Can a NextSeq 1000/2000 sequencing run be set up with fewer than 26 cycles in read 1?

Yes, it is possible to enter fewer than 26 cycles in read 1 from local run setup. The NextSeq 1000/2000 control software will display a warning that sequencing 26 cycles or more in read 1 is recommended for accurate calculation of sequencing metrics. Acknowledge the warning and continue with consumable loading.

Illumina does not support runs with fewer than 26 cycles for read 1.

For any feedback or questions regarding this article (Illumina Knowledge Article #6385), contact Illumina Technical Support [e-mail].