# Specifications for the iSeq 100 Quality and yield specifications for the iSeq 100 are as follows. | Read Length | Total Time | Output | Quality | | ----------- | ---------- | ------ | -------- | | 1x36 bp | 9 hrs | 144 Mb | >85% Q30 | | 1x50 bp | 9 hrs | 200 Mb | >85% Q30 | | 1x75 bp | 10 hrs | 300 Mb | >80% Q30 | | 2x75 bp | 13 hrs | 600 Mb | >80% Q30 | | 2x150 bp | 19 hrs | 1.2 Gb | >80% Q30 | Install specifications are based on the Illumina PhiX control library at supported cluster densities/occupancies. Total times include cluster generation, sequencing, and base calling with quality scoring on an iSeq 100 System. The current maximum supported read length is 150 bp for each insert read; however, the instrument will allow for run set up in any configuration as long as the total number of cycles used is fewer than 322 cycles. **Reads Passing filter** Single reads = 4 million Paired-end reads = 8 million For 100% PhiX runs, load double-stranded DNA at a final loading concentration of 60 pM. \ \ \
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