Illumina Stranded mRNA Prep Ligation General FAQs
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What is the Illumina Stranded mRNA Prep, Ligation kit?
The Illumina Stranded mRNA Prep, Ligation kit constructs stranded mRNA libraries that are compatible with Illumina sequencing platforms. The workflow is based on oligo dT purification and targets polyadenylated RNA species (includes mRNA and non-coding RNA with polyadenylation) for library preparation.
Which species is the kit compatible with?
The kit is compatible with any species that has mRNA with polyadenylated 3’-ends.
Why choose to sequence mRNA instead of total RNA?
The choice of the application will depend on the project aim. mRNA selection will specifically target poly-adenylated RNA, while total RNA prepares RNA libraries based on rRNA depletion, targeting coding RNA and long non-coding RNA.
Which reagents are needed to complete the workflow?
The user guide lists the required Illumina kits and user-supplied consumables that are required for this application.
What do the anchors do?
The anchors are partial-adapter sequences. Once they are ligated, they serve as tags/priming sites for adding the index adapter sequences via PCR.
Can TruSeq RNA adapters be used with this kit?
No, this is not supported.
Which RNA molecules are targeted with this kit?
This kit will mainly target mRNA and long non-coding RNAs with polyadenylation. Small RNAs and non-polyadenylated transcripts will not be captured.
For any feedback or questions regarding this article (Illumina Knowledge Article #3151), contact Illumina Technical Support .