Technical resources for the MiSeq
A comprehensive list of resources needed to set up, operate, maintain, and troubleshoot the MiSeq sequencing system is on the MiSeq Support page. Specific documentation links are found below.
MiSeq Sample Sheet Quick Reference Guide
Guidance for setting up sample sheets for sequencing on the MiSeq.
MiSeq Run Folder Quick Reference Card
Instructions for using the MiSeq output and analysis folders.
MiSeq System Specification Sheet
Contains basic information including output and time specifications for the MiSeq.
MiSeq Sequencing System Site Prep Guide
Contains lab requirements, networking guidelines, and security considerations.
MiSeq System Guide for MiSeq Reporter
Contains a workflow overview, maintenance procedures, and troubleshooting resources for MiSeq systems running MCS v2.6 and below.
MiSeq System Guide for Local Run Manager
Contains a workflow overview, maintenance procedures, and troubleshooting resources for MiSeq systems running MCS v3.1 and greater.
MiSeq System Custom Primers Guide
Contains instructions for preparing and loading custom primers and editing the sample sheet when using custom primers on a MiSeq run.
MiSeq System Denature and Dilute Libraries Guide
Contains instructions for preparing PhiX and denaturing and diluting libraries for sequencing on the MiSeq.
Cluster Optimization Overview Guide
Describes strategies for optimizing cluster density and preventing and diagnosing clustering issues on Illumina flow cells.
Low-Diversity Sequencing on the Illumina MiSeq Platform
Describes changes when using RTA version 1.17.28 with low diversity samples in newer versions of MiSeq Control Software (MCS).
MiSeq System Frequently Asked Questions
Answers to commonly asked questions about running on a MiSeq system.
- video courses on preparing for instrument installation
- how to start a run
- how to perform instrument washes
- how to set up / view analysis results on Local Run Manager
To access additional online training courses, navigate to the Training website, and under Filters select Training Type > Training and Videos > Online Training
Safety Data Sheets
Safety Data Sheets contain information to help with appropriate reagent disposal practices. To obtain this information, enter the kit catalog number, language, and region/country, then select Search.
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