Troubleshooting running low diversity samples with low PhiX spike in on the iSeq 100
The iSeq 100 uses one-channel chemistry. To obtain high-quality data on an iSeq 100 for low-diversity libraries, a spike in at least 5% PhiX. This will increase the amount of signal in channels with lower signal in each cycle and help to balance the base diversity, resulting in high-quality base calls.
For further information, search for Knowledge Base article How much PhiX spike-in is recommended when sequencing low diversity libraries on Illumina platforms?.
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