Thawing and Stability Guidelines for MiniSeq Reagent Kits
See the following for thawing and stability guidelines for MiniSeq reagent cartridges, flow cells, and hybridization buffer.
MiniSeq Reagent Cartridge (store at -20°C)
Thawing options:
Thaw at 37°C in a water bath for 35 minutes; the cartridge is stable for up to 2 hours.
Thaw at Room Temperature water bath for 90 minutes; the cartridge is stable for up to 24 hours if moved to 4°C.
Thaw at room temperature in air (no water bath) for 5 hours; the cartridge is stable for up to 24 hours if moved to 4°C.
Thaw at 2°C to 8°C (fridge) for 18 hours; the cartridge is stable for up to 72 hours at 4°C.
If reagents need to be stored during troubleshooting, store them at 4°C until ready to run within the stability time frames listed above.
MiniSeq flow cell (shipped dry, store 2-8°C until use)
Let sit for 15-30 minutes at room temperature prior to opening the package.
The flow cell can be returned to 4°C once if unopened.
Use flow cell within 12 hours once opened.
Flow cells are sensitive to moisture; clustering can be negatively impacted if the flow cell package is left open for too long prior to starting a run.
Hybridization Buffer (5 ml; store at -20°C)
Thaw at room temperature and store on ice until ready for use.
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