> For the complete documentation index, see [llms.txt](https://knowledge.illumina.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://knowledge.illumina.com/instrumentation/nextseq-1000-2000/instrumentation-nextseq-1000-2000-troubleshooting-list/000007029.md).

# Troubleshooting NextSeq 1000/2000 Control Software failure to initialize with cartridge error

**Background and Potential Causes**: This error can occur if the instrument is improperly power cycled with the reagent cartridge and flow cell still inside the instrument. Upon restarting the control software, it can fail to initialize.

**Error Message:** SystemStartupException Initialization failed on Cartridge Interface Module

**Troubleshooting steps:**

* Perform a full, complete power cycle.
  * For additional information, search for Knowledge Base Article [How to power cycle the NextSeq 1000/2000](https://knowledge.illumina.com/instrumentation/nextseq-1000-2000/instrumentation-nextseq-1000-2000-reference_material-list/000003038).
* If the error recurs after the power cycle, contact [Illumina Technical Support](https://www.illumina.com/company/contact-us.html#/united-states/technical-support).

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| *For any feedback or questions regarding this article (Illumina Knowledge Article #7029), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000007029%20-%20Instrumentation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


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