How to start a maintenance wash without used buffer cartridges on the NovaSeq 6000

If a wash needs to be performed without a used buffer cartridge, either prior to the first run or if no used buffer cartridge is available, perform a maintenance wash without the radio-frequency identification (RFID) scan via the NovaSeq Service Software.

Note 1: This will not clear the warning for performing a maintenance wash (wash flag) from NovaSeq Control Software. To clear the warning, contactIllumina Technical Support.

Note 2: Make sure everything is loaded for a wash, because washes will start immediately after selecting start.

Note 3: For required reagents and consumables to perform the Maintenance wash, search for Knowledge Base article Washing the NovaSeq 6000.

Procedure:

1. Close the NovaSeq Control Software.

2. Open the NovaSeq Service Software.

3. Select Save for the Output Repository page.

4. Select Fluidics on the main screen.
5. Navigate to the Recipes tab.

6. Start Maintenance Wash on one or both sides.

For any feedback or questions regarding this article (Illumina Knowledge Article #2512), contact Illumina Technical Support [e-mail].