Guidance for RNA isolated from PAXgene Blood RNA kit
Due to the presence of enzymatic inhibitors, RNA isolated using a PAXgene kit requires an additional cleanup step.
For total RNA isolated using a PAXgene Blood RNA kit or PAXgene Blood miRNA kit, do the following:
Purify the total RNA using one of the following procedures:
Modified RNeasy MinElute Cleanup Kit as described in Modified RNeasy MinElute Cleanup Kit on page 18 of the Globin-Zero Gold rRNA Removal Kit Reference Guide (Document# 15066013 v02).
RNA Clean and Concentrator-5 as described in RNA Clean & Concentrator-5 Kit on page 20 of the Globin-Zero Gold rRNA Removal Kit Reference Guide (Document# 15066013 v02).
Elute in 12 ul RNase-free water
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