# Xp reagents components and stability on the NovaSeq 6000

NovaSeq 6000 Xp kits come in 2 configurations:

* **2 lane kits are for use with the S1 and S2 reagent kits.**
* **4 lane kits are for use with S4 kits.**

Each kit contains ExAmp reagents and a 2- or 4-lane manifold.

* **Note**: the reusable flow cell dock is sold separately.

**Flow Cell Dock**\
![](https://761066130-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FGM9W2DuBTgEXv1ClCm8H%2Fuploads%2Fgit-blob-297f13fd4840c093eb89636c555fbbab6396cfbc%2Fimage1.png?alt=media\&token=cf766355-02c8-4fba-8c17-7595dcd92dde)

* Xp flow cell dock is reusable.
* Flow cell is inverted and manifold is fitted over the flow cell.

  ![](https://761066130-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FGM9W2DuBTgEXv1ClCm8H%2Fuploads%2Fgit-blob-dc9afacb148b2c28b797330201b54b60bafb5567%2Fimage2.png?alt=media\&token=b99c0d90-5b3b-4407-884c-c72751df02ab)

**Xp Kit components, shipping conditions, and storage temperatures:**

| Quantity | Kit Component | Shipping Condition | Storage Temp                                                      |
| -------- | ------------- | ------------------ | ----------------------------------------------------------------- |
| 1        | DPX1/JPX1     | Dry ice            | -20°C                                                             |
| 1        | DPX2/JPX2     | Dry ice            | -20°C                                                             |
| 1        | DPX3          | Dry ice            | -20°C                                                             |
| 1        | Xp Manifold   | Dry ice            | \*Can leave frozen with the kit or store at room temperature (RT) |

* Thaw components at RT for 10 min then set aside on ice.
* Mix and use within 30 minutes at room temperature or 1 hour if placed on ice.
* If unmixed with library pool, Xp reagents can be refrozen once (freeze as quickly as possible after thawing).
* Extra mastermix should be discarded.
* Wells on the manifold are numbered right to left to match the lane number of an inverted flow cell.
* Magnetic clamp holds in place when on dock.

**Workflow**

* Start the run within 30 minutes after loading library pools on the flow cell.
  * **Note**: prolonged incubation might reduce the percentage of clusters passing filter (%PF).

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