Converting ng/µl to nM when calculating dsDNA library concentration
Some standard Illumina libraries, such as Nextera, require the use of dsDNA-specific fluorescent dye methods for accurate quantification. These methods typically measure dsDNA concentration in ng/µl. To convert from ng/µl to nM for cluster generation, follow the instructions below.
- 1.Determine the average size of the library by running it on an Agilent Technologies 2100 Bioanalyzer.
- 2.Use the following formula to convert from ng/μl to nM:
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