# Can a NextSeq 1000/2000 sequencing run be set up with fewer than 26 cycles in read 1?

Yes, it is possible to enter fewer than 26 cycles in read 1 from local run setup. The NextSeq 1000/2000 control software will display a warning that sequencing 26 cycles or more in read 1 is recommended for accurate calculation of sequencing metrics. Acknowledge the warning and continue with consumable loading.

Illumina does not support runs with fewer than 26 cycles for read 1.

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| *For any feedback or questions regarding this article (Illumina Knowledge Article #6385), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000006385%20-%20Instrumentation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


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