# Library denaturation for the NextSeq 1000/2000 The NextSeq 1000/2000 system includes automated onboard denature and dilution of libraries, and an off-instrument, manual denaturation option. **On-board denaturation**\ Denaturation and dilution occur within the reagent cartridge. Load double-stranded DNA when using the default on-board denature and dilute protocol. **Off-instrument denaturation**\ If denaturing off-instrument, use Sodium hydroxide (NaOH) and time to complete the denaturation. For more information, search for Knowledge Base article **Manual Denaturation of Libraries on the NextSeq 1000/2000**. See the [NextSeq 1000 and 2000 Sequencing Systems Guide](https://support-docs.illumina.com/IN/NextSeq10002000/Content/IN/FrontPages/NextSeq10002000.htm) and [NextSeq 1000 and 2000 Denature and Dilute Libraries Guide](https://support-docs.illumina.com/IN/dnd-wizard/Content/dnd/landing.htm?ins=nextseq1k2k) for more information. \ \ \
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