Troubleshooting iSeq 100 pre run check “Insufficient disk space/Not enough space on D:\” error

A sequencing run on the iSeq 100 requires about 2 GB of hard drive space.

The “Insufficient disk space.../ Not enough space on D:\” error can occur when there is insufficient remaining space on the D:\ drive. The most direct resolution is manually deleting unnecessary data from the D:. For the instrument "Temp" folder, this is done via Process Management within the Control Software. For a local output folder saved on the instrument, this is done via manual curation directly through the File Explorer window.

For further information, search for Knowledge Base article How to clear hard drive space on the iSeq 100.

For any feedback or questions regarding this article (Illumina Knowledge Article #3184), contact Illumina Technical Support [e-mail].