How does the NovaSeq 6000 Xp workflow differ from NovaSeq 6000 Standard workflow?

The NovaSeq 6000 Standard workflow allows a single library pool to be loaded across all lanes of the flow cell. In contrast, the NovaSeq 6000 Xp workflow enables the sequencing of different library pools in each lane of a NovaSeq 6000 flow cell. For the NovaSeq 6000 Xp workflow, the ExAmp/library mix is prepared manually and then loaded onto the flow cell using the NovaSeq 6000 Xp Flow Cell Dock.

The recommended loading concentrations for the Standard workflow are higher than for the Xp workflow. For more information on both workflows, refer to the Denature and Dilute Protocol Generator.

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