# Differences with TruSeq Stranded RNA LS and HS protocols and single and dual index adapters

**What are the protocol differences when using single index and dual index adapters?**

The volume of purification beads differ in the post-PCR clean-up step. This difference is to account for the difference in adapter lengths between single and dual indexes.\
**What's the difference between the HS (high sample) protocol and the LS (low sample) protocol?**\
The HS protocol requires additional peripheral equipment but reduces user touch-points and is ideally suited for batches with higher sample numbers. The LS protocol requires minimal peripheral equipment but requires more user manipulation and is best suited for lower sample number batches. For specific differences between these workflows refer to the reference guide.

**Can dual index adapters be used with the LS protocol? Can single index adapters be used with the HS protocol?**\
Yes, the LS and HS workflows may be used when using the single or dual index adapters.

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| *For any feedback or questions regarding this article (Illumina Knowledge Article #1149), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000001149%20-%20Library%20Preparation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


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