# How to troubleshoot “A component failed during sequencing” error messages on the NovaSeq 6000

NovaSeq 6000 "Component failed during sequencing" error messages can indicate an instrument communication issue or a hardware failure. This error may or may not call out a particular component. In some instances, the run can be restarted after power cycling the instrument.

![](https://761066130-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FGM9W2DuBTgEXv1ClCm8H%2Fuploads%2Fgit-blob-c676f6701a2a3de162cfea14c5cb6684a4f57685%2Fimage1.png?alt=media\&token=3efcb041-bba8-4ad7-a426-b20783407e69)

**Troubleshooting steps**:

**Note1**: This type of error can result from a BaseSpace connection issue. If a BaseSpace outage or connectivity issue is suspected, see [How to troubleshoot NovaSeq 6000 “A component failed during sequencing” error during a Cloud Outage](https://knowledge.illumina.com/instrumentation/novaseq-6000/instrumentation-novaseq-6000-troubleshooting-list/000009026).

**Error occurs within the first 10 minutes of starting**:

If this issue is observed at the very start of the sequencing run, the consumables can often be re-used, though it will be important to review the issue with Illumina Tech Support before attempting a repeat sequencing run. See [Troubleshooting "Component Failed" errors immediately after Pre-Run Checks on the NovaSeq 6000](https://knowledge.illumina.com/instrumentation/novaseq-6000/instrumentation-novaseq-6000-troubleshooting-list/000006548)

1. Confirm that the reagents are unpierced, and no run folder has been generated under C:\ProgramData\*\Illumina\NovaSeq\NovaSeqTemp.
2. If a run is being performed on the other side of the instrument and if the instrument is connected to Proactive, provide the Run ID to Illumina Technical Support by email (<techsupport@illumina.com>). If the instrument is not connected, collect logs listed under "Files to Collect" section below and email [Illumina Technical Support](https://www.illumina.com/company/contact-us.html#/united-states/technical-support). Cluster and SBS cartridges can be [refrozen](https://knowledge.illumina.com/instrumentation/general/instrumentation-general-reference_material-list/000001533) one time at -20°C.

**Error occurs mid-run:**

1. If the other side is idle, perform a full [power cycle](https://github.com/illumina-swi/illumina-knowledge/blob/master/articles/Knowledge/How-to-Power-Cycle-the-NovaSeq-6000-System/README.md) ([video format](https://knowledge.illumina.com/instrumentation/novaseq-6000/instrumentation-novaseq-6000-reference_material-list/000005611)) on the instrument. If the instrument initializes successfully, you can continue sequencing on the side that **did not** have an error.
2. To troubleshoot the side that had a component failure error, collect logs and email [Illumina Technical Support](https://www.illumina.com/company/contact-us.html#/united-states/technical-support).
3. If instructed by Technical Support to perform a [system check](https://github.com/illumina-swi/illumina-knowledge/blob/master/articles/Knowledge/How-to-perform-system-checks-on-the-NovSeq-6000/README.md) ([video format](https://knowledge.illumina.com/instrumentation/novaseq-6000/instrumentation-novaseq-6000-reference_material-list/000005620)), follow the video instructions.

**Files to collect:**

1. Cycle logs: C:\ProgramData\*\Illumina\NovaSeq\NovaSeqTemp\[run folder]\Logs (zipped)
2. Control Software Logs: C:\ProgramData\*\Illumina\NovaSeq\NovaSeq Control Software\Logs (zipped)

\*Note: The **ProgramData** folder may be hidden. To unhide it, select the **View** tab in the file explorer window and make sure that **Hidden items** is checked.

After the logs have been collected, contact [Illumina Technical Support](https://www.illumina.com/company/contact-us.html#/united-states/technical-support) (<techsupport@illumina.com>) to determine the next steps.

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| *For any feedback or questions regarding this article (Illumina Knowledge Article #1265), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000005620%20-%20Instrumentation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |