The optimal raw cluster density range for a whole-genome library, such as PhiX, is 170-220k/mm2.
For a 100% PhiX run, Illumina recommends a loading concentration 1.4 pM to avoid over clustering the flow cell.
For further information, see Cluster Optimization Overview Guidearrow-up-right.
For any feedback or questions regarding this article (Illumina Knowledge Article #2008), contact Illumina Technical Support [email protected]envelope.
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