# Library denaturation for the NextSeq 1000/2000

The NextSeq 1000/2000 system includes automated onboard denature and dilution of libraries, and an off-instrument, manual denaturation option.

**On-board denaturation**\
Denaturation and dilution occur within the reagent cartridge. Load double-stranded DNA when using the default on-board denature and dilute protocol.

**Off-instrument denaturation**\
If denaturing off-instrument, use Sodium hydroxide (NaOH) and time to complete the denaturation. For more information, search for Knowledge Base article **Manual Denaturation of Libraries on the NextSeq 1000/2000**.

See the [NextSeq 1000 and 2000 Sequencing Systems Guide](https://support-docs.illumina.com/IN/NextSeq10002000/Content/IN/FrontPages/NextSeq10002000.htm) and [NextSeq 1000 and 2000 Denature and Dilute Libraries Guide](https://support-docs.illumina.com/IN/dnd-wizard/Content/dnd/landing.htm?ins=nextseq1k2k) for more information.

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| *For any feedback or questions regarding this article (Illumina Knowledge Article #2432), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000002432%20-%20Instrumentation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


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