Troubleshooting Cavro Pump Error on MiSeq

A Cavro Pump error can result from an issue with the instrument hardware or communication errors on the MiSeq. Potential hardware related causes include pump, pump controller, or syringe failure.

Error: Cavro pump error: Volume will exceed syringe capcity.Error in LanePump. Invalid argument. Command = '1V400IP1500R\r', reply = 'c'

Troubleshooting Steps:

1. Power cycle\* the MiSeq. (Note: If a run is in progress, it will not be able to be resumed.)

2. If there are no issues with re-initialization:

• Perform post-run wash to ensure fluidics is operating normally.

• Optionally, perform a volume test within the system check.

1. If the instrument fails to initialize or there is an error completing the wash, contact Illumina Technical Support for further assistance.

For further information, search for the following Knowledge Base articles:* *\How to Shut down or power cycle the MiSeq.

• *\How To Power Cycle the MiSeq Video.

• System Checks for MiSeq.

• Troubleshooting Lane Pump Errors on the MiSeq.

For any feedback or questions regarding this article (Illumina Knowledge Article #1454), contact Illumina Technical Support [email protected].