Reference Material

Library Preparation > General > Reference Material

• Adapter dimers的形成原因、影响及去除方法

• AmpliSeq for Illumina Dermatology Research Panel v2 product page, support page, and manifest links

• Amplicon Sequencing Introduction Support Webinar Video

• Bead handling best practices

• Best Practices for Ribo Zero Depletion using the TruSeq Stranded Total RNA Protocol

• Best practices for LNB1 handling in bead based normalization for TruSight Oncology 500 workflows

• Best practices for handling magnetic beads used with Illumina RNA library preparation kits

• Best practices for storage of bead normalized Nextera XT libraries

• Best practices for using Sodium Hydroxide with Illumina sequencing systems

• Choosing a Library Loading Concentration for Illumina Sequencing Video

• Custom primer requirements for the Illumina DNA PCR Free Prep, Tagmentation kit

• Fusion Genes kits for detection and lists of targeted genes

• How Do I Optimize Amplicon Sequencing Data? Part 1 Video

• How Do I Optimize Amplicon Sequencing Data? Part 2 Video

• How short inserts affect sequencing performance

• How to Use the Illumina Library Prep Kit Selector Video

• How to find a Safety Data Sheet

• How to use the Illumina Sequencing Coverage Calculator Video

• Illumina DNA Prep library preparation for the MiSeq i100 Series 25M Reagent Kit (1000 cycles)

• Index content for the TruSeq DNA and RNA Single Index kits, Sets A and B

• Indexed Sequencing Overview for Paired End Flow Cells

• Information on the Illumina Tagment DNA TDE1 Enzyme and Buffer Kits

• Input recommendations and considerations for Illumina DNA Prep with Enrichment (S) Tagmentation

• Library Prep Bead Handling Workflows Best Practices LNB1/2/3 (Video)

• Library Prep Bead Handling Workflows Best Practices SMB (Video)

• Library Prep Bead Handling Workflows Best Practices SPB/IPB Video

• Library denaturation and compatibility for the iSeq 100 system

• Metagenomics Part I Introduction to Library Preparation and Sequencing Support Webinar Video

• Optimal Cluster Density Best Practices Video

• PhiX Control v3文库是什么，它在Illumina下一代测序中起到什么作用？

• Plate layout for TruSeq Unique Dual (UD) indexes

• Product list and description for Illumina Rapid RNA kits

• Read Depth Requirements for Illumina Rapid RNA kits

• Recommendations for sequencing Illumina 16S libraries with the NextSeq 1000/2000 600 cycle kits

• Recommended quality control of FFPE samples for Illumina FFPE supported library preparation kits

• Resources for Illumina Rapid RNA kits

• Resources for getting started with Illumina Next Generation Sequencing (NGS)

• Respiratory Virus Enrichment Kit sequencing recommendations and public data resources

• Sequences to use for adapter trimming

• Sequencing FFPE Samples Introduction Support Webinar Video

• Sequencing Method Explorer：哪种二代测序（NGS）方法最适合我的研究？

• Spiking custom primers into the Illumina sequencing primers

• Understanding unique dual indexes (UDI) and associated library prep kits

• Using Sequencing Method Explorer to identify suitable NGS methods for research

• Why pooling libraries at the same concentration does not change the final pool concentration

• i7 and i5 indexes in each Nextera XT index kit

• 了解唯一双标签（UDI）与相关建库试剂盒

• 将定制引物掺入到Illumina测序引物中

• 手动文库浓度标准化最佳操作

• 是否可以混合不同类型的文库并同时进行测序？

• 最小化 TruSeq Stranded Total RNA 文库中rRNA污染的最佳操作

• 末端接头剪切（adapter trimming）时需要用到的序列