Internal network issues on the NextSeq 1000/2000

Internal network issues on the NextSeq 1000/2000 can result in the following.

• NextSeq 1000/2000 Control Software that is unresponsive, slow, or lagging.

• Initialization failure after power cycling with a timeout error.

Note: If the system has recently undergone networking to a network drive, it is possible the internal network settings may have been changed.

The error may also present in the form of initialization failure with an error that references a failure to connect to the client or server.

To resolve this type of error, check the internal Network Interface Card (NIC) settings:

• If logged in ilmnuser account, switch accounts to ilmnadmin account to make changes to the system settings.

• From Applications, select System Tools, select Settings, andselect Network.

• Select Settings for enp5s0 (this is the internal NIC).

  • enp2s0 is the external NIC, if this does not say connected, verify the ethernet cable is connected to the left-most ethernet port furthest away from the USB port. - For further information, search for Knowledge Base article Reference Images for the NextSeq 1000/2000.

  
• Navigate to IPv4:

  
• Confirm that the following settings are used:

  
• Navigate to the IPv6 and confirm the following settings are used:

  
• Navigate to Security and confirm the following settings are used:

  
• Once enp5s0 (internal NIC) settings are confirmed, verify the enp2s0 (external NIC) settings. Navigate to the enp2s0 similarly to the enp5s0 and confirm the following settings:

  

For any feedback or questions regarding this article (Illumina Knowledge Article #3638), contact Illumina Technical Support [e-mail].