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How to resolve Network Connection warnings during Pre Run Checks on the NovaSeq X/X Plus
Background
During Pre-Run Checks, the instrument will perform a series of checks to verify that the instrument is ready to perform a sequencing run. One of the first checks that is performed is verifying that the networked External Storage location is accessible, and the instrument has the appropriate read/write permissions.
If there are issues with this connectivity check, the following warning will present to the user.
Error: The run data will not be copied to the network, and will need to be copied to the network folder manually.
If this warning presents during Pre-Run Checks, see the following troubleshooting steps.
Troubleshooting steps
- 1.The Warning message will present the option for the user to select Continue and proceed with the Pre-Run Checks.
- Important: If this option is selected, the Control Software will not automatically transfer the run data to External Storage and the data will need to be transferred manually.
- See Knowledge Base Article How to access network storage locations in Linux on the NovaSeq X/X Plus for instructions on how to mount a network share for manual data transfers.
- 2.Alternatively, the user can select Return to Start and then reset the External Storage share, then retry the Pre-Run Checks.
Resetting the External Storage
- 1.Before starting, make sure that the following information is available from the local IT or network data storage management team:
- 1.The fully qualified Universal Naming Convention (UNC) path to the network share.
- 2.The name of the desired output folder on the network share.
- 3.Network username and password (SMB Shares only).
- 2.Within the Control Software, select the instrument icon, then Settings, then External Storage.
- 3.Under Server Location table, locate the network share that the instrument was unable to connect with during Pre-Run Checks and select Remove volume.
- 4.After removing the network share, select Add network storage and then select SMB or NFS radio button depending on the protocol used:
- 1.For SMB Shares:
- 1.Input the fully qualified UNC path to the share in the Server Location field.
- 2.Input the Domain (optional).
- 3.Input the network Username and Password.
- 4.Select whether or not to require Encryption during transfer.
- 2.For NFS Shares:
- 1.Input the fully qualified UNC path to the share in the Server Location field.
- 2.Input the Domain (optional).
- 3.Input admin for both the Username and Password.
- 5.Once the required information is entered, select Test Configuration.
- 6.If the test is successful, select Save.
Setting the Output Folder
- 1.After configuring the Network Storage, add the Output Folder by selecting + Add Folders then Add Folder.
- The intended output folder should be created on the network share since the Control Software does not permit the creation of folders.
- 2.Select the Server location from the dropdown menu, and then select the Output folder from the populated Available Folders list.
- 3.Optional: Input a nickname for the Output folder and select Save, then select Save again on the Output Folders page.
- 4.Select the X at the bottom of the screen to return to the Settings menu, then select the X at the bottom of the screen to return to the Control Software Home screen.
After the External Location has been reset and the Output folder selected, proceed with repeating the Run Setup and Pre-Run Checks.
If the network connectivity pre-run check continues to present the warning above, contact Illumina Technical Support.
For any feedback or questions regarding this article (Illumina Knowledge Article #8308), contact Illumina Technical Support [email protected]. |