Asked Questions for Illumina FFPE DNA Prep with Exome 2.5 Enrichment
        • How many samples can be prepared with the Respiratory Pathogen ID/AMR Enrichment Panel (RPIP) Kit?
        • How to pool Illumina DNA Prep with Exome 2.5 libraries on the NextSeq series and NovaSeq 6000?
        • Illumina RNA Prep Tagmentation (L) with Enrichment Input FAQs
        • Illumina RNA Prep Tagmentation (L) with Enrichment Protocol FAQs
        • Illumina RNA Prep Tagmentation (L) with Enrichment Sequencing FAQs
        • Is there a gene list for the Illumina Coding Exome Panel?
        • Product page and support page for the Respiratory Pathogen ID/AMR Enrichment Panel (RPIP) Kit
        • Product page and support page links for Illumina DNA Prep with Exome 2.5 Enrichment
        • Viral Surveillance Panel (VSP) probes and panel design frequently asked questions (FAQs)
        • Viral Surveillance Panel (VSP) reagents and workflow frequently asked questions (FAQs)
        • Viral Surveillance Panel (VSP) sequencing and analysis frequently asked questions (FAQs)
        • What are the volume and number of enrichment reactions for Illumina Exome Panel (CEX)?
        • What read length and coverage depth should be used for the Urinary Pathogen ID/AMR Panel?
      • Reference Material
        • Final library QC and run setup with the Respiratory Virus Oligos Panel v2 (RVOP v2)
        • Illumina DNA Prep with Enrichment Introduction Support Webinar Video
        • Illumina DNA Prep with Enrichment third party probe panel requirements
        • Illumina DNA Prep with Enrichment – Tagmentation: Best Practices and Troubleshooting Video
        • Illumina DNA Prep with Enrichment(原Nextera Flex for Enrichment)第三方panel要求
        • Illumina RNA Prep with Enrichment Introduction Support Webinar Video
        • Library quantification and QC for Illumina TruSight RNA Pan Cancer libraries
        • Product page and support page links for Illumina Cell Free DNA Prep with Enrichment
        • Product page and support page links for Illumina Complete Long Reads with Enrichment (ICLRE), Human
        • TruSight RNA Fusion Panel product page and support page links
        • TruSight RNA Pan Cancer Panel product page and support page links
        • Understanding library pooling for Illumina enrichment kits
    • Illumina Single Cell RNA Prep
      • FAQ
        • Can vortexing damage cells/nuclei when generating emulsions in the Illumina Single Cell workflow?
        • Why is Illumina Single Cell sequencing read depth based on input cells?
      • Reference Material
        • 0.8X bead cleanup to remove adapter dimers from Illumina Single Cell 3’ RNA Prep libraries
        • Assessing cDNA QC in Illumina Single Cell 3’ RNA Prep
        • Assessing library QC in Illumina Single Cell 3’ RNA Prep
        • Best Practices for Nuclei Isolation with alternative nuclei isolation protocols for Illumina Single Cell
        • Best practices for cell preparation for Illumina Single Cell prep
        • Best practices for using Illumina Single Cell Nuclei Isolation Kits for Illumina Single Cell kits
        • Cell input and volume loading recommendations for Illumina Single Cell 3’ RNA Prep
        • Differences between V4.0PLUS PIPseq and Illumina Single Cell (formerly PIPseq V)
        • Extending cDNA storage time in Illumina Single Cell 3' RNA Prep
        • Illumina Single Cell 3' RNA Preparation library sequencing recommendations and guidelines
        • Illumina Single Cell 3’ RNA Prep cDNA QC best practices
        • Illumina Single Cell 3’ RNA Prep library QC best practices
        • Representative cDNA QC traces for Illumina Single Cell 3’ RNA Prep
        • Representative library QC traces for Illumina Single Cell 3’ RNA Prep
        • Sample Enrichment Best Practices for Illumina Single Cell 3' RNA Prep
        • Selecting cycle numbers for High and Low RNA sample types with Illumina Single Cell RNA prep kits
        • Shelf life of Illumina Single Cell 3' RNA Prep kits
        • The importance of pilot studies for Illumina Single Cell RNA Preparation prior to workflow changes
    • RNA Library Prep
      • FAQ
        • Are technical replicates needed for TruSeq Stranded RNA Seq experiments?
        • Are the IDT for Illumina RNA Index Anchors the same in every well of the plate?
        • COVIDSeq timing and logistics
        • Differences with TruSeq Stranded RNA LS and HS protocols and single and dual index adapters
        • Directional sequencing and strandedness for TruSeq Stranded Total RNA and mRNA libraries
        • Frequently asked questions regarding the Ribo Zero Plant and Globin kits
        • General information on Ribo Zero Plus Microbiome
        • Guidance for RNA isolated from PAXgene Blood RNA kit
        • Illumina RNA Prep Tagmentation (L) with Enrichment General FAQs
        • Illumina Stranded Total RNA Prep Ligation with Ribo Zero Plus FAQs
        • Illumina Stranded mRNA Prep Ligation General FAQs
        • Illumina Stranded mRNA Prep Ligation Input FAQs
        • Illumina Stranded mRNA Prep Ligation Sequencing FAQs
        • Input FAQs for the Illumina TruSight RNA Pan Cancer library preparation kit
        • Input recommendations and considerations for COVIDSeq (RUO) library preparation
        • Overview of the IDT for Illumina RNA UD Index Sets, Ligation
        • Product page and support page links for Illumina Stranded Total RNA with Ribo Zero Plus
        • Product page and support page links for TruSeq Stranded Total RNA
        • Protocol FAQs for Illumina Stranded Total RNA Prep Ligation with Ribo Zero Plus
        • Ribo Zero Plus rRNA Depletion Kit FAQs
        • Sequencing FAQs for Illumina Stranded Total RNA Prep Ligation with Ribo Zero Plus
        • Sequencing TruSeq Small RNA libraries
        • Sequencing depth and read length considerations for TruSeq Stranded RNA libraries
        • Shelf life for the COVIDSeq library preparation kit
        • TruSeq RNA v2 product and support page links
        • TruSeq Small RNA Product and Support Pages
        • TruSeq Small RNA alternative T4 RNA Ligase 2, Deletion Mutant supplier
        • TruSeq Small RNA input best practices
        • TruSeq Stranded RNA Kit FAQs: AMPure clean up and library size
        • TruSeq Stranded RNA kit storage and freeze/thaw recommendations
        • TruSeq Stranded RNA library preparation input requirements
        • TruSeq Stranded mRNA Product and Support Pages
        • What are the main differences between TruSeq Stranded and Illumina Stranded kits?
        • What volume of TruSeq RNA UD indexes should be used during TruSeq Stranded RNA library preparation?
        • mRNA purification and rRNA removal information for TruSeq Stranded RNA workflows
      • Reference Material
        • Anchor ligation best practices for Illumina Stranded mRNA and Total RNA Prep Ligation workflows
        • Best Practices for Ribo Zero Depletion using the TruSeq Stranded Total RNA Protocol
        • Best practices for handling magnetic beads used with Illumina RNA library preparation kits
        • Best practices to minimize rRNA contamination in TruSeq Stranded Total RNA libraries
        • Capture and wash best practices for Illumina RNA Prep with Enrichment (L) Tagmentation
        • Considerations for RNA Seq read length and coverage
        • General best practices for Illumina RNA library preparation kits
        • Library preparation options for sequencing the coronavirus SARS CoV 2 (associated with COVID 19)
        • PhiX requirements for sequencing Illumina Stranded RNA libraries
        • Post amplification clean up best practices for Illumina RNA Prep with Enrichment (L) Tagmentation
        • Product page and support page for Illumina Stranded mRNA Prep, Ligation
        • RNA Sequencing Part I Intro to Illumina's RNA Library Preparation Workflows Support Webinar Video
        • RNA Sequencing Part II Best Practices for Illumina's RNA Prep Protocols Support Webinar Video
        • RNA handling best practices for Illumina RNA library preparation kits
        • RNA测序读长及测序深度的选择
        • Ribo Zero Plus depletion best practices
        • Small RNA Seq Part I Best Practices Support Webinar Video
        • Small RNA Seq Part II Best Practices Support Webinar Video
        • Species compatibility table for Ribo Zero Human/Mouse/Rat and Ribo Zero Gold
        • Species compatibility table for Ribo Zero Plant
        • Types of small RNA and the need for a separate small RNA kit
        • Which reads map to each strand in Stranded RNA workflows, and how is strandedness achieved?
        • rRNA Depletion using Illumina Ribo Zero Plus
      • Troubleshooting
        • How to assess rRNA depletion success before sequencing?
        • Impact of RNA degradation on mRNA seq data
    • Trusight Oncology Tumor
      • FAQ
        • Can DNA fusion calling be performed with TruSight Oncology 500 (TSO500) Tissue data?
        • How many freeze/thaw cycles can TruSight Tumor 15 (TST15) library preparation reagents undergo?
        • Product page and support links for TruSight Oncology 500 (Solid)
        • Product page and support links for TruSight Oncology 500 High Throughput (TSO500 HT)
        • Product page and support links for TruSight Oncology 500 ctDNA
        • Product page and support links for TruSight Oncology UMI Reagents
        • Temperature sensitivity and reagent handling FAQ for TruSight Oncology 500 (TSO500) ctDNA
        • TruSight Oncology 500 ctDNA liquid biopsy information
      • Reference Material
        • Best practices for LNB1 handling in bead based normalization for TruSight Oncology 500 workflows
        • Freeze thaw stability of TruSight Oncology 500 (TSO500) ctDNA input and reagents
        • Freeze thaws and kit stability for TruSight Oncology 500 automation library preparation kits
        • General information regarding RNA samples in the TruSight Oncology 500 (TSO500) workflow
        • Instrument compatibility for sequencing TruSight Oncology 500 (TSO500) ctDNA libraries
        • Limit of detection (LOD) for the TruSight Oncology 500 (TSO500) ctDNA assay
        • Sequencing and coverage requirements for TruSight Oncology 500 (TSO500) ctDNA
        • Supported pooling plexities for TruSight Oncology 500 High Throughput (TSO500HT) libraries
        • TruSight Oncology 500 and TruSight Oncology 500 HT Introduction & Overview Support Webinar Video
        • TruSight Tumor 170 product page and support page links
  • Microarray
    • General
      • FAQ
        • Can Infinium reagents be freeze thawed?
        • Can PGx Microarray Analysis be run without interpretation if interpretation has been quoted?
        • Does DRAGEN Array have a Call Rate limit to estimate gender?
        • How long can bisulfite converted DNA be stored in 20°C for use in Infinium Methylation arrays?
        • How to format file path for DRAGEN Array IDAT sample sheet and GTC sample sheet for Windows OS
        • How to select GenTrain version in GenomeStudio 2.0
        • Infinium Assay Modifications for FFPE samples for Genotyping Arrays
        • Is a sample sheet required to run DRAGEN Array?
        • Is it possible to add or remove fields in DRAGEN Array Cloud Genotyping Final Report?
        • What GTC files can be used with DRAGEN Array Local?
        • What is the correct order of addition of Infinium Amplification reagents?
        • What is the expiration timeline for Infinium BeadChips?
        • What is the limit of detection for LOH/AOH for the CytoSNP 850K array?
        • Which fluorometric quantification method to use for quantifying input DNA for the Infinium Assay?
      • Reference Material
        • Annotation database versions for use with BlueFuse Multi v4.4+
        • BlueFuse Multi Introduction to the Cytochip Module Support Webinar Video
        • CNV Analysis in GenomeStudio using cnvPartition Plug in and Illumina Genome Viewer
        • Can GenomeStudio 2.0 genotyping projects be merged?
        • Can Genotype Call (GTC) files be used as input for GenomeStudio?
        • Comparison of GenomeStudio software versions
        • Confirming identities of replicate & matched samples with SNP targeted assays in methylation arrays
        • CytoSNP 850K v1.3 manifest is updated to revisions D1 and D2
        • DMAP files for all Infinium Arrays are available for two (2) years after the date of manufacture
        • DNA Input Guidelines for Infinium MethylationEPIC Arrays
        • DNA strand designations
        • DesignStudio Microarray Assay Designer Input Target File Templates
        • Evaluation of Infinium Staining Controls in the GenomeStudio Controls Dashboard
        • GenomeStudio Advanced Analysis Tools Support Webinar Video
        • GenomeStudio Genotyping Creating Custom Cluster Files for Infinium Arrays Support Webinar Video
        • GenomeStudio Genotyping Evaluating Infinium Assay Controls Support Webinar Video
        • GenomeStudio Genotyping Introduction Support Webinar Video
        • Guidelines for Sample Preparation for Infinium Genotyping Assay DNA input
        • How to Degas the Te flow Video
        • How to Format Sample Sheet for GenomeStudio Genotyping Video
        • How to Format a Sample Sheet for a GenomeStudio Methylation Project Video
        • How to access custom array product files (manifest and product definition files) in MyIllumina
        • How to calculate and import LogRDev into a GenomeStudio Project
        • How to change the date format in the GenomeStudio Final Report in the Processing Date Field
        • How to degas the Te flow
        • How to evaluate the targeted gene amplification (TGA) for Infinium BeadChips with Enhanced PGx Content
        • How to filter for or exclude a list of a large number of SNPs in GenomeStudio
        • How to generate VCF from Illumina BeadArray data
        • How to generate a Final Report and export a Data Table in GenomeStudio 2011.1 Methylation Module
        • How to interpret DNA strand and allele information for Infinium genotyping array data
        • How to properly configure Decode File Client for networks using proxy servers
        • How to share BaseSpace Microarray project data
        • How to use the Auxiliary File for Infinium BeadChips with Enhanced PGx Content
        • How to use the PLINK Report Plug in in GenomeStudio
        • Improve Infinium data with these updated XStain glass back plate care recommendations
        • Infinium Array Best Practices Support Webinar Video
        • Infinium Assay CNV Analysis using GenomeStudio or BlueFuse Multi Software Support Webinar Video
        • Infinium EX Methylation DNA input guidelines and compatible bisulfite conversion kits
        • Infinium Genotyping Intro to GenomeStudio 2.0 Polyploid Genotyping Module Support Webinar Video
        • Infinium Genotyping Introduction to Beeline 2.0 and Data Analysis Workflows Support Webinar Video
        • Infinium Genotyping Introduction to DesignStudio Microarray Assay Designer Support Webinar Video
        • Infinium Genotyping Strandology Strand designations in Infinium Manifests Support Webinar Video
        • Infinium HD Methylation assay workflow for fresh/frozen versus FFPE samples
        • Infinium Methylation Intro to GenomeStudio and BeadArray Controls Reporter Support Webinar Video
        • Infinium Methylation450K manifest column headings
        • Infinium MethylationEPIC Manifest Column Headings
        • Infinium MethylationEPIC v2.0 Manifest Column Headings
        • Infinium Mouse Methylation BeadChip GenomeStudio controls interpretation
        • Infinium Mouse Methylation Beadchip manifest column headings
        • Infinium genotyping manifest column headings
        • Infinium workflow how to prepare bleach solution (sodium hypochlorite)
        • Infinium基因分型芯片manifest文件中Chr或MapInfo列的值为0时代表什么意思?
        • Manifestology Deciphering Illumina BeadArray Manifests Support Webinar Video
        • Preparation, storage, and use of 95% formamide + EDTA in Infinium assays
        • Proper care of Tecan/IAPS with IAC robot fluidics
        • Recommendations for Infinium Array XC4 reagent use and storage
        • Sample sheet guidelines for GenomeStudio
        • Simple guidelines for identifying top/bottom (TOP/BOT) strand and A/B allele
        • Tecan/IAPS with IAC Power Cycle Instructions
        • The correct storage temperature for Infinium BeadChips is 2 8°C
        • Tips and tricks for downloading DMAPs using the Decode File Client
        • Types of reports generated in GenomeStudio
        • What does Chr or MapInfo = 0 mean in Infinium genotyping array manifest files?
        • Where to find required license keys / serial numbers for installation of GenomeStudio 2011.1
        • Which bisulfite conversion kit should be used for the Infinium Methylation arrays?
        • Which files are required and optional when creating a GenomeStudio project?
        • Why there may be multiple assays targeting the same SNP marker in array manifests
        • XStain glass back plate care recommendations for Infinium EX Assays
        • 所有Infinium 芯片的DMAP文件自生产之日起两年内可下载
        • 确认TOP/BOT链和A/B等位基因的基本规则
      • Troubleshooting
        • A quick visualization method to help determine the sex of given samples in a GenomeStudio Methylation project
        • Create a batch file for simple BeadChip accessioning to Illumina Microarray Analytics PGx Analysis
        • Decode File Client Troubleshooting Guide
        • How to check Infinium samples for possible cross sample contamination in GenomeStudio
        • How to obtain Unmethylated and Methylated signal in GenomeStudio for each CpG in MethylationEPIC Array
        • Hybridization and X stain Infinium Assay Troubleshooting Guide
        • Important information about using 12 digit BeadChip barcodes in GenomeStudio sample sheets
        • Precipitation and Resuspension Troubleshooting Guide in Infinium Assay
        • Single sample group formation to reduce GenomeStudio Methylation Module crashing during analysis
        • Troubleshooting "Error in reading file Normalization Transformation incorrect version" in Array ACLI
        • Troubleshooting "Unknown cluster statistics version" error in GenomeStudio 2011.1
        • Troubleshooting the Infinium HD FFPE QC Assay Protocol
        • What are the ch probes in the MethylationEPIC Array manifest?
        • What is the correct format for DRAGEN Array IDAT CSV Sample Sheet?
    • Ancillary Array Equipment
      • FAQ
        • What are the dimensions of the Infinium hybridization oven?
      • Reference Material
        • Updated guidance for using new blue Infinium Staining Set
    • Hamilton
      • Reference Material
        • Infinium Amplification System (IAS Hamilton) power cycle instructions
    • IAPS
      • Reference Material
        • Proper care of IAPS with ILASS robot fluidics
        • Tecan/IAPS with ILASS Power Cycle Instructions
    • LIMS
      • Reference Material
        • Investigation of Log4j Vulnerability with Illumina LIMS
        • The required version of Java for Illumina LIMS is Java 8 for the foreseeable future
    • NextSeq 500/550
      • FAQ
        • Which Infinium BeadChip types can be scanned using the NextSeq 550?
      • Reference Material
        • Scan features differences between the iScan and NextSeq 550
    • Tecan
      • FAQ
        • Cleaning agents specifications and applications for Tecan (LiHa and RoMa) parts
      • Reference Material
        • Investigation of Log4j Vulnerability with Tecan and Hamilton Liquid Handlers
    • iScan
      • FAQ
        • Can a single Autoloader/iScan run contain multiple types of beadchips?
        • Can an iScan send notifications when it has completed scanning Infinium beadchips?
        • How to determine the iScan Control Software version
        • What is the required iScan Control Software version and scan setting for Infinium EX BeadChips?
      • Reference Material
        • How to set up Autoconvert in the iScan Control Software
        • Infinium Protocol Video
        • Port Reset and Power Cycle Sequence for iScan or HiScan, without an AutoLoader
        • Power Cycle and Port Reset Sequence for one iScan with one AutoLoader
        • Power Cycle and Port Reset Sequence for two iScans and one AutoLoader
        • Using network file shares for data input and output in iScan Control Software (ICS)
      • Troubleshooting
        • How to check for corrupt DMAPs before scanning BeadChips using the iScan
  • Software
    • General
      • FAQ
        • Does TruSight Oncology 500 (TSO500) use a segmentation algorithm to determine the genomic coordinates of a copy number call?
        • How is zygosity assigned for mitochondrial variant calls with DRAGEN?
        • How to invite users to a Project in Illumina Connected Analytics v2
        • How to run the DRAGEN MSI Pipeline
        • How to use the 'OverrideCycles' setting in the Run Planning User Interface
        • Is there a certain number of times that users can requeue local analysis?
        • Which orientation are the indexes in Top_Unknown_Barcodes output from BclConvert?
        • Why are underscores in sample names changed to dashes in the FASTQ file names?
      • Reference Material
        • Adapter trimming Why are adapter sequences trimmed from only the 3' ends of reads
        • BaseSpace FASTQ Processing Tools for Data Analysis Support Webinar Video
        • Creating a custom genome for NovaSeq X Series
        • FASTQ files explained
        • FASTQ文件解读
        • Generating checksums for a file
        • Guidelines for reverse complementing i5 sequences for demultiplexing
        • How to avoid Ns in small RNA or short reads when running BCL Convert
        • How to convert a custom BED file to a manifest file for enrichment analysis
        • How to realign a CRAM file to a new reference with DRAGEN
        • How to set OverrideCycles values for variable index sequence lengths on the NovaSeq X/X Plus
        • How to unlock BioSamples in BaseSpace using BaseSpace Command Line Interface (CLI)
        • How to use Exome 2.5 Panel of Normals reference for germline deletion/duplication analysis
        • Illumina DRAGEN Bio IT Platform What is new with version 3.10 updates Support Webinar Video
        • Illumina DRAGEN Bio IT Platform What is new with version 3.9 updates Support Webinar Video
        • Illumina DRAGEN Bio IT Platform Ultra Rapid Analysis of Next Generation Data Support Webinar Video
        • Illumina DRAGEN Bio IT Platform What is new with version 3.5 updates Support Webinar Video
        • Illumina DRAGEN Bio IT Platform What is new with version 3.6 updates Support Webinar Video
        • Illumina DRAGEN Bio IT Platform What is new with version 3.7 Support Webinar Video
        • Illumina DRAGEN Bio IT Platform What is new with version 3.8 updates Support Webinar Video
        • Illumina DRAGEN v4.0 Unlock the Full Potential of Genomics Support Webinar Video
        • Information on leading r for UMIs when demultiplexing NextSeq 1000/2000 or NovaSeq X series data with BCL Convert
        • Instructions on how to restart run upload to BaseSpace using BaseSpace Broker
        • Investigation of Log4j Vulnerability status for Illumina off instrument software products
        • Investigation of Log4j Vulnerability with the Illumina DRAGEN Bio IT Platform
        • Investigation of Log4j Vulnerability
        • Maximum number of cycles for index reads on the NovaSeq X Series
        • Metagenomic Sequencing Introduction to Data Analysis Support Webinar Video
        • NextSeq 1000/2000 Introduction to Analysis with DRAGEN Support Webinar Video
        • Sequencing Data Analysis Introduction to Key Concepts Support Webinar Video
        • Trimming T overhang options for Illumina Stranded mRNA and Illumina Stranded Total RNA workflows
        • TruSight Oncology 500 Assays and Analysis Options
        • Upgrading from bcl2fastq to BCL Convert
        • Using an N wildcard in index sequences in different Illumina FastQ generation software
        • Why is allowing mismatches when demultiplexing desirable?
      • Troubleshooting
        • Does the DRAGEN pipeline support VQSR (Variant Quality Score Recalibration)?
        • How to troubleshoot a "None of the specified endpoints were reachable" error in Local Run Manager
        • Illumina Connected Analytics (ICA) kernel cannot connect in a Bench Workspaces
        • Options for demultiplexing libraries with different index lengths in the same sequencing run
        • Sample specific nucleotide error bias estimation filter and systematic noise filter in DRAGEN
        • Troubleshooting LRM analysis module not available at run start on NextSeq 500/550
    • 3rd Party Software
      • FAQ
        • Information Regarding OpenSSL v3 Vulnerability
      • Reference Material
        • How to upload files to Illumina Kiteworks using command line tools
    • Cloud Software
      • FAQ
        • Are the files for SAV available from BaseSpace if the run was performed with only Run Monitoring?
        • BaseSpace Sequence Hub archival storage FAQs
        • BaseSpace v7.12 update frequently asked questions
        • Can BaseSpace files be transferred directly to AWS (Amazon Web Services)?
        • Coverage and frequency requirements for variants in FASTA output from the DRAGEN COVID Lineage app
        • Different coverage reports from the DRAGEN Enrichment analysis
        • How does BaseSpace Command Line Interface (CLI) check the integrity of file uploads?
        • How to archive and retrieve data in BaseSpace using BaseSpace Command Line Interface (CLI) commands?
        • How to generate Audit Logs in BaseSpace Sequence Hub?
        • How to requeue FASTQ Generation using BCL Convert on BaseSpace for the NextSeq 1000/2000
        • How to run the DRAGEN PopGen app on BaseSpace?
        • How to transfer runs/datasets between different regional servers using BaseSpace CLI
        • How to use a custom virus primer BED file with the DRAGEN COVID Lineage app on BaseSpace
        • How to use an API call to download FastQ files from BaseSpace
        • How to view files from Illumina Connected Analytics in Integrative Genomics Viewer
        • Running DRAGEN Amplicon 3.10.4 on BaseSpace on a single sample type with a hg38 reference
        • What are the different BaseSpace Account Types?
        • What is the BaseSpace data deletion policy?
        • What is the process for renewing a BaseSpace Professional Workgroup account?
      • Reference Material
        • BaseSpace 16S Metagenomics App General Information
        • BaseSpace Command Line Tools for Basic Analysis Part I Support Webinar Video
        • BaseSpace Command Line Tools for Basic Analysis Part II Support Webinar Video
        • BaseSpace Data Model Explained
        • BaseSpace Informatics Suite Intro to Cohort Analyzer and Correlation Engine Support Webinar Video
        • BaseSpace Sequence Hub Introduction and Analysis Overview Support Webinar Video
        • BaseSpace Sequence Hub Prep Tab How to create a custom library prep kit
        • BaseSpace Sequence Hub informational resources
        • BaseSpace Sequence Hub server instances explained
        • DRAGEN Enrichment App in BaseSpace
        • DRAGEN Metagenomics Pipeline in BaseSpace general information
        • Enrichment Data Analysis Introduction Support Webinar Video
        • How to Analyze Data using BaseSpace DRAGEN COVID Lineage App Video
        • How to Requeue Analysis for a NextSeq 1000/2000 run using BaseSpace BCL Convert Video
        • How to Requeue a Run using BaseSpace Sequence Hub Video
        • How to accept BaseSpace transfer ownership requests in a workgroup context
        • How to authenticate BaseSpace CLI to another region or Enterprise domain
        • How to authenticate BaseSpace CLI to workgroup
        • How to change ownership of a BaseSpace Professional Workgroup or Enterprise Domain
        • How to concatenate the FASTQ files from different lanes
        • How to configure Single Sign On (SSO) for an Illumina Enterprise domain using Azure
        • How to configure Single Sign On (SSO) for an Illumina Enterprise domain using Okta
        • How to download FASTQs from specific lanes using Basespace CLI
        • How to download only FASTQs from a BaseSpace project using BaseSpace CLI
        • How to download specific file types when downloading runs from BaseSpace using BaseSpace CLI
        • How to edit a sample sheet and requeue an analysis in BaseSpace Sequence Hub
        • How to edit sample indexes in BaseSpace Sequence Hub PrepTab
        • How to find the Demultiplexing Summary and SampleSheetUsed in BaseSpace?
        • How to generate TruSight Software Suite (TSS) API keys
        • How to get started with BaseSpace Command Line Interface (CLI) Video
        • How to import files into a BaseSpace Project using the web Interface
        • How to install the BaseSpace Sequence Hub Downloader on Mac OS
        • How to invite users to a workgroup on BaseSpace Sequence Hub (Professional or Enterprise tier)
        • How to launch any app in BaseSpace using BaseSpace Command Line Interface (CLI)
        • How to launch the DRAGEN COVID Lineage app using BaseSpace CLI?
        • How to manage status.illumina.com subscriptions
        • How to requeue (re launch) the autolaunch analysis on BaseSpace
        • How to requeue analysis of a NextSeq 1000/2000 run in BaseSpace Sequence Hub
        • How to set the default location for uploaded runs to BaseSpace
        • How to share BaseSpace Sequence Hub sequencing data from enterprise domain to public domain
        • How to share BaseSpace sequencing runs and project data using 'Get Link' option
        • How to transfer data in BaseSpace from Public Domain to Enterprise Domain
        • How to unlock a Biosample in BaseSpace that is locked due to Biosample Data Aggregation
        • How to upload a run to BaseSpace using BaseSpace Command Line Interface (CLI)
        • How to upload and download FASTQ files to BaseSpace with BaseSpace CLI
        • How to use filters with Variant Interpreter
        • How to use the DRAGEN TSO500 v1.1/DRAGEN TSO500 ctDNA v1.2 pipelines on ICA using FASTQ files
        • How to use the Resources page to add custom reference files for Run Planning for NovaSeq X/X Plus runs
        • How to use the TruSight Software Suite (TSS) CLI uploader
        • Instructions for using BaseSpace CLI on Windows to upload runs
        • Investigation of Log4j Vulnerability with BaseSpace Sequence Hub (BSSH) Cloud
        • Investigation of Log4j Vulnerability with Illumina Connected Analytics (ICA)
        • Investigation of Log4j Vulnerability with TruSight Software Suite (TSS)
        • Panel of Normals TruSight Hereditary Cancer Panel datasets for copy number variations in BaseSpace
        • RNA Sequencing Part III Introduction to Analysis Support Webinar Video
        • Reference genome and target regions BED file for the DRAGEN PopGen app
        • Transferring data between a personal account and a Professional workgroup in BaseSpace Sequence Hub
        • Troubleshooting demultiplexing issues using BaseSpace Sequence Hub and bcl2fastq2 v2.17+
        • TruSight Oncology 500 version 2 Analysis Overview Support Webinar Video
        • Uploading Runs BaseSpace Command Line Interface (CLI) Video
        • Uploading and downloading FASTQ files with BaseSpace CLI Video
        • What is restricted when a BaseSpace Basic user exceeds 1TB storage limit
        • Where data copied from BaseSpace to ICA gets stored on ICA
        • Which BaseSpace apps and features use Illumina Connected Analytics
        • 使用BaseSpace基因云计算平台和bcl2fastq2 2.17以及以上版本软件时,如何排查拆分异常情况
      • Troubleshooting
        • BaseSpace Command Line Interface upload failure with request signature error
        • BaseSpace and Proactive outage resource page
        • Creating custom reference genomes (non human) to use with DRAGEN apps on BaseSpace Sequence Hub
        • DRAGEN Imputation App v 4.0.3 on BaseSpace
        • Details about DRAGEN Metagenomics Kraken2 Database
        • Emedgene troubleshooting information request form
        • How to demultiplex mixed libraries on the same lane in BaseSpace
        • How to download files needed for Sequencing Analysis Viewer from BaseSpace using BaseSpace CLI
        • How to recover a BaseSpace password reset email
        • How to restart Universal Copy Service when a run is stuck uploading to BaseSpace Sequence Hub
        • How to review bsshoutput.json to assess BaseSpace file project mapping from Instrument Autolaunch Analysis
        • Requirements for uploading FASTQ Files to BaseSpace via web interface
        • Troubleshooting "Failed to validate certificate" error when launching IGV BaseSpace Application
        • Troubleshooting DRAGEN analysis error UMI processing is enabled but QNAME does not have UMI section
        • Troubleshooting Illumina Service Connector not connected for Window computer
        • Troubleshooting emptying the trash in BaseSpace Sequence Hub
        • Understanding how instrument autolaunch analysis maps to BaseSpace projects
        • What to do if a BaseSpace analysis is stuck or stalled running
        • Why an appsession API call may only returning a subset of datasets from BaseSpace
    • On Instrument Analysis Software
      • FAQ
        • Can a run be started on the NovaSeq X/X Plus while an analysis is ongoing?
        • Can the MiSeq System generate FASTQ files?
        • MiSeq Reporter Common Questions
      • Reference Material
        • Allowed characters for Run Names and Sample IDs in Local Run Manager v3
        • Comparison of MiSeq Reporter and Local Run Manager analysis workflows
        • Connecting remotely to Local Run Manager v4 on instrument
        • How to Specify Adapters for Trimming in Local Run Manager
        • How to add a Custom Reference Genome in Local Run Manager
        • How to generate FASTQ files for index reads in MiSeq Reporter
        • How to generate FASTQ files from an incomplete run in MiSeq Reporter
        • How to requeue analysis of a run with MiSeq Reporter
        • How to set up an AmpliSeq for Illumina run using Local Run Manager v1.3 for MiniSeq
        • How to use a custom library prep kit in Local Run Manager v2
        • How to use custom library prep and index kits with Local Run Manager v3 and v4
        • Local Run Manager How to create FASTQ files for index reads
        • Local Run Manager v2 (Research Use Only) Introduction On and Off Instrument Support Webinar Video
        • Local Run Manager v3 with MiSeq Control Software v4 Introduction Support Webinar Video
        • Local Run Manager: 如何利用不完整的测序结果生成FASTQ文件
        • Local Run Manager: 如何对已经完成的测序进行重新分析(Requeue)
        • Troubleshooting demultiplexing issues using Local Run Manager
        • Troubleshooting demultiplexing issues using MiSeq Reporter
        • Troubleshooting demultiplexing issues using onboard DRAGEN on the NextSeq 1000/2000
        • Using Illumina Experiment Manager (IEM) to Create a Sample Sheet for Illumina Libraries Video
        • 如何使MiSeq Reporter生成Index Reads 的FASTQ文件
        • 如何使用MiSeq Reporter从未完成的run中创建出FASTQ文件
        • 如何使用MiSeq Reporter重新分析一个run?
        • 如何将桌面测序仪和Local Run Manager与网络存储位置进行连接
      • Troubleshooting
        • Creating a custom DRAGEN reference genome for NextSeq1000/2000 analysis
        • Generate FASTQ from an incomplete run in Local Run Manager
        • How to remove a BCL file from analysis in MiSeq Reporter or Local Run Manager
        • Unlocking an account or resetting the password on Local Run Manager version 4
    • On Premises Software
      • FAQ
        • How to avoid Ns in small RNA reads or short reads after running bcl2fastq2?
        • How to calculate the DRAGEN license quota
        • How to cold boot or power cycle a DRAGEN server?
        • How to find the FPGA serial number on a DRAGEN server?
        • How to generate FASTQs for a run without specifying indexes using BCL Convert
        • How to output both gVCF and VCF in a single DRAGEN run?
        • How to use a custom cross contamination VCF file with DRAGEN
        • Visualizing the DRAGEN Evidence BAM in Integrative Genomics Viewer (IGV)
      • Reference Material
        • BCL Convert and index UMIs How to demultiplex samples and output a UMI FASTQ
        • Bcl2Fastq v2+Introduction Support Webinar Video
        • Considerations for using a DRAGEN Server with an automated job scheduler
        • DRAGEN Bio IT Platform v3 Oracle 8 upgrade instructions
        • DRAGEN Bio IT Platform v4 Oracle 8 upgrade instructions
        • DRAGEN TSO500 Analysis on Illumina Connected Analytics Video
        • DRAGEN TruSight Oncology 500 (TSO500) co install options
        • How to add a custom library prep kit to Illumina Experiment Manager
        • How to create a Project connector in Illumina Connected Analytics Video
        • How to demultiplex a run without using either the i7 index or the i5 index using bcl2fastq2
        • How to demultiplex a sequencing run without using either the i7 or the i5 index using BCL Convert
        • How to demultiplex multiple library types on the same run with multiple lanes using bcl2fastq2
        • How to install an Illumina Connected Analytics service connector on Linux Video
        • How to install an Illumina Connected Analytics service connector on Windows Video
        • How to install an Illumina Connected Analytics service connector on macOS Video
        • How to parse the Counts sparse matrix file output by the DRAGEN scRNA and scATAC pipelines
        • How to set up analysis for AmpliSeq for Illumina libraries with off instrument Local Run Manager 2
        • How to update the DRAGEN BIOS version to reduce v4 server fan noise
        • Information on BCL Convert software releases
        • Investigation of Log4j Vulnerability with Emedgene
        • Labeled components of a v1 sample sheet
        • Recommendation to abort ongoing DRAGEN analysis
        • Running DRAGEN analyses with ORA files as input
      • Troubleshooting
        • Cannot specify ' with failed reads' with cbcl input data." error in bcl2fastq2
        • Considerations when using DRAGEN TruSight Oncology 500 Solid v2.1 Sample Sheet
        • DRAGEN TSO500 ctDNA v2.1.1 install fails or breaks existing DRAGEN TSO500 installation
        • DRAGEN installation failed due to lack of disk space in root volume
        • DRAGEN license information and installation
        • Download links for DRAGEN Reference genome FASTA files
        • How to demultiplex an incomplete run using bcl2fastq2 and BCL Convert
        • How to troubleshoot barcode collision error when running bcl2fastq2
        • Instructions to back up DRAGEN license files when upgrading the operating system on a DRAGEN server
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  1. Instrumentation
  2. Instrument Administration
  3. Troubleshooting

Troubleshooting Windows services menu shows Log On As options greyed out

Last updated 1 year ago

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The services application must be running as an administrator to modify the Log On as account. If the services application is opened and run from a standard account, the Log On As options will be read only, and non-modifiable.

Example:

Resolve by following one of the below options:

  1. Switch from the standard account to an administrator account and update.

  2. Close the Services application and Re-Open, being sure to right-click and run as administrator to make changes. Enter the administrator password when prompted.

For any feedback or questions regarding this article (Illumina Knowledge Article #7862), contact Illumina Technical Support .

techsupport@illumina.com