Index read strategy for the NextSeq 1000/2000

The NextSeq 1000/2000 uses the reverse complement workflow, similar to the NextSeq 500/550 and MiniSeq. A solution-based index 2 sequencing primer is used, as illustrated below.

Refer to the Indexed Sequencing Overview Guide for more information.

For any feedback or questions regarding this article (Illumina Knowledge Article #2434), contact Illumina Technical Support [e-mail].