Troubleshooting "Initialize Cameras before capturing images" error on the MiSeq

Users may encounter an "Initialize Cameras before capturing images" error message during the first cycles of a MiSeq runs. This error may occur if the previous run on the instrument ended early due to a communications error that caused the cameras to shut down.

An example of this type of error is an FPGA error, which can be investigated by searching for Knowledge Base article Troubleshooting FPGA Timeout Errors on the MiSeq. If the user did not power cycle after such an error, this can cause an "Initialize Cameras before capturing images" error to occur.

Troubleshooting steps:

• Power cycle the instrument in order to re-initialize the cameras, power cycle the instrument.

  • For further information, search for Knowledge Base articles How to shut down or power cycle the MiSeq and How To Power Cycle the MiSeq Video.

• Check D:\ drive space; if full delete older data (after backing up to an off-instrument location).

  • For further information, search for Knowledge Base articles Run folder sizes and Disk Space on the MiSeq and Troubleshooting Free Disk Space errors during Pre-run Checks on MiSeq.

If the instrument fails to initialize or the error persists, contact Illumina Technical Support.

For any feedback or questions regarding this article (Illumina Knowledge Article #7442), contact Illumina Technical Support [e-mail].