Internal network issues on the NextSeq 1000/2000

Internal network issues on the NextSeq 1000/2000 can result in the following.

• NextSeq 1000/2000 Control Software that is unresponsive, slow, or lagging.

• Initialization failure after power cycling with a timeout error.

Note: If the system has recently undergone networking to a network drive, it is possible the internal network settings may have been changed.

Users may experience the following initialization error: Initialization failed due to failure to connect to client or server. Power cycle the instrument per system guide instructions and retry.

To resolve this type of error, check the internal Network Interface Card (NIC) settings:

• If logged in ilmnuser account, log out of ilmnuser and log into ilmnadmin account to make changes to the system settings.

• From Applications, select System Tools, select Settings, andselect Network.

• Select Settings for enp5s0 (this is the internal NIC).

Example:

• Navigate to IPv4:

  
• Confirm that the following settings are used:

  
• Navigate to the IPv6 and confirm the following settings are used:

  
• Navigate to Security and confirm the following settings are used:

  
• Once enp5s0 (internal NIC) settings are confirmed, check the external NIC settings.

  • Status connected will display for ports with active Ethernet connection (either enp2s0 or enp6s0). - enp2s0 is the preferred Ethernet port for optional second network connection such as NAS or other network storage. (Left port when viewed from back of instrument.) - enp6s0 is the preferred port for internet. (Right port when viewed from the back of instrument.)

  • Navigate to the applicable external NIC, similarly to the enp5s0, and confirm the following settings:

  

For further information, search for Knowledge Base article Reference Images for the NextSeq 1000/2000.

For any feedback or questions regarding this article (Illumina Knowledge Article #3638), contact Illumina Technical Support [e-mail].