# How to Restart File Transfer on the MiSeq i100 Series

## **Background**

The MiSeq i100 Series instrument uses Universal Copy Service (UCS) to automatically copy data from the instrument to a configured External Storage location. If transfer must be restarted, for example, transferring the data to a different External Storage location or if there is an issue with the initial data transfer, data copy can be restarted from within the Control Software.

See the following instructions for using the Restart File transfer workflow on the MiSeq i100 Series instruments.

**IMPORTANT**: If using the **Restart file transfer** process to transfer files to **USB External Storage** such as an external hard drive or a flash drive, see section *Verifying Complete Data Copy* below.

## **Resolution Actions**

If transferring to a new External Storage or Output folder location, see the [Product Documentation - External Storage](https://support-docs.illumina.com/IN/MiSeqi100Series/Content/IN/MiSeqi100/SysOutFolder.htm) instructions for assistance with setting up the new External Storage and Output folder.

1. From the MiSeq i100 Series Control Software Home screen, select the dropdown menu in the top-left corner, then select **Runs**.
2. In the Runs user interface (UI), select the **Completed** tab.
3. Under the Run Name column, select the **sequencing run** to open the Run Details UI.
4. In the Run Details UI, select the **Show More** dropdown next to **Run Status**.
5. Select **Restart file transfer**.
6. Use the **Copy output location** dropdown to select the desired location from the list of configured output folder locations.
7. Select **Restart**.

**Verifying Complete Data Copy for USB Storage Devices**

Once the Control Software indicates data copy is complete, wait **at least 1 hour** to ensure that Linux Operating System (OS) has completed the data copy.

After 1 hour, confirm that the data copy is complete using the following steps:

1. From the Control Software Home screen, select the **dropdown menu** in the top-left corner, then select **Settings**.
2. Select **External Storage**.
3. Locate the USB external storage device in the External Storage user interface (UI), then select **Eject**.
4. **DO NOT** remove the USB external storage device from the instrument.
5. Select **Add USB Storage** and follow the prompts to re-mount the USB external device.
   1. If successful, select **Eject** in the External Storage UI and then **remove** the USB external storage device.
   2. If a **Bad Request** error presents, wait 15-20 minutes and attempt to re-mount the USB external storage device again. If successful, Eject and remove the USB external storage device.

If there are any issues with performing the restart file transfer workflow, contact [Illumina Technical Support](https://www.illumina.com/company/contact-us.html) for assistance.

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| *For any feedback or questions regarding this article (Illumina Knowledge Article #9335), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000009335%20-%20Instrumentation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


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