# Troubleshooting iSeq 100 pre run check “Insufficient disk space/Not enough space on D:\” error

A sequencing run on the iSeq 100 requires about 2 GB of hard drive space.

The “Insufficient disk space.../ Not enough space on D:\” error can occur when there is insufficient remaining space on the D:\ drive. The most direct resolution is manually deleting unnecessary data from the D:. For the instrument "Temp" folder, this is done via Process Management within the Control Software. For a local output folder saved on the instrument, this is done via manual curation directly through the File Explorer window.

For further information, search for Knowledge Base article **How to clear hard drive space on the iSeq 100.**

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| *For any feedback or questions regarding this article (Illumina Knowledge Article #3184), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000003184%20-%20Instrumentation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


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