How to troubleshoot Reagent Selector Valve Errors on the NovaSeq 6000

Reagent Selector Valve errors can be caused either due to a transient instrument communication issue or an instrument hardware related issue.* Run stopping error messages:

• "The software has exceeded three attempts to correct a problem with the reagent selector valve, and cannot save the flow cell. Call Technical Support for help."

• "The software has exceeded three attempts to correct a problem with the reagent selector valve and must end the sequencing run. Call Technical Support for help."

To troubleshoot this type of error:

• Power cycle the instrument. For more information on the power cycle, search for Knowledge Base article How to Power Cycle the NovaSeq 6000 System?

• Perform Valve Control test under Fluidics system check 5 times.

  • For more information on system check, search for Knowledge Base article How to perform system check the NovaSeq 6000 System?

  • If any of the 5 system check for these tests fail, contact Illumina Technical Support with system check results for the next steps.

  • If all tests pass, perform a wash and monitor for any valve error on the next run.

  • If the next run fails with the same error, contact Illumina Technical Support.

For any feedback or questions regarding this article (Illumina Knowledge Article #1302), contact Illumina Technical Support [email protected].