NovaSeq 6000 storage management and best practices

Before each NovaSeq 6000 run:

1. Verify data transfer completion from previous runs: In the NovaSeq Control Software, select Process Management (top left menu) and check that the previous run status is “Completed” with a green mark.

2. Delete all completed and successfully transferred runs: In the NovaSeq Control Software, select Process Management (top left menu) and delete all runs with the status “Completed” with a green mark.

3. Verify the output folder is selected in the correct format: In the NovaSeq Control Software, select Settings (top let menu) and verify that the correct output folder is selected and entered in the UNC format. Do not use “Browse” to select the output folder. UNC formatting example: \111.222.333\Runs\Jul-Aug. Net Use command can be used in command prompt to find all network destinations in UNC format.

4. Verify disk spaces: In Windows File Explorer (windows+E on keyboard), select “This PC” and check: D:\ should have at least 8 GB of space. For C:\ and Z:\ the more free space the better, check point 2, above, to free space. For more information, search for Knowledge Base article What are the minimum storage requirements to start a NovaSeq 6000 run?

For any feedback or questions regarding this article (Illumina Knowledge Article #3495), contact Illumina Technical Support [e-mail].