# Specifications for the iSeq 100

Quality and yield specifications for the iSeq 100 are as follows.

| Read Length | Total Time | Output | Quality  |
| ----------- | ---------- | ------ | -------- |
| 1x36 bp     | 9 hrs      | 144 Mb | >85% Q30 |
| 1x50 bp     | 9 hrs      | 200 Mb | >85% Q30 |
| 1x75 bp     | 10 hrs     | 300 Mb | >80% Q30 |
| 2x75 bp     | 13 hrs     | 600 Mb | >80% Q30 |
| 2x150 bp    | 19 hrs     | 1.2 Gb | >80% Q30 |

Install specifications are based on the Illumina PhiX control library at supported cluster densities/occupancies.

Total times include cluster generation, sequencing, and base calling with quality scoring on an iSeq 100 System.

The current maximum supported read length is 150 bp for each insert read; however, the instrument will allow for run set up in any configuration as long as the total number of cycles used is fewer than 322 cycles.

**Reads Passing filter**

Single reads = 4 million

Paired-end reads = 8 million

For 100% PhiX runs, load double-stranded DNA at a final loading concentration of 60 pM.

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| *For any feedback or questions regarding this article (Illumina Knowledge Article #2117), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000002117%20-%20Instrumentation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


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