Converting ng/µl to nM when calculating dsDNA library concentration

Some standard Illumina libraries, such as Nextera, require the use of dsDNA-specific fluorescent dye methods for accurate quantification. These methods typically measure dsDNA concentration in ng/µl. To convert from ng/µl to nM for cluster generation, follow the instructions below.

1. Determine the average size of the library by running it on an Agilent Technologies 2100 Bioanalyzer.

2. Use the following formula to convert from ng/μl to nM:

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