RTA4 Q Score Bin Ranges on the MiSeq i100 Series

Background

The MiSeq i100 Series utilizes Real Time Analysis v4 (RTA4) for base calling and quality scoring. There are three Q score bins used on the MiSeq i100 Series, and these Q score calls are reported as an average for the range of called scores. Please see below for the relevant Q Score Bins and Ranges.

Quality Score Bin

Quality Score Range

2

NoCall, 0-2

12

3-17

24

18-29

38

30+

For any feedback or questions regarding this article (Illumina Knowledge Article #9540), contact Illumina Technical Support techsupport@illumina.com.

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