# Sequencing FAQs for Illumina Stranded Total RNA Prep Ligation with Ribo Zero Plus

**What are the advantages of performing paired-end sequencing of RNA libraries?**\
Paired-end sequencing can help with data alignment, discovery of novel transcripts, and identification of PCR duplicates.

**What are the suggested loading concentrations for the various sequencers?**\
Follow the guidelines in the specific reference guides of the relevant instrument.

**Which strand is sequenced in Read 1?**\
Read 1 sequences will be identical to the antisense strand of DNA, which is also the first strand cDNA (only the first strand cDNA is amplified and sequenced).

**Can libraries prepared with this kit be combined with other library types in the same flow cell lane?**\
This is not supported.

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