Troubleshooting running low diversity samples with low PhiX spike-in on the iSeq 100

The iSeq 100 uses one-channel chemistry. To obtain high-quality data on an iSeq 100 for low-diversity libraries, spike in at least 5% PhiX. This increases the amount of signal in channels with lower signal during each cycle and helps balance the base diversity, resulting in high-quality base calls.

For further information, review the Knowledge Base article How much PhiX spike-in is recommended when sequencing low diversity libraries on Illumina platforms?

For any feedback or questions regarding this article (Illumina Knowledge Article #1369), contact Illumina Technical Support [e-mail].