Library denaturation for the NextSeq 1000/2000
The NextSeq 1000/2000 system includes automated onboard denature and dilution of libraries, and an off-instrument, manual denaturation option.
On-board denaturation Denaturation and dilution occur within the reagent cartridge. Load double-stranded DNA when using the default on-board denature and dilute protocol.
Off-instrument denaturation If denaturing off-instrument, use Sodium hydroxide (NaOH) and time to complete the denaturation. For more information, search for Knowledge Base article Manual Denaturation of Libraries on the NextSeq 1000/2000.
See the NextSeq 1000 and 2000 Sequencing Systems Guide and NextSeq 1000 and 2000 Denature and Dilute Libraries Guide for more information.
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