RTA4 Q Score Bin Ranges on the MiSeq i100 Series
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Background
The MiSeq i100 Series utilizes Real Time Analysis v4 (RTA4) for base calling and quality scoring. There are three Q score bins used on the MiSeq i100 Series, and these Q score calls are reported as an average for the range of called scores. Please see below for the relevant Q Score Bins and Ranges.
Quality Score Bin
Quality Score Range
2
NoCall, 0-2
12
3-17
24
18-29
38
30+
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