# What is the difference between AmpliSeq for Illumina custom DNA HotSpot and Gene Design Panels?

What is the difference between AmpliSeq for Illumina custom DNA HotSpot and Gene Design Panels?

* A **Hotspot** Panel is used for targeting small SNPs that can be (each) targeted by a single amplicon. These designs include a single pool of panel primers (panel oligos).
* A **Gene** **Design** Panel is used for targeting genes or regions of the genome. These designs have at least two panel primer pools (panel oligo pools).

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| *For any feedback or questions regarding this article (Illumina Knowledge Article #1052), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000001052%20-%20Library%20Preparation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


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