Sequencing primer compatibility and recommended library kits for the NextSeq 1000/2000 platforms

Illumina offers a wide variety of library preparation kits and sequencing platforms for different applications, each with its own coverage and indexing considerations. The table below lists the sequencing primers included in the NextSeq 1000/2000 cartridges, and the Illumina library preparation kits compatible with the NextSeq 1000/2000 platform. This information is based on the required data output and the indexing capabilities of each kit.

Note: The information in the table below is based on the required data output and indexing capabilities of each kit. Most Illumina libraries can be sequenced with all Illumina sequencing platforms. If a specific Illumina library is not listed below, contact Illumina Tech Support to discuss special considerations.

Refer to the Sequence Coverage Calculator and Library Prep and Array Kit Selector resources for additional information.

Table 1. Sequencing primer compatibility with Illumina library prep kits recommended for the NextSeq 1000/2000 sequencing platform.

For instructions on how to use custom primers on the NextSeq 1000/2000 platform, refer to the NextSeq 1000/2000 Custom Primer Guide.

For any feedback or questions regarding this article (Illumina Knowledge Article #5905), contact Illumina Technical Support [e-mail].