> For the complete documentation index, see [llms.txt](https://knowledge.illumina.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://knowledge.illumina.com/library-preparation/amplicon-library-prep/library-preparation-amplicon-library-prep-reference_material-list.md).

# Reference Material

Library Preparation > Amplicon Library Prep > Reference Material

* [16S library preparation for the MiSeq i100 Series 25M Reagent Kit (1000 cycles)](/library-preparation/amplicon-library-prep/library-preparation-amplicon-library-prep-reference_material-list/000010152.md)
* [Amplicon Sequencing Assessing the Success of Your Run Support Webinar Video](/library-preparation/amplicon-library-prep/library-preparation-amplicon-library-prep-reference_material-list/000008058.md)
* [How to use Illumina DesignStudio for Custom Sequencing Panels Video](/library-preparation/amplicon-library-prep/library-preparation-amplicon-library-prep-reference_material-list/000006951.md)
* [Recommendations for sequencing Illumina 16S libraries with the NextSeq 1000/2000 600 cycle kits](/library-preparation/amplicon-library-prep/library-preparation-amplicon-library-prep-reference_material-list/000007461.md)
* [What Does a Typical 16S Run Look Like on a MiSeq?](/library-preparation/amplicon-library-prep/library-preparation-amplicon-library-prep-reference_material-list/000007820.md)


---

# Agent Instructions
This documentation is published with GitBook. GitBook is the documentation platform designed so that both humans and AI agents can read, navigate, and reason over technical content effectively. Learn more at gitbook.com.

## Querying This Documentation
If you need additional information that is not directly available in this page, you can query the documentation dynamically by asking a question.

Perform an HTTP GET request on the current page URL with the `ask` query parameter, and the optional `goal` query parameter:

```
GET https://knowledge.illumina.com/library-preparation/amplicon-library-prep/library-preparation-amplicon-library-prep-reference_material-list.md?ask=<question>&goal=<endgoal>
```

`ask` is the immediate question: it should be specific, self-contained, and written in natural language.
`goal` is optional and describes the broader end goal you are ultimately trying to accomplish on behalf of the user. GitBook uses it to tailor the answer towards what is most useful for that goal.

The response will contain a direct answer to the question and relevant excerpts and sources from the documentation.

Use this mechanism when the answer is not explicitly present in the current page, you need clarification or additional context, or you want to retrieve related documentation sections.
