# Guidance for RNA isolated from PAXgene Blood RNA kit

Due to the presence of enzymatic inhibitors, RNA isolated using a PAXgene kit requires an additional cleanup step.

For total RNA isolated using a PAXgene Blood RNA kit or PAXgene Blood miRNA kit, do the following:

1. Purify the total RNA using one of the following procedures:
   1. Modified [RNeasy MinElute Cleanup Kit](https://www.qiagen.com/us/products/discovery-and-translational-research/dna-rna-purification/rna-purification/rna-clean-up/rneasy-minelute-cleanup-kit) as described in *Modified RNeasy MinElute Cleanup Kit* on page 18 of the [Globin-Zero Gold rRNA Removal Kit Reference Guide (Document# 15066013 v02).](https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/ribosomal-depletion/globin-zero-gold/globin-zero-gold-reference-guide-15066013-02.pdf)
   2. [RNA Clean and Concentrator-5](https://www.zymoresearch.com/collections/rna-clean-concentrator-kits-rcc/products/rna-clean-concentrator-5) as described in *RNA Clean & Concentrator-5 Kit* on page 20 of the [Globin-Zero Gold rRNA Removal Kit Reference Guide (Document# 15066013 v02).](https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/ribosomal-depletion/globin-zero-gold/globin-zero-gold-reference-guide-15066013-02.pdf)
2. Elute in 12 ul RNase-free water

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| *For any feedback or questions regarding this article (Illumina Knowledge Article #8483), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000008483%20-%20Library%20Preparation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


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