# Technical resources for the MiSeq

A comprehensive list of resources needed to set up, operate, maintain, and troubleshoot the MiSeq sequencing system is on the MiSeq Support page. Specific documentation links are found below.

[MiSeq Sequencing Welcome Card](https://support.illumina.com/content/dam/illumina-support/documents/documentation/system_documentation/miseq/qrc/welcome_to_illumina_miseq_sequencing_15044362_a.pdf)\
Graphical introduction to the MiSeq System.

[MiSeq Sample Sheet Quick Reference Guide](https://support.illumina.com/downloads/miseq_sample_sheet_quick_reference_guide_15028392.html)\
Guidance for setting up sample sheets for sequencing on the MiSeq.

[MiSeq Run Folder Quick Reference Card](https://support.illumina.com/content/dam/illumina-support/documents/documentation/system_documentation/miseq/miseq-output-and-analysis-folders-qrc-15034791-c.pdf)\
Instructions for using the MiSeq output and analysis folders.

[MiSeq System Specification Sheet](https://www.illumina.com/content/dam/illumina/gcs/assembled-assets/marketing-literature/miseq-system-data-sheet-m-gl-00006/miseq-data-sheet-m-gl-00006.pdf)\
Contains basic information including output and time specifications for the MiSeq.

[MiSeq System Specification Page](https://www.illumina.com/systems/sequencing-platforms/miseq/specifications.html)\
Provides the data quality and yield specifications for the MiSeq.

[MiSeq Sequencing System Site Prep Guide](https://support.illumina.com/downloads/miseq_site_prep_guide_15027615.html)\
Contains lab requirements, networking guidelines, and security considerations.

[MiSeq System Guide for MiSeq Reporter](https://support.illumina.com/downloads/miseq-system-user-guide-for-miseq-reporter-1000000061014.html)\
Contains a workflow overview, maintenance procedures, and troubleshooting resources for MiSeq systems running MCS v2.6 and below.

[MiSeq System Guide for Local Run Manager](https://support.illumina.com/downloads/miseq_system_user_guide_15027617.html)\
Contains a workflow overview, maintenance procedures, and troubleshooting resources for MiSeq systems running MCS v3.1 and greater.

[Local Run Manager Support Resources](https://support.illumina.com/sequencing/sequencing_software/local-run-manager.html)\
Contains on- and off-instrument versions of Local Run Manager.

[MiSeq System Custom Primers Guide](https://support.illumina.com/downloads/miseq-system-custom-primers-guide-15041638.html)\
Contains instructions for preparing and loading custom primers and editing the sample sheet when using custom primers on a MiSeq run.

[MiSeq System Denature and Dilute Libraries Guide](https://support.illumina.com/downloads/prepare_libraries_for_sequencing_miseq_15039740.html)\
Contains instructions for preparing PhiX and denaturing and diluting libraries for sequencing on the MiSeq.

[Cluster Optimization Overview Guide](https://support.illumina.com/downloads/cluster-optimization-overview-guide-1000000071511.html)\
Describes strategies for optimizing cluster density and preventing and diagnosing clustering issues on Illumina flow cells.

[Online Training Courses](https://support.illumina.com/sequencing/sequencing_instruments/miseq/training.html?langsel=/us/)\
Online trainings and recorded webinars include

* video courses on preparing for instrument installation
* how to start a run
* how to perform instrument washes
* how to set up / view analysis results on Local Run Manager

To access additional online training courses, navigate to the [Training website](https://support.illumina.com/training.html), and under “Filters” select **Training Type** > **Training and Videos** > **Online Training**

[Safety Data Sheets](https://support.illumina.com/sds.html)\
Safety Data Sheets contain information to help with appropriate reagent disposal practices. To obtain this information, enter the kit catalog number, language, and region/country, then select **Search**.

Additional technical resources are available 24/7 at the [MiSeq Sequencing System Support website](https://support.illumina.com/sequencing/sequencing_instruments/miseq.html).

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| *For any feedback or questions regarding this article (Illumina Knowledge Article #7486), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000007486%20-%20Instrumentation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |