> For the complete documentation index, see [llms.txt](https://knowledge.illumina.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://knowledge.illumina.com/instrumentation/miseq/instrumentation-miseq-troubleshooting-list/000007442.md).

# Troubleshooting "Initialize Cameras before capturing images" error on the MiSeq

Users may encounter an "Initialize Cameras before capturing images" error message during the first cycle of a MiSeq run. This error may occur if the previous run on the instrument ended early due to a communications error that caused the cameras to shut down.

![](https://761066130-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FGM9W2DuBTgEXv1ClCm8H%2Fuploads%2Fgit-blob-e684b6773c79b7c7e85c7b0d6db6473f59bdbdfe%2Fimage1.jpg?alt=media\&token=973c5cf5-af8d-4d05-9c8e-5de91d56ee14)

An example of this type of error is an FPGA error; see more in the Knowledge Base article[Troubleshooting FPGA Timeout Errors on the MiSeq](/instrumentation/miseq-ruo/instrumentation-miseq-ruo-troubleshooting-list/000001444.md). If the user did not power cycle after such an error, this can cause an "Initialize Cameras before capturing images" error to occur.

**Note**: This error may cause a cycle 1 failure and mark consumables as "used". If this is the case, please contact [Illumina Technical Support](https://www.illumina.com/company/contact-us.html#/united-states/technical-support) for assistance.

**Troubleshooting steps:**

* **Power cycle** the instrument in order to re-initialize the cameras.
  * For further information, see the Knowledge Base articles [How to Power Cycle the MiSeq](/instrumentation/miseq-ruo/instrumentation-miseq-ruo-reference_material-list/000001894.md) and [How To Power Cycle the MiSeq Video](/instrumentation/miseq-ruo/instrumentation-miseq-ruo-reference_material-list/000005871.md).
* Check D:\ **drive space**. If an earlier run prematurely terminated, there may be old files taking up space in the old **MiSeqTemp** and **MiSeqAnalysis** run folder locations:
  * D:\Illumina\MiSeqTemp\&lt;old run folder> and D:\Illumina\MiSeqAnalysis\&lt;old run folder>
  * Delete older data from any prematurely terminated runs.
  * Additionally, detele any older run folders present from completed runs. Ensure they are backed up off-instrument before being deleted.
  * For further information, review the Knowledge Base articles [Run folder sizes and Disk Space on the MiSeq](/instrumentation/miseq-ruo/instrumentation-miseq-ruo-reference_material-list/000006711.md) and [Troubleshooting Free Disk Space errors during Pre-run Checks on MiSeq](/instrumentation/miseq-ruo/instrumentation-miseq-ruo-troubleshooting-list/000003753.md)

If the instrument fails to initialize or the error persists, contact [Illumina Technical Support](https://www.illumina.com/company/contact-us.html#/united-states/technical-support).

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| *For any feedback or questions regarding this article (Illumina Knowledge Article #7442), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000007442%20-%20Instrumentation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


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