Fusion Genes kits for detection and lists of targeted genes
Fusion genes arise through mutations such as translocations, insertions, and deletions, and have been implicated in a variety of human diseases.
Whole Transcriptome kits
Fusion genes can be detected with whole transcriptome sequencing, using one of the following kits:
While these kits differ in the library preparation strategies used, the resulting libraries all yield transcriptome information that can be analyzed for fusion gene detection.
Targeted sequencing kits (fixed panels)
Illumina also provides solutions for a targeted approach for analyzing specific gene fusions. The following kits are recommended to detect specific fusion genes:
- TruSight Oncology 500, TruSight Oncology 500 High-Throughput, and TruSight Oncology 500 ctDNA TruSight Oncology 500, TruSight Oncology 500 High-Throughput, and TruSight Oncology 500 ctDNA (for circulating tumor DNA or cell-free DNA) are enrichment-based assays that analyze 523 cancer-relevant genes from DNA and RNA. The DNA and RNA panels provide comprehensive coverage of biomarkers commonly mutated in numerous cancer types, including 55 genes for known and novel fusions and splice variants (See Table 3 for DNA content and Table 4 for RNA content in this data sheet). For TruSight Oncology 500 ctDNA, the gene list that includes 23 DNA fusions can be found here (under Product Literature).
- AmpliSeq for Illumina Panels fixed panels AmpliSeq for Illumina is a highly multiplexed PCR-based library preparation assay that uses oligonucleotides to amplify regions of interest to investigate SNVs, indels, CNVs, and gene fusions.
AmpliSeq for Illumina Ready-to-Use Panels that detect fusion genes are listed below.
Targeted sequencing kits (custom panels)
- The AmpliSeq for Illumina Custom RNA Fusion Panel enables detection of fusion genes and gene expression profiling for 12 to 1,200 gene targets in a single assay (human samples only). Targeted panels can be designed from over 1,000 well-annotated fusion genes and > 20,000 human RefSeq genes using the web-based assay design software tool, DesignStudio. Targets can be added via multiple options in DesignStudio, including add fusion:
- Another option, compatible with mouse, rat, and human samples, is the TruSeq Targeted RNA Expression kit, which is an extension-ligation-based workflow for gene expression profiling, variant screening, and analysis of isoforms/splice junctions. The assay targets are added by selecting predeveloped probes in the DesignStudio software. Under the Add targets section, select Gene Fusion, and add the target as gene1:gene2 (see below for an example). A gene fusion assay list can be downloaded here.