Library denaturation for the NextSeq 1000/2000

The NextSeq 1000/2000 system includes automated onboard denature and dilution of libraries, and an off-instrument, manual denaturation option.

On-board denaturation Denaturation and dilution occur within the reagent cartridge. Load double-stranded DNA when using the default on-board denature and dilute protocol.

Off-instrument denaturation If denaturing off-instrument, use Sodium hydroxide (NaOH) and time to complete the denaturation. For more information, search for Knowledge Base article Manual Denaturation of Libraries on the NextSeq 1000/2000.

See the NextSeq 1000 and 2000 Sequencing Systems Guide and NextSeq 1000 and 2000 Denature and Dilute Libraries Guide for more information.

For any feedback or questions regarding this article (Illumina Knowledge Article #2432), contact Illumina Technical Support [e-mail].