# Representative cDNA QC traces for Illumina Single Cell 3’ RNA Prep
**cDNA peaks, distribution, and average fragment size can vary based on sample type and experimental condition(s).**
The following TapeStation-generated traces serve as representative examples of satisfactory cDNA QC profiles generated during the *Check QC Product* step in the [Illumina Single Cell 3’ RNA Prep workflow](https://support.illumina.com/sequencing/sequencing_kits/illumina-single-cell-prep/documentation.html).
**Human peripheral blood mononuclear cells (PBMCs)**
**Human HEK 293T & mouse NIH 3T3 cell line mixture**
**Mouse frozen tissue: unfixed brain nuclei**
**Mouse frozen tissue: DSP-methanol fixed brain nuclei**
**Mouse frozen tissue: unfixed mouse diaphragm**
**Mouse frozen tissue: unfixed mouse heart**
**Mouse frozen tissue: unfixed lung nuclei**
**Mouse frozen tissue: DSP-methanol fixed lung nuclei (1-month storage)**
For example final library traces produced using similar sample types, see [Representative library QC traces for Illumina Single Cell 3’ RNA Prep](/library-preparation/illumina-single-cell-rna-prep/library-preparation-illumina-single-cell-rna-prep-reference_material-list/000009528.md)
For more information related to cDNA QC product assessment refer to:
* [Illumina Single Cell 3’ RNA Prep cDNA QC best practices](/library-preparation/illumina-single-cell-rna-prep/library-preparation-illumina-single-cell-rna-prep-reference_material-list/000009523.md)
* [Assessing cDNA QC in Illumina Single Cell 3’ RNA Prep](/library-preparation/illumina-single-cell-rna-prep/library-preparation-illumina-single-cell-rna-prep-reference_material-list/000009524.md)
* [Illumina Single Cell 3' RNA Prep Training Packet](https://support.illumina.com/sequencing/sequencing_kits/illumina-single-cell-prep.html)
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| *For any feedback or questions regarding this article (Illumina Knowledge Article #9525), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000009524%20-%20Library%20Preparation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |
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