> For the complete documentation index, see [llms.txt](https://knowledge.illumina.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://knowledge.illumina.com/instrumentation/miniseq/instrumentation-miniseq-troubleshooting-list/000007359.md).

# Troubleshooting "Ebox0 Fan is not operating as expected" warnings on the MiniSeq

**Background**

The "Ebox0 Fan is not operating as expected" warning during runs on the MiniSeq does not impact sequencing quality. This warning may indicate a communications issue on the instrument or the need for maintenance on the fan itself.

![](https://761066130-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FGM9W2DuBTgEXv1ClCm8H%2Fuploads%2Fgit-blob-d501ec0a19ffbfa22d665523494b0068ae001fc1%2Fimage1.jpg?alt=media\&token=d894d031-805c-43e3-9a6b-9af706f2e299)

**Troubleshooting steps**:

1. Contact [Illumina Technical Support](https://www.illumina.com/company/contact-us.html#/united-states/technical-support) to document the issue.
2. Allow the run to complete and perform the post-run wash.
3. Power cycle the MiniSeq.
   1. For further information, see Illumina Knowledge Article [How to Power Cycle the MiniSeq](/instrumentation/miniseq/instrumentation-miniseq-reference_material-list/000002010.md).
   2. If the Control Software fails to initialize, contact [Illumina Technical Support](https://www.illumina.com/company/contact-us.html#/united-states/technical-support).
4. If the instrument initializes without errors, perform the Thermal Fan test through the MiniSeq System Checks.
   1. Navigate to Home screen in MiniSeq Control Software (MNCS).
   2. Select **Manage Instrument** --> **System Check**
   3. Follow the instructions to prepare the instrument for the check as indicated.
   4. Ensure only the following checks are selected:
      1. Thermal: **Fans**
5. If the system checks pass, this indicates the issue is related to a communications issue on the instrument and has been resolved. Proceed with the next run.
6. If the system checks fail, contact [Illumina Technical Support](https://www.illumina.com/company/contact-us.html#/united-states/technical-support) for further assistance.

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| *For any feedback or questions regarding this article (Illumina Knowledge Article #7359), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000007359%20-%20Instrumentation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


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