Specifications for the iSeq 100

Quality and yield specifications for the iSeq 100 are as follows.

Read Length	Total Time	Output	Quality
1x36 bp	9 hrs	144 Mb	>85% Q30
1x50 bp	9 hrs	200 Mb	>85% Q30
1x75 bp	10 hrs	300 Mb	>80% Q30
2x75 bp	13 hrs	600 Mb	>80% Q30
2x150 bp	19 hrs	1.2 Gb	>80% Q30

Install specifications are based on the Illumina PhiX control library at supported cluster densities/occupancies.

Total times include cluster generation, sequencing, and base calling with quality scoring on an iSeq 100 System.

The current maximum supported read length is 150 bp for each insert read; however, the instrument will allow for run set up in any configuration as long as the total number of cycles used is fewer than 322 cycles.

Reads Passing filter

Single reads = 4 million

Paired-end reads = 8 million

For 100% PhiX runs, load double-stranded DNA at a final loading concentration of 60 pM.

For any feedback or questions regarding this article (Illumina Knowledge Article #2117), contact Illumina Technical Support [e-mail].