# 双链DNA文库浓度的计算：从质量浓度(ng/µl)转化为摩尔浓度(nM)的换算公式
一些标准的illumina文库，例如Nextera文库，需要使用双链DNA特异的荧光染料法进行精确定量。 这些定量方法给出的浓度均是质量浓度(ng/µl，但是测序前的文库稀释实验用到的是摩尔浓度(nM)，因此我们需要将质量浓度(ng/µl) 转化为摩尔浓度(nM)。 请参考如下方法进行浓度换算：
1. 通过Agilent Technologies 2100 Bioanalyzer确定文库平均长度bp
2. 使用下方公式进行质量浓度和摩尔浓度的转换
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