Troubleshooting Pressure decay or Impedance test stopped with NaN results on the NovaSeq 6000

If Pressure Decay or Impedance test on the NovaSeq 6000 system check stops with NaN result:

• Check pressure sensor readings:

• Close NovaSeq Control Software.

• Open NovaSeq Service Software (NVSS).

• Navigate to Integrated Chassis Assembly.

• Navigate to the Sensors Tab and select the refresh icon for Fluidics.

• If any sensor is >2.5psi, take a screenshot or picture of the result and collect NovaSeq Service Software Logs located under C:\ProgramFile\Illumina\NovaSeq\NovaSeq Service Software\Logs and contact Illumina Technical Support for the next steps.

• Note: Pressure sensors are not used during a run and, therefore, failing sensors will not impact a run performance. The instrument can continue sequencing until an Illumina Field Service Engineer (FSE) can replace the sensors.

For any feedback or questions regarding this article (Illumina Knowledge Article #2492), contact Illumina Technical Support [e-mail].