# Thawing, stability, and storage of NovaSeq 6000 reagents

For information regarding the thawing, stability, and storage of NovaSeq 6000 reagents, see below.

Reagents in the SBS and buffer cartridges are sensitive to light. Protect those cartridges from light by storing them in a dark location away from any light sources.

**Temperature key**

| Storage Condition     | Temperature range °C |
| --------------------- | -------------------- |
| Room temperature (RT) | 15°C to 30°C         |
| Refrigerator (4°C)    | 2°C to 8°C           |
| Freezer (-20°C)       | -25°C to -15°C       |

**Kit Components, shipping conditions, and storage temperatures**

| Quantity | Kit Component     | Shipping Condition | Storage Temp   | Notes                                                                                                                                                                                       |
| -------- | ----------------- | ------------------ | -------------- | ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- |
| 1        | Library tube      | Room temp          | 15°C to 30°C   |                                                                                                                                                                                             |
| 1        | Buffer cartridge  | Room temp          | 15°C to 30°C   | Light sensitive.                                                                                                                                                                            |
| 1        | Flow cell         | Ice pack           | 2°C to 8°C     | <p>Before use, allow the flow cell to sit at room temperature for 10-15 minutes before removing from the packaging.<br><br>Once open, use within 12 hours of removing from the package.</p> |
| 1        | Cluster cartridge | Dry ice            | -25°C to -15°C |                                                                                                                                                                                             |
| 1        | SBS Cartridge     | Dry ice            | -25°C to -15°C | Light sensitive.                                                                                                                                                                            |

**Thawing guidelines for each kit component**

For the components that are thawed in a water bath, place them in a room temperature water bath (19°C - 25°C) following the timing guidance specified below.

**Cluster cartridges** \*Shipped with dry ice (store at -25°C to -15°C).

| Cluster cartridge type | Duration of thaw                                                   |
| ---------------------- | ------------------------------------------------------------------ |
| SP, S1, S2             | Up to 2 hours in a room temperature (RT) water bath (19°C - 25°C). |
| S4                     | Up to 4 hours in room temperature (RT) water bath (19°C - 25°C).   |

* Use a wire rack to prevent tipping.
* If reagents cannot be loaded onto the instrument within 4 hours, store at 2°C to 8°C for up to 24 hours.
* Can refreeze once, if needed. If refreezing, do so immediately after thawing.

**SBS cartridges** \*Shipped with dry ice (store at -25°C to -15°C).

| SBS cartridge type | Duration of thaw                                          |
| ------------------ | --------------------------------------------------------- |
| SP, S1, S2         | 4 hours in room temperature (RT) water bath (19°C - 25°C) |
| S4                 | 4 hours in room temperature (RT) water bath (19°C - 25°C) |

* Use a wire rack to prevent tipping.
* If reagents cannot be loaded onto the instrument within 4 hours, store at 2°C to 8°C for up to 24 hours.
* Can refreeze once, if needed. If refreezing, do so immediately after thawing.
* SBS cartridges are sensitive to light.

**Buffer Cartridge** \*Shipped at RT (store at RT).

* Buffer cartridges are sensitive to light.
* Buffer cartridges can be recycled (only consumable that can be recycled).

**Flow cell (FC)** \*Shipped dry with an ice pack (store at 2-8°C).

* Before use, allow the flow cell to sit at room temperature for 10-15 minutes before removing it from the packaging.
* Once open, use within 12 hours of removing it from the package.

**Library tube** \*Shipped at RT (store at RT).

* Store the library tube (containing the library pool) only if necessary.
* Long-term library pool storage at -25°C to -15°C can increase duplicates, which decreases yield.

For further information, search for Knowledge Base article **Xp reagents components and stability on the NovaSeq 6000** and **How to thaw and store sequencing reagents for optimal performance.**

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| *For any feedback or questions regarding this article (Illumina Knowledge Article #2421), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000002421%20-%20Instrumentation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


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