# Supported pooling plexities for TruSight Oncology 500 High Throughput (TSO500HT) libraries
The following are the maximum numbers of TruSight Oncology 500 High-Throughput (TSO500HT) samples that can be multiplexed for sequencing on supported sequencing systems.
NovaSeq 6000
NextSeq 500/550
NovaSeq X Series
The pooling recommendations are also given on the [TruSight Oncology 500 High Throughput Compatible Products](https://support.illumina.com/sequencing/sequencing_kits/trusight-oncology-500-high-throughput-support/trusight-oncology-500-high-throughput-compatible-products.html) page and the [TruSight Oncology 500 High-Throughput on the NovaSeq X Series](https://www.illumina.com/content/dam/illumina/gcs/assembled-assets/marketing-literature/tso500-ht-novaseq-x-tech-note-m-gl-02869/tso500-ht-novaseq-x-tech-note-m-gl-02869.pdf) technical note.
The same plexities are recommended for DNA-only sequencing as well; other combinations have not been tested.
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