How to resolve an error occurred while populating NextSeq 1000/2000 data in SAV

Background: Sequencing Analysis Viewer (SAV) is a software application that enables viewing of quality metrics generated by the Real-Time Analysis (RTA) software on Illumina sequencing systems. The NextSeq 1000/2000 Control Software release v1.5 requires SAV version 2.5.12 or later to view run metrics. If visualizing data generated from NextSeq 1000/2000 Control Software version 1.5 in SAV version 2.4 user will encounter an error indicating inability to parse various InterOp folder files.

SAV v2.5.12 is compatible with runs generated in the newer NextSeq 1000/2000 Control Software (v1.7.1) for Standard reagents only. If running XLEAP kits, it will show the same errors.

Examples of error language include:

• An error occurred while populating the data: No format found to parse ErrorMetricsOut.bin with version 6 of 3

• An error occurred while populating the data: No format found to parse BasecallingMetricsOut.bin with version 5 of 3

  

Troubleshooting:

• If on SAV v2.4: Update SAV to v2.5.12 - SAV download can be found on the NextSeq 1000/2000 Software Downloads page.

• If on SAV v2.5.12 trying to analyze XLEAP runs: Update SAV to v3.0 or move the "BasecallingMetricsOut.bin" and the "RegistrationMetricsOut.bin" files from the InterOp folder to another folder (we don't recommend deleting those as they might be necessary in case of troubleshooting).

For any feedback or questions regarding this article (Illumina Knowledge Article #7427), contact Illumina Technical Support [e-mail].