# Supported pooling plexities for TruSight Oncology 500 High Throughput (TSO500HT) libraries

The following are the maximum numbers of TruSight Oncology 500 High-Throughput (TSO500HT) samples that can be multiplexed for sequencing on supported sequencing systems.

NovaSeq 6000

![](https://761066130-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FGM9W2DuBTgEXv1ClCm8H%2Fuploads%2Fgit-blob-88cd0649e0a1feebd61a317da8d91d5de38e5685%2Fimage1.jpg?alt=media)

NextSeq 500/550

![](https://761066130-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FGM9W2DuBTgEXv1ClCm8H%2Fuploads%2Fgit-blob-67d7a2978d771e701f4ef1c848f672c998dce572%2Fimage2.jpg?alt=media)

NovaSeq X Series

![](https://761066130-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FGM9W2DuBTgEXv1ClCm8H%2Fuploads%2Fgit-blob-b29dbd40186e841c72a9c7cfaab110d6932d95c2%2Fimage3.jpg?alt=media)

The pooling recommendations are also given on the [TruSight Oncology 500 High Throughput Compatible Products](https://support.illumina.com/sequencing/sequencing_kits/trusight-oncology-500-high-throughput-support/trusight-oncology-500-high-throughput-compatible-products.html) page and the [TruSight Oncology 500 High-Throughput on the NovaSeq X Series](https://www.illumina.com/content/dam/illumina/gcs/assembled-assets/marketing-literature/tso500-ht-novaseq-x-tech-note-m-gl-02869/tso500-ht-novaseq-x-tech-note-m-gl-02869.pdf) technical note.

The same plexities are recommended for DNA-only sequencing as well; other combinations have not been tested.

\
\
\ <br>

|                                                                                                                                                                                                                                                                                                                                                                              |
| :--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------: |
| *For any feedback or questions regarding this article (Illumina Knowledge Article #2809), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000002809%20-%20Library%20Preparation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |