Reference Material

Library Preparation > RNA Library Prep > Reference Material

• Anchor ligation best practices for Illumina Stranded mRNA and Total RNA Prep Ligation workflows

• Assessing cDNA QC in Illumina Single Cell 3’ RNA Prep

• Assessing library QC in Illumina Single Cell 3’ RNA Prep

• Best Practices for Nuclei Isolation with alternative nuclei isolation protocols for Illumina Single Cell

• Best Practices for Ribo Zero Depletion using the TruSeq Stranded Total RNA Protocol

• Best practices for cell preparation for Illumina Single Cell prep

• Best practices for handling magnetic beads used with Illumina RNA library preparation kits

• Best practices for using Illumina Single Cell Nuclei Isolation Kits for Illumina Single Cell kits

• Best practices to minimize rRNA contamination in TruSeq Stranded Total RNA libraries

• Capture and wash best practices for Illumina RNA Prep with Enrichment (L) Tagmentation

• Cell input and volume loading recommendations for Illumina Single Cell 3’ RNA Prep

• Considerations for RNA Seq read length and coverage

• Differences between V4.0PLUS PIPseq and Illumina Single Cell (formerly PIPseq V)

• General best practices for Illumina RNA library preparation kits

• Illumina Single Cell 3' RNA Preparation library sequencing recommendations and guidelines

• Illumina Single Cell 3’ RNA Prep cDNA QC best practices

• Illumina Single Cell 3’ RNA Prep library QC best practices

• Library preparation options for sequencing the coronavirus SARS CoV 2 (associated with COVID 19)

• PhiX requirements for sequencing Illumina Stranded RNA libraries

• Post amplification clean up best practices for Illumina RNA Prep with Enrichment (L) Tagmentation

• Product page and support page for Illumina Stranded mRNA Prep, Ligation

• RNA Sequencing Part I Intro to Illumina's RNA Library Preparation Workflows Support Webinar Video

• RNA Sequencing Part II Best Practices for Illumina's RNA Prep Protocols Support Webinar Video

• RNA handling best practices for Illumina RNA library preparation kits

• RNA测序读长及测序深度的选择

• Representative cDNA QC traces for Illumina Single Cell 3’ RNA Prep

• Representative library QC traces for Illumina Single Cell 3’ RNA Prep

• Ribo Zero Plus depletion best practices

• Sample Enrichment Best Practices for Illumina Single Cell 3' RNA Prep

• Selecting cycle numbers for High and Low RNA sample types with Illumina Single Cell RNA prep kits

• Shelf life of Illumina Single Cell 3' RNA Prep kits

• Small RNA Seq Part I Best Practices Support Webinar Video

• Small RNA Seq Part II Best Practices Support Webinar Video

• Species compatibility table for Ribo Zero Human/Mouse/Rat and Ribo Zero Gold

• Species compatibility table for Ribo Zero Plant

• The importance of pilot studies for Illumina Single Cell RNA Preparation prior to workflow changes

• Types of small RNA and the need for a separate small RNA kit

• Which reads map to each strand in Stranded RNA workflows, and how is strandedness achieved?

• rRNA Depletion using Illumina Ribo Zero Plus