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Troubleshooting Y Motor Errors on the MiSeq

The following MiSeq Y motor errors usually stop the run and indicate communication problems with the stage or problems with stage hardware:* Y Motor: Closed-loop move cannot reach target position: Start = 75.4150390625 mm, Target = 75.40472 mm, Distance = -0.0103190625000025 mm, Stopped at 74.42236328125 mm, ExceptionMessage =
  • The FPGA reported an error while executing command "YMVTO 154429"; error message: "Error: too many steps needed"
  • Error: Timeout of 60.000 seconds exceeded while waiting for FPGA command "YMVTILE 1327" to complete
Troubleshooting Steps:1. Perform the post-run wash, if the run stops or after the run is completed. 2. Power cycle* the MiSeq. 3. If no issues with re-initialization, perform the Y Stage test 5 times.
  • Make sure that a used flow cell is loaded on the instrument (the same wash flow cell should be okay).
  • Select Y Stage test.
    • MiSeq Control Software (MCS) v 4.0: From Main Menu, select System Check.
    • MCS 3.1 and older: From Welcome Screen, select Manage Instrument, select System Check.
  • Follow the prompts on the screen to start the test, and repeat the test 4 more times (5 times total).
  1. 4.
    If all Y Stage tests pass continue with normal sequencing.
  2. 5.
    If the system fails to initialize or any of the 5 replicates of the Y Stage test fail, contact Illumina Technical Support for further assistance.
For further information, search for the following Knowledge Base articles:* *How to Shut down or power cycle the MiSeq.
  • *How To Power Cycle the MiSeq Video.
  • System Checks on MiSeq.
For any feedback or questions regarding this article (Illumina Knowledge Article #1452), contact Illumina Technical Support [email protected].
Last modified 12d ago
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