Humidity warning during pre run check on the NextSeq 1000/2000

The Humidity specification for the NextSeq 1000/2000 is 20-80% noncondensing relative humidity. If the humidity is outside of this range, the instrument will give a humidity warning.

Error: "Humidity is outside the expected range. Data quality may be affected. For more information, see the site prep guide."

The warning can be acknowledged and the sequencing run will start. However, Illumina cannot guarantee run performance will meet specifications if humidity is outside the specified operating range.

• Relative humidity level low < 20%: impacts fluidic delivery, increases static.

• Relative humidity level high > 80%: potential for condensation to impact imaging.

Work with the local facilities team to return the laboratory to the recommended operating specification.

See the NextSeq 1000/2000 Site Prep Guide for additional information.

For any feedback or questions regarding this article (Illumina Knowledge Article #3527), contact Illumina Technical Support [e-mail].