# General information regarding RNA samples in the TruSight Oncology 500 (TSO500) workflow

**What is the protocol for preparing RNA samples in the TSO500 workflow?**\
Refer to the [TSO500 reference guide](https://support.illumina.com/downloads/trusight-oncology-500-reference-guide-1000000067621.html) for the protocol; both the DNA and RNA protocols are included in this guide.

**Are the UMI adapters also used for RNA samples?**\
No, they are not; UMI adapters are used for DNA samples only.

**Is the RNA content in the TSO500 DNA+RNA the same as the TruSight Tumor 170 (TST170) RNA content?**\
Yes, TSO500 uses the TST170 RNA probe panel, OPR1.

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| *For any feedback or questions regarding this article (Illumina Knowledge Article #3171), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000003171%20-%20Library%20Preparation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


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