Thawing and Stability Guidelines for MiniSeq Reagent Kits

See the following for thawing and stability guidelines for MiniSeq reagent cartridges, flow cells, and hybridization buffer.

MiniSeq Reagent Cartridge (store at -20°C)

• Thawing options:

  • Thaw at 37°C in a water bath for 35 minutes; the cartridge is stable for up to 2 hours.

  • Thaw at Room Temperature water bath for 90 minutes; the cartridge is stable for up to 24 hours if moved to 4°C.

  • Thaw at room temperature in air (no water bath) for 5 hours; the cartridge is stable for up to 24 hours if moved to 4°C.

  • Thaw at 2°C to 8°C (fridge) for 18 hours; the cartridge is stable for up to 72 hours at 4°C.

• If reagents need to be stored during troubleshooting, store them at 4°C until ready to run within the stability time frames listed above.

MiniSeq flow cell (shipped dry, store 2-8°C until use)

• Let sit for 15-30 minutes at room temperature prior to opening the package.

• The flow cell can be returned to 4°C once if unopened.

• Use flow cell within 12 hours once opened.

  • Flow cells are sensitive to moisture; clustering can be negatively impacted if the flow cell package is left open for too long prior to starting a run.

Hybridization Buffer (5 ml; store at -20°C)

• Thaw at room temperature and store on ice until ready for use.

For any feedback or questions regarding this article (Illumina Knowledge Article #2004), contact Illumina Technical Support [email protected].