Troubleshooting BaseSpace connectivity issues on sequencing instruments

Uploading sequencing run data to Illumina BaseSpace Sequencing Hub requires an active internet connection. If an instrument is unable to connect to BaseSpace during a sequencing run or run the setup, see the following troubleshooting recommendations.

1. Power cycle the instrument according to the instrument System Guide.

2. Check the physical ethernet connection.

   1. Confirm the ethernet cable is plugged into the instrument and switch and is not damaged.

   2. If the ethernet port on the back of the instrument has an LED indicator light, verify that both are green.

3. Verify that the Date, Time, and Time Zone settings are correct.

   1. From the Windows Search bar, type in Control Panel and open the Control Panel App.

   2. Select 'Date and Time' settings.

   3. Confirm that the date, time, time zone, and daylight saving time settings are correct for the local region.

4. Check that the instrument has a valid IP address.

   1. In the Windows search bar, type in cmd and press Enter.

   2. In the Command Prompt window, type ipconfig and press enter.

   3. Check that the external facing network interface card (NIC) has a valid IP address.

   4. If there is an issue with the IP address, consult the local IT team.

5. Consult the local IT team to review the local Firewall and allow list settings.

   1. The required URLs are available here.

   2. Confirm that all websites listed under 'Regional Platform Endpoints' and the instrument-specific sections are on the Firewall allow list.

6. Consult the local IT and confirm that outbound ports 80, 443, and 8080 are open from the instrument to the Internet.

If issues with connecting to BaseSpace persist after these troubleshooting steps, contact Illumina Technical Support.

For any feedback or questions regarding this article (Illumina Knowledge Article #2250), contact Illumina Technical Support [e-mail].