# Index read strategy for the NextSeq 1000/2000

The NextSeq 1000/2000 uses the **reverse complement workflow**, similar to the NextSeq 500/550 and MiniSeq. A solution-based index 2 sequencing primer is used, as illustrated below.

![](https://761066130-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FGM9W2DuBTgEXv1ClCm8H%2Fuploads%2Fgit-blob-0c12860a65980555cdb63fe3ebc7e23c7cddb913%2Fimage1.png?alt=media)

Refer to the [Indexed Sequencing Overview Guide](https://support.illumina.com/downloads/indexed-sequencing-overview-15057455.html) for more information.

\
\
\ <br>

|                                                                                                                                                                                                                                                                                                                                                                        |
| :--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------: |
| *For any feedback or questions regarding this article (Illumina Knowledge Article #2434), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000002434%20-%20Instrumentation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |