Sequencing FAQs for Illumina Stranded Total RNA Prep Ligation with Ribo Zero Plus

What are the advantages of performing paired-end sequencing of RNA libraries? Paired-end sequencing can help with data alignment, discovery of novel transcripts, and identification of PCR duplicates.

What are the suggested loading concentrations for the various sequencers? Follow the guidelines in the specific reference guides of the relevant instrument.

Which strand is sequenced in Read 1? Read 1 sequences will be identical to the antisense strand of DNA, which is also the first strand cDNA (only the first strand cDNA is amplified and sequenced).

Can libraries prepared with this kit be combined with other library types in the same flow cell lane? This is not supported.

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