Xp reagents components and stability on the NovaSeq 6000

NovaSeq 6000 Xp kits come in 2 configurations:

• 2 lane kits are for use with the S1 and S2 reagent kits.

• 4 lane kits are for use with S4 kits.

Each kit contains ExAmp reagents and a 2- or 4-lane manifold.

• Note: the reusable flow cell dock is sold separately.

Flow Cell Dock

• Xp flow cell dock is reusable.

• Flow cell is inverted and manifold is fitted over the flow cell.

  

Xp Kit components, shipping conditions, and storage temperatures:

Quantity	Kit Component	Shipping Condition	Storage Temp
1	DPX1/JPX1	Dry ice	-20°C
1	DPX2/JPX2	Dry ice	-20°C
1	DPX3	Dry ice	-20°C
1	Xp Manifold	Dry ice	*Can leave frozen with the kit or store at room temperature (RT)

• Thaw components at RT for 10 min then set aside on ice.

• Mix and use within 30 minutes at room temperature or 1 hour if placed on ice.

• If unmixed with library pool, Xp reagents can be refrozen once (freeze as quickly as possible after thawing).

• Extra mastermix should be discarded.

• Wells on the manifold are numbered right to left to match the lane number of an inverted flow cell.

• Magnetic clamp holds in place when on dock.

Workflow

• Start the run within 30 minutes after loading library pools on the flow cell.

  • Note: prolonged incubation might reduce the percentage of clusters passing filter (%PF).

For any feedback or questions regarding this article (Illumina Knowledge Article #2422), contact Illumina Technical Support [email protected].