NovaSeq 6000 storage management and best practices

Before each NovaSeq 6000 run:

1. Verify data transfer completion from previous runs:

   1. In the NovaSeq 6000 Control Software, from the top left menu, select Process Management and verify the previous run status is “Completed” with a green mark.

2. Delete all completed and successfully transferred runs:

   1. In the NovaSeq 6000 Control Software, from the top left menu, select Process Management and delete all runs with the status “Completed” with a green mark.

3. Verify the output folder is selected in the correct format:

   1. In the NovaSeq 6000 Control Software, from the top left menu, select Settings and verify the output folder is selected and entered in the Universal Naming Convention (UNC) format.Do not use Browse to select the output folder.

   2. UNC formatting example: \111.222.333\Runs\Jul-Aug

   3. The command net use can be used in Windows Command Prompt to find all network destinations in UNC format.

4. Verify disk space using Windows File Explorer by pressing the windows key + E on keyboard, then selecting “This PC” and check:

   1. D:\ should have at least 8 GB of space.

   2. For C:\ and Z:\ drives, see Knowledge Base article Minimum storage requirements to start a NovaSeq 6000 run for specific details on minimum space requirements.

For any feedback or questions regarding this article (Illumina Knowledge Article #3495), contact Illumina Technical Support [e-mail].