Troubleshooting Network errors during run setup on the NovaSeq 6000

​​​​​​The following types of network errors can occur during run setup on the NovaSeq 6000.

1. Unable to browse to the output folder location

The NovaSeq 6000 platform requires the designation of an output folder path to a network location prior to the start of a sequencing run in Manual mode. For more information on how to configure the output folder path in File-Based mode, refer to the NovaSeq 6000 Sequencing System Guide.

Important Notes:

• If the run uses BaseSpace Sequencing Hub for both analysis and storage, setting a network output folder location is optional.

• A mapped network drive should be configured by a network administrator.

• The Universal Name Conversion (UNC) path for the network drive is required for setting the output location. Using a mapped letter, such as Y:, results in an Invalid Drive error message, and will not work.

• Example of a UNC path: \servername\share-directory

To specify a default output location in the NovaSeq Control Software:

1. From the Main Menu, select Settings.

2. Select Manual.

3. Select Browse (next to the Output Folder field).

4. Type or copy and paste the UNC path into the address bar.

5. Select Enter on the keyboard, and input network credentials if prompted.

6. Designate the desired folder and select Select Folder.

7. Select Save.

Once the default output location is designated, the output folder field will automatically populate on the Run Setup screen for future runs. To change the output location for a single run, follow steps 3-7 on the Run Setup screen.

For additional information on networking requirements or run setup procedures, refer to the following resources.

NovaSeq 6000 System Guide NovaSeq 6000 Site Prep Guide

2. Unable to verify the network connection. Check that all network cables are connected and retry. This error can occur during pre-run checks.

• Confirm the network drive is connected in File Explorer and verify that the correct network UNC path was used.

  • The UNC path for the network drive is required for setting the output location. Using a mapped letter, such as Y:, results in an Invalid Drive error message.

  • Example of a UNC path: \servername\share-directory

• Note: As a workaround, the run can be saved to an external drive, but data transfer times may take longer.

• Make sure there is sufficient space locally. Clear runs via Process Management in NovaSeq Control Software (NVCS).

• Verify that there is sufficient space in the output folder. Delete older runs from the output folder location.

• Contact Illumina Technical Support if the issue persists.

For any feedback or questions regarding this article (Illumina Knowledge Article #1287), contact Illumina Technical Support [e-mail].