Pre Run Check Troubleshooting on the MiniSeq

If the MiniSeq pre-ruck checks do not pass, follow the guidance below for the check or checks that did not pass.

General/reagent checks

• Doors closed.

  • Make sure doors closed.

  • Power cycle* if issue persists.

• Consumables loaded.

  • Ensure consumables are properly loaded.

  • Power cycle* if issue persists

• Required Software.

  • Verify that the proper software/module is installed.

• Instrument and network disk space checks - the control software will provide a warning if the free storage is below 100Gb.

  • Clear disk space from the D:\ drive.

  • Clear disk space from the network output location, if needed.

• Network connection.

  • Confirms network status and physical network connection.

  • Check read/write permissions, availability of network location, and Ethernet plug.

Temperature

• Temperature ramp: checks function of flow cell heater plate.

  • Power cycle* and retry the pre-run checks.

• Temperature sensors**.

  • Power cycle* and retry the pre-run checks.

• Fans**

  • Power cycle* and retry the pre-run checks.

Imaging System

• Imaging limits**: moves stage to sensor limits.

  • Power cycle* and retry the pre-run checks.

• Z step-and-settle**: checks the ability of z-stage to move and stop moving in a set time.

  • Power cycle* and retry the pre-run checks.

• Bit Error rate: checks camera circuits.

  • Power cycle* and retry the pre-run checks.

• Flow cell registration**: checks if the flow cell is in the correct imaging position.

  • Clean and re-seat flow cell and check gaskets.

  • Power cycle* and retry the pre-run checks. If the checks do not pass, try a new flow cell.

Reagent Delivery

• Valve response**: tests the accuracy of valve and pump syringe rate movements.

  • Power cycle* and retry the pre-run checks.

• Pump: moves pump syringe to min and max positions.

  • Power cycle* and retry the pre-run checks.

Note: Pre-run checks will not check flow rates, so always visually inspect the flow cell gaskets prior to loading.

*For further information, search for Knowledge Base article How to Power Cycle the MiniSeq.

** These checks are also performed during the system check process.

If any pre-run check fails after a full power cycle, contact Illumina Technical Support.

For any feedback or questions regarding this article (Illumina Knowledge Article #1214), contact Illumina Technical Support [e-mail].