Specifications for the iSeq 100

Quality and yield specifications for the iSeq 100 are as follows, and are available on the specifications page, here.

Read Length	Total Time	Output	Quality
1x36 bp	9 hrs	144 Mb	>85% Q30
1x50 bp	9 hrs	200 Mb	>85% Q30
1x75 bp	10 hrs	300 Mb	>80% Q30
2x75 bp	13 hrs	600 Mb	>80% Q30
2x150 bp	19 hrs	1.2 Gb	>80% Q30

Specifications are based on the Illumina PhiX control library at supported cluster densities/occupancies.

Total times include cluster generation, sequencing, and base calling with quality scoring on an iSeq 100 System.

The current maximum supported read length is 151 bp for each insert read; however, the instrument allows for run set up in any configuration as long as the total number of cycles used is fewer than 322 cycles.

Reads Passing filter

Single reads = 4 million

Paired-end reads = 8 million

For 100% PhiX runs, load double-stranded DNA at a final loading concentration of 100 pM.

For any feedback or questions regarding this article (Illumina Knowledge Article #2117), contact Illumina Technical Support [e-mail].