Are technical replicates needed for TruSeq Stranded RNA Seq experiments?

Technical (library preparation) replicates are generally not necessary for TruSeq Stranded Total and TruSeq Stranded mRNA kits, as the data are highly reproducible between repeated samples. Biological and other replicate needs depend on experimental design and data needs.

Technical replicates of FFPE tissue show high concordance, indicating robust library prep performance. Axes are log2 fragments per kilobase million (FPKM) gene expression plots. R2 value is shown.

For further information see the TruSeq Stranded mRNA and Total RNA data sheet.

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